Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.)

Manganese toxicity is one of the growth limiting factors under acidic conditions. Some plants accumulate Mn more in the top than in the root, while others accumulate more in the root than in the top. Vegetable soybean is an indicator plant that can be used to determine the mechanism of toxicity....

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Main Author:	Djafruddin, Rosmaidar
Format:	Thesis
Language:	English English
Published:	2001
Subjects:	Soybean - Case studies
Online Access:	http://psasir.upm.edu.my/id/eprint/10569/1/FP_2001_25.pdf
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id	my-upm-ir.10569
record_format	uketd_dc
spelling	my-upm-ir.105692024-04-17T04:34:52Z Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.) 2001-09 Djafruddin, Rosmaidar Manganese toxicity is one of the growth limiting factors under acidic conditions. Some plants accumulate Mn more in the top than in the root, while others accumulate more in the root than in the top. Vegetable soybean is an indicator plant that can be used to determine the mechanism of toxicity. Part of the plant tissue that accumulates the highest Mn needs to be extracted so as to determine the activity of superoxide dismutase (SOD). In view of this, a series of experiments was conducted to determine the accumulation of Mn in the tissues (leaf, stem, root and pod) of vegetable soybean, toxic level of Mn in solution culture, time-dependent of Mn accumulation in the tissue and the effect of Mn concentration on superoxide dismutase activity under different time of plant growth. Five experiments were conducted in glass house and laboratories at UPM. Experiment 1 was a sand culture experiment to determine the distribution of Mn accumulation in the plant tissues. Experiment 2 and 3 were solution culture experiments to observe toxic level of Mn. Experiment 4 was used to determine time-dependent of Mn accumulation, while experiment 5 was used to study the effect of Mn and time on superoxide dismutase activity. The results showed that the highest accumulation of Mn in vegetable soybean was in the leaves. The Mn concentration corresponding to 20% reduction in dry weight was 20 uM. Plant accumulated Mn more during vegetative stage compared to generative stage. Increasing the time of growth decreased the accumulation of Mn in the 5 and 25 uM of Mn treatments Degeneration of plant cell in toxic condition (25 uM) affected the growth of plant and inhibited nutrient movement from the root to the shoot. Enzyme extraction showed that the activity of superoxide dismutase increased up to 25.36 umol m L-1 of crude enzyme minute-1 at level 15 uM after 20 days of plant age in solution culture. Soybean - Case studies 2001-09 Thesis http://psasir.upm.edu.my/id/eprint/10569/ http://psasir.upm.edu.my/id/eprint/10569/1/FP_2001_25.pdf text en public doctoral Universiti Putra Malaysia Soybean - Case studies Faculty of Agriculture Abdul Rahim, Anuar English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Abdul Rahim, Anuar
topic	Soybean - Case studies
spellingShingle	Soybean - Case studies Djafruddin, Rosmaidar Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.)
description	Manganese toxicity is one of the growth limiting factors under acidic conditions. Some plants accumulate Mn more in the top than in the root, while others accumulate more in the root than in the top. Vegetable soybean is an indicator plant that can be used to determine the mechanism of toxicity. Part of the plant tissue that accumulates the highest Mn needs to be extracted so as to determine the activity of superoxide dismutase (SOD). In view of this, a series of experiments was conducted to determine the accumulation of Mn in the tissues (leaf, stem, root and pod) of vegetable soybean, toxic level of Mn in solution culture, time-dependent of Mn accumulation in the tissue and the effect of Mn concentration on superoxide dismutase activity under different time of plant growth. Five experiments were conducted in glass house and laboratories at UPM. Experiment 1 was a sand culture experiment to determine the distribution of Mn accumulation in the plant tissues. Experiment 2 and 3 were solution culture experiments to observe toxic level of Mn. Experiment 4 was used to determine time-dependent of Mn accumulation, while experiment 5 was used to study the effect of Mn and time on superoxide dismutase activity. The results showed that the highest accumulation of Mn in vegetable soybean was in the leaves. The Mn concentration corresponding to 20% reduction in dry weight was 20 uM. Plant accumulated Mn more during vegetative stage compared to generative stage. Increasing the time of growth decreased the accumulation of Mn in the 5 and 25 uM of Mn treatments Degeneration of plant cell in toxic condition (25 uM) affected the growth of plant and inhibited nutrient movement from the root to the shoot. Enzyme extraction showed that the activity of superoxide dismutase increased up to 25.36 umol m L-1 of crude enzyme minute-1 at level 15 uM after 20 days of plant age in solution culture.
format	Thesis
qualification_level	Doctorate
author	Djafruddin, Rosmaidar
author_facet	Djafruddin, Rosmaidar
author_sort	Djafruddin, Rosmaidar
title	Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.)
title_short	Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.)
title_full	Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.)
title_fullStr	Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.)
title_full_unstemmed	Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.)
title_sort	manganese accumulation and toxicity, and superoxide dismutase activity in vegetable soybean (glycine max (l.) merr.)
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Agriculture
publishDate	2001
url	http://psasir.upm.edu.my/id/eprint/10569/1/FP_2001_25.pdf
_version_	1804888569851936768

Manganese Accumulation and Toxicity, and Superoxide Dismutase Activity in Vegetable Soybean (Glycine Max (L.) Merr.)

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