Identification and Pathogenicity of Cladosporium Musae Mason from Banana Leaf Speckle

A study was undertaken to isolate and identify Cladosporium sp. from banana leaves associated with a speckle disease which is considered a serious problem especially in 'Berangan' and 'Mas' cultivars planted in Teluk Intan, Perak 0/Vest Malaysia). Direct plating of infected &...

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Main Author:	Sahlan
Format:	Thesis
Language:	English English
Published:	2003
Subjects:	Cladosporium - Identification - Case studies
Online Access:	http://psasir.upm.edu.my/id/eprint/10633/1/FP_2003_2_A.pdf
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id	my-upm-ir.10633
record_format	uketd_dc
spelling	my-upm-ir.106332011-08-10T05:46:18Z Identification and Pathogenicity of Cladosporium Musae Mason from Banana Leaf Speckle 2003-04 Sahlan, A study was undertaken to isolate and identify Cladosporium sp. from banana leaves associated with a speckle disease which is considered a serious problem especially in 'Berangan' and 'Mas' cultivars planted in Teluk Intan, Perak 0/Vest Malaysia). Direct plating of infected 'Berangan' banana leaves surface sterilized in 5% sodium hypochlorite (NaOCI) solution on Potato Dextrose Agar (PDA) failed to isolate Cladosporium sp. However, pure cultures were successfully obtained by a single spore isolation method. Conidia from diseased leaves were first scrapped using a fine paint brush into sterile water. The conidial suspension was then poured over 2% water agar 0/VA) incorporated with antibiotics (streptomycin and tetracycline sulphate) and incubated for 24 hours at ambient temperature. A single germinated conidia was picked with an inoculation needle and transferred onto a PDA plate similarly a mended with antibiotics. The fungus produced erect conidiophores and conidia which were mainly fusiform and aseptate or 1-septate on banana leaves. Conidiophores on Malt Extract Agar (MEA) were straight and flexuous, and produced conidia and ramoconidia which were very variable in shape and size. A comparison between the morphological characteristics and dimensions of the Teluk Intan fungus on banana leaves and culture medium with those previously described in literature suggest its close similarity and synonymous identification with Cladosporium musae Mason. The best culture medium for growth of C. musae Mason was on Banana Leaf Extract Agar (BLEA), while MEA and PDA were the best culture medium for supporting maximum conidia production. In culture, it can grow between a temperature range of 18°C to 30°C, but the optimum temperatures for mycelial growth and sporulation were 22°C and 26°c respectively. The optimum temperature for conidial germination was 26°C combined with relative humidity (RH) of between 99% to 100%. Cladosporium - Identification - Case studies 2003-04 Thesis http://psasir.upm.edu.my/id/eprint/10633/ http://psasir.upm.edu.my/id/eprint/10633/1/FP_2003_2_A.pdf application/pdf en public masters Universiti Putra Malaysia Cladosporium - Identification - Case studies Faculty of Agriculture English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Cladosporium - Identification - Case studies
spellingShingle	Cladosporium - Identification - Case studies Sahlan, Identification and Pathogenicity of Cladosporium Musae Mason from Banana Leaf Speckle
description	A study was undertaken to isolate and identify Cladosporium sp. from banana leaves associated with a speckle disease which is considered a serious problem especially in 'Berangan' and 'Mas' cultivars planted in Teluk Intan, Perak 0/Vest Malaysia). Direct plating of infected 'Berangan' banana leaves surface sterilized in 5% sodium hypochlorite (NaOCI) solution on Potato Dextrose Agar (PDA) failed to isolate Cladosporium sp. However, pure cultures were successfully obtained by a single spore isolation method. Conidia from diseased leaves were first scrapped using a fine paint brush into sterile water. The conidial suspension was then poured over 2% water agar 0/VA) incorporated with antibiotics (streptomycin and tetracycline sulphate) and incubated for 24 hours at ambient temperature. A single germinated conidia was picked with an inoculation needle and transferred onto a PDA plate similarly a mended with antibiotics. The fungus produced erect conidiophores and conidia which were mainly fusiform and aseptate or 1-septate on banana leaves. Conidiophores on Malt Extract Agar (MEA) were straight and flexuous, and produced conidia and ramoconidia which were very variable in shape and size. A comparison between the morphological characteristics and dimensions of the Teluk Intan fungus on banana leaves and culture medium with those previously described in literature suggest its close similarity and synonymous identification with Cladosporium musae Mason. The best culture medium for growth of C. musae Mason was on Banana Leaf Extract Agar (BLEA), while MEA and PDA were the best culture medium for supporting maximum conidia production. In culture, it can grow between a temperature range of 18°C to 30°C, but the optimum temperatures for mycelial growth and sporulation were 22°C and 26°c respectively. The optimum temperature for conidial germination was 26°C combined with relative humidity (RH) of between 99% to 100%.
format	Thesis
qualification_level	Master's degree
author	Sahlan,
author_facet	Sahlan,
author_sort	Sahlan,
title	Identification and Pathogenicity of Cladosporium Musae Mason from Banana Leaf Speckle
title_short	Identification and Pathogenicity of Cladosporium Musae Mason from Banana Leaf Speckle
title_full	Identification and Pathogenicity of Cladosporium Musae Mason from Banana Leaf Speckle
title_fullStr	Identification and Pathogenicity of Cladosporium Musae Mason from Banana Leaf Speckle
title_full_unstemmed	Identification and Pathogenicity of Cladosporium Musae Mason from Banana Leaf Speckle
title_sort	identification and pathogenicity of cladosporium musae mason from banana leaf speckle
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Agriculture
publishDate	2003
url	http://psasir.upm.edu.my/id/eprint/10633/1/FP_2003_2_A.pdf
_version_	1747811164725706752

Identification and Pathogenicity of Cladosporium Musae Mason from Banana Leaf Speckle

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