Quantification and Characterization of Trichoderma Spp. from Different Habitats

The abundance of Trichoderma was not significantly different between the oil palm cultivated areas and jungle areas. Soil pH and soil moisture content did not have an effect on the abundance of Trichoderma in the areas sampled. Ganoderma infected area with percentage disease incidence (PDI) of>...

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Main Author:	Choo, Chee Wei
Format:	Thesis
Language:	English English
Published:	2003
Subjects:	Trichoderma - Case studies
Online Access:	http://psasir.upm.edu.my/id/eprint/10635/1/FP_2003_4.pdf
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id	my-upm-ir.10635
record_format	uketd_dc
spelling	my-upm-ir.106352024-04-30T08:36:47Z Quantification and Characterization of Trichoderma Spp. from Different Habitats 2003-11 Choo, Chee Wei The abundance of Trichoderma was not significantly different between the oil palm cultivated areas and jungle areas. Soil pH and soil moisture content did not have an effect on the abundance of Trichoderma in the areas sampled. Ganoderma infected area with percentage disease incidence (PDI) of> 30% recorded higher frerquency (9.5 x 103 cfu/g air dried soil) of isolation of Trichoderma. In the reserved forest habitat, inland soil seemed to harbor higher population (10.9 x 103 cfu/g dried soil) of Trichoderma. Generally for all habitats and areas sampled, the two upper soil horizons (AI and Be) supported higher population of Trichoderma and the distribution decreased with depth of soil. However, in the EFB mulched area there was a significant increase in Trichoderma with increase in depth of profile. Based on phenotype appearances, four species aggregates of Trichoderma were identified from oil palm and forest rhizospheres, namely T. harzianum, T virens, T koningii, and T. longibrachiatum. T. harzianum and r virens were the most frequently isolated species aggregates while T longibrachiatum was the least. The variation between species aggregates of Trichoderma was distinguished by using RAPD. However, overlapping was found between T. virens and T. koningii and T. longibrachiatum within a main cluster. Isolates of the same species were group together within the same sub cluster indicating a close genetic linkage among the same species. Several putative DNA markers were identified that could be used for interspecies differentiation if consecutive PCR tests were carried out with primer OPC-II and OPC-I5. Confrontation assay based on percentage inhibition of mycelial growth and c010ny overgrowth showed that there were variations in the degree of antagonistic ability between and within species aggregates of Trichoderma. The mode of action was attributed to competition, mycoparasitism and / or antibiosis. Isolates TH80 of T. harzianum, TK126 of T. koningii and TV26 of T. virens were found to be the most effective antagonists. Trichoderma - Case studies 2003-11 Thesis http://psasir.upm.edu.my/id/eprint/10635/ http://psasir.upm.edu.my/id/eprint/10635/1/FP_2003_4.pdf text en public masters Universiti Putra Malaysia Trichoderma - Case studies Faculty of Agriculture Meon, Sariah English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Meon, Sariah
topic	Trichoderma - Case studies
spellingShingle	Trichoderma - Case studies Choo, Chee Wei Quantification and Characterization of Trichoderma Spp. from Different Habitats
description	The abundance of Trichoderma was not significantly different between the oil palm cultivated areas and jungle areas. Soil pH and soil moisture content did not have an effect on the abundance of Trichoderma in the areas sampled. Ganoderma infected area with percentage disease incidence (PDI) of> 30% recorded higher frerquency (9.5 x 103 cfu/g air dried soil) of isolation of Trichoderma. In the reserved forest habitat, inland soil seemed to harbor higher population (10.9 x 103 cfu/g dried soil) of Trichoderma. Generally for all habitats and areas sampled, the two upper soil horizons (AI and Be) supported higher population of Trichoderma and the distribution decreased with depth of soil. However, in the EFB mulched area there was a significant increase in Trichoderma with increase in depth of profile. Based on phenotype appearances, four species aggregates of Trichoderma were identified from oil palm and forest rhizospheres, namely T. harzianum, T virens, T koningii, and T. longibrachiatum. T. harzianum and r virens were the most frequently isolated species aggregates while T longibrachiatum was the least. The variation between species aggregates of Trichoderma was distinguished by using RAPD. However, overlapping was found between T. virens and T. koningii and T. longibrachiatum within a main cluster. Isolates of the same species were group together within the same sub cluster indicating a close genetic linkage among the same species. Several putative DNA markers were identified that could be used for interspecies differentiation if consecutive PCR tests were carried out with primer OPC-II and OPC-I5. Confrontation assay based on percentage inhibition of mycelial growth and c010ny overgrowth showed that there were variations in the degree of antagonistic ability between and within species aggregates of Trichoderma. The mode of action was attributed to competition, mycoparasitism and / or antibiosis. Isolates TH80 of T. harzianum, TK126 of T. koningii and TV26 of T. virens were found to be the most effective antagonists.
format	Thesis
qualification_level	Master's degree
author	Choo, Chee Wei
author_facet	Choo, Chee Wei
author_sort	Choo, Chee Wei
title	Quantification and Characterization of Trichoderma Spp. from Different Habitats
title_short	Quantification and Characterization of Trichoderma Spp. from Different Habitats
title_full	Quantification and Characterization of Trichoderma Spp. from Different Habitats
title_fullStr	Quantification and Characterization of Trichoderma Spp. from Different Habitats
title_full_unstemmed	Quantification and Characterization of Trichoderma Spp. from Different Habitats
title_sort	quantification and characterization of trichoderma spp. from different habitats
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Agriculture
publishDate	2003
url	http://psasir.upm.edu.my/id/eprint/10635/1/FP_2003_4.pdf
_version_	1804888582185287680

Quantification and Characterization of Trichoderma Spp. from Different Habitats

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