Quantification and Characterization of Trichoderma Spp. from Different Habitats
The abundance of Trichoderma was not significantly different between the oil palm cultivated areas and jungle areas. Soil pH and soil moisture content did not have an effect on the abundance of Trichoderma in the areas sampled. Ganoderma infected area with percentage disease incidence (PDI) of>...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2003
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/10635/1/FP_2003_4.pdf |
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| Summary: | The abundance of Trichoderma was not significantly different between
the oil palm cultivated areas and jungle areas. Soil pH and soil moisture content
did not have an effect on the abundance of Trichoderma in the areas sampled.
Ganoderma infected area with percentage disease incidence (PDI) of> 30%
recorded higher frerquency (9.5 x 103 cfu/g air dried soil) of isolation of
Trichoderma. In the reserved forest habitat, inland soil seemed to harbor higher
population (10.9 x 103 cfu/g dried soil) of Trichoderma. Generally for all
habitats and areas sampled, the two upper soil horizons (AI and Be) supported
higher population of Trichoderma and the distribution decreased with depth of
soil. However, in the EFB mulched area there was a significant increase in
Trichoderma with increase in depth of profile. Based on phenotype
appearances, four species aggregates of Trichoderma were identified from oil
palm and forest rhizospheres, namely T. harzianum, T virens, T koningii, and T. longibrachiatum. T. harzianum and r virens were the most frequently
isolated species aggregates while T longibrachiatum was the least. The
variation between species aggregates of Trichoderma was distinguished by
using RAPD. However, overlapping was found between T. virens and T.
koningii and T. longibrachiatum within a main cluster. Isolates of the same
species were group together within the same sub cluster indicating a close
genetic linkage among the same species. Several putative DNA markers were
identified that could be used for interspecies differentiation if consecutive PCR
tests were carried out with primer OPC-II and OPC-I5. Confrontation assay
based on percentage inhibition of mycelial growth and c010ny overgrowth
showed that there were variations in the degree of antagonistic ability between
and within species aggregates of Trichoderma. The mode of action was
attributed to competition, mycoparasitism and / or antibiosis. Isolates TH80 of
T. harzianum, TK126 of T. koningii and TV26 of T. virens were found to be the
most effective antagonists. |
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