Phenotypic Variability of Brachiaria Decumbens After Exposure to Gamma Irradiation

Brachiaria decumbens (Signal grass) has been used for many years as grazing pastures for ruminants. Its toxicity to small ruminants is the main limitation to utilization of this grass. Many researchers have reported several methods to control or alleviate this problem. Since there is a potential...

Full description

Saved in:

Bibliographic Details
Main Author:	Hussin, Ghazalli
Format:	Thesis
Language:	English English
Published:	2003
Subjects:	Bracharia decumbens - Gamma rays - Case studies
Online Access:	http://psasir.upm.edu.my/id/eprint/10632/1/FP_2003_1.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.10632
record_format	uketd_dc
spelling	my-upm-ir.106322024-04-30T04:44:11Z Phenotypic Variability of Brachiaria Decumbens After Exposure to Gamma Irradiation 2003-01 Hussin, Ghazalli Brachiaria decumbens (Signal grass) has been used for many years as grazing pastures for ruminants. Its toxicity to small ruminants is the main limitation to utilization of this grass. Many researchers have reported several methods to control or alleviate this problem. Since there is a potential to use mutagenesis techniques to obtain variability of the plants, the objective of this study is to observe the phenotypic variability of Signal grass after mutagenic induction with gamma radiation. Further studies can be conducted to make a selection of variants which are free from toxicity. Brachiaria decumbens seeds were irradiated at doses of 1 00, 200, 300, 400, 500, 600, 700, 800 and 900 Gy to determine the appropriate doses for mutagenic treatments of the grass. The results showed that, B. decumbens seeds were less sensitive to gamma ray than most other species and the LD50 was found to be between 800 to 900 Oy. In order to observe the effects of gamma radiation on phenotypic variability of the grass, the grass seeds were exposed to 900 Oy gamma radiation. Results showed that gamma radiation at 900 Oy increased variability in morphological characteristics and nutrient contents and an obvious phenotypic mutant was detected. There were significant increases in variabilities of morphological characters (tiller numbers, leaf length, leaf width, leaf weights, stem weights., leaf-to-stem ratio and internode lengths) and nutrient contents (Crude Protein, Ether Extract, Crude Fibre, Acid Detergent Fibre, Neutral Detergent Fibre and Ash) among the treated plants. There was also a positive skew of the frequency distribution curve for the treated plants to the right, indicating that mutagenic radiation can cause increased variability, which allows greater selection potential for desired characteristics. DNA Polymorphism in the mutant cannot be detected through RAPD. The mutant showed significantly higher leaf width, leaf-to-stem ratio and crude protein compared to control plants. Bracharia decumbens - Gamma rays - Case studies 2003-01 Thesis http://psasir.upm.edu.my/id/eprint/10632/ http://psasir.upm.edu.my/id/eprint/10632/1/FP_2003_1.pdf text en public masters Universiti Putra Malaysia Bracharia decumbens - Gamma rays - Case studies Faculty of Agriculture English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Bracharia decumbens - Gamma rays - Case studies
spellingShingle	Bracharia decumbens - Gamma rays - Case studies Hussin, Ghazalli Phenotypic Variability of Brachiaria Decumbens After Exposure to Gamma Irradiation
description	Brachiaria decumbens (Signal grass) has been used for many years as grazing pastures for ruminants. Its toxicity to small ruminants is the main limitation to utilization of this grass. Many researchers have reported several methods to control or alleviate this problem. Since there is a potential to use mutagenesis techniques to obtain variability of the plants, the objective of this study is to observe the phenotypic variability of Signal grass after mutagenic induction with gamma radiation. Further studies can be conducted to make a selection of variants which are free from toxicity. Brachiaria decumbens seeds were irradiated at doses of 1 00, 200, 300, 400, 500, 600, 700, 800 and 900 Gy to determine the appropriate doses for mutagenic treatments of the grass. The results showed that, B. decumbens seeds were less sensitive to gamma ray than most other species and the LD50 was found to be between 800 to 900 Oy. In order to observe the effects of gamma radiation on phenotypic variability of the grass, the grass seeds were exposed to 900 Oy gamma radiation. Results showed that gamma radiation at 900 Oy increased variability in morphological characteristics and nutrient contents and an obvious phenotypic mutant was detected. There were significant increases in variabilities of morphological characters (tiller numbers, leaf length, leaf width, leaf weights, stem weights., leaf-to-stem ratio and internode lengths) and nutrient contents (Crude Protein, Ether Extract, Crude Fibre, Acid Detergent Fibre, Neutral Detergent Fibre and Ash) among the treated plants. There was also a positive skew of the frequency distribution curve for the treated plants to the right, indicating that mutagenic radiation can cause increased variability, which allows greater selection potential for desired characteristics. DNA Polymorphism in the mutant cannot be detected through RAPD. The mutant showed significantly higher leaf width, leaf-to-stem ratio and crude protein compared to control plants.
format	Thesis
qualification_level	Master's degree
author	Hussin, Ghazalli
author_facet	Hussin, Ghazalli
author_sort	Hussin, Ghazalli
title	Phenotypic Variability of Brachiaria Decumbens After Exposure to Gamma Irradiation
title_short	Phenotypic Variability of Brachiaria Decumbens After Exposure to Gamma Irradiation
title_full	Phenotypic Variability of Brachiaria Decumbens After Exposure to Gamma Irradiation
title_fullStr	Phenotypic Variability of Brachiaria Decumbens After Exposure to Gamma Irradiation
title_full_unstemmed	Phenotypic Variability of Brachiaria Decumbens After Exposure to Gamma Irradiation
title_sort	phenotypic variability of brachiaria decumbens after exposure to gamma irradiation
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Agriculture
publishDate	2003
url	http://psasir.upm.edu.my/id/eprint/10632/1/FP_2003_1.pdf
_version_	1804888581429264384

Phenotypic Variability of Brachiaria Decumbens After Exposure to Gamma Irradiation

Similar Items