Effects of Selected Plant Growth Regulators on Regeneration of Mangosteen (Garcinia Mangostana L.) Using Tissue Culture Technique

Effects of Selected Plant Growth Regulators on Regeneration of Mangosteen (Garcinia Mangostana L.) Using Tissue Culture Technique

The in-vitro plantlet regeneration system via organogenesis and callus induction was developed from various mangosteen explants (seed, shoot tip, stem and leaf) using various plant growth regulators (PGR). For shoot induction, the mangosteen half seed, leaf and stem explants sufficiently achieved...

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Main Author: Sirchi, Mohammad Hossein Torabi
Format: Thesis
Language:English
English
Published: 2009
Subjects:
Mangosteen - Growth - Case studies
Online Access:http://psasir.upm.edu.my/id/eprint/10698/1/FP_2009_6.pdf
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spelling my-upm-ir.106982024-05-13T07:27:40Z Effects of Selected Plant Growth Regulators on Regeneration of Mangosteen (Garcinia Mangostana L.) Using Tissue Culture Technique 2009-05 Sirchi, Mohammad Hossein Torabi The in-vitro plantlet regeneration system via organogenesis and callus induction was developed from various mangosteen explants (seed, shoot tip, stem and leaf) using various plant growth regulators (PGR). For shoot induction, the mangosteen half seed, leaf and stem explants sufficiently achieved shoot regeneration rates above 60% of total explants cultured (70%, 60% and 60% respectively) on various auxin and cytokinin supplementation into the basal MS medium [4.0 mgL-1 (w/v) BAP with 0.2 mgL-l (w/v) NAA for seed; 1.0 mgL-l (w/v) BAP in combination with 0.05 mgL-1 (w/v) KIN for stem; 2.0 mgL-l (w/v) of BAP for leaf]. Shoot induction from the shoot tip explant was not successful. However, among the three explants, the leaf explant gave the highest mean number of s hoots at 46.5 ( seed and stem at 9.3 and 8 . 9 respectively). Mean shoot height h ow ever, was 0.48 em for leaf, while it was h igher for shoots generated from stem and seed explants (0.7 em and 0.8 em respectively). The shoots rooted successfully o n ful l strength M S medium w ithout IBA w i th high percentage of root formation (87%) and number of roots ( 3 . 8 ) (seven months after culture). The p l an tlets were then successfully potted and acclimatized o n medium containing soil + sand+ organic matter+ vermiculite m i x ture (2:2: 1: 1) which gave the h ighest n umber of l eaves (6.5), plant height (3.5cm) and percentage of plant survival ( 8 0%). As for callus induction, i nduction from seed and leaves w as 90% on medium with 0.5 mgL-' (w/v) NAA, whi le i nduction from shoot tip and stem was 90% and 83% respectively on medium with 0.2 mgL-' ( w/v) KIN. Our study has successfully developed a plant regeneration s ystem from e asil y avai lable stem and green juvenile leaf explants, as compared to the seasonall y available and limited seed and you n g leaf expl ants that are generall y reported by other researches. Furthermore, callus induction was successfu l from various mangosteen explants for future uti lization in the development of a plant regeneration protocol from callus, as callus also has the potential of producing improved varieties via various genetic modification techniques today. Mangosteen - Growth - Case studies 2009-05 Thesis http://psasir.upm.edu.my/id/eprint/10698/ http://psasir.upm.edu.my/id/eprint/10698/1/FP_2009_6.pdf text en public masters Universiti Putra Malaysia Mangosteen - Growth - Case studies Faculty of Agriculture Abdul Kadir, Mihdzar English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
advisor Abdul Kadir, Mihdzar
topic Mangosteen - Growth - Case studies
spellingShingle Mangosteen - Growth - Case studies


Sirchi, Mohammad Hossein Torabi
Effects of Selected Plant Growth Regulators on Regeneration of Mangosteen (Garcinia Mangostana L.) Using Tissue Culture Technique
description The in-vitro plantlet regeneration system via organogenesis and callus induction was developed from various mangosteen explants (seed, shoot tip, stem and leaf) using various plant growth regulators (PGR). For shoot induction, the mangosteen half seed, leaf and stem explants sufficiently achieved shoot regeneration rates above 60% of total explants cultured (70%, 60% and 60% respectively) on various auxin and cytokinin supplementation into the basal MS medium [4.0 mgL-1 (w/v) BAP with 0.2 mgL-l (w/v) NAA for seed; 1.0 mgL-l (w/v) BAP in combination with 0.05 mgL-1 (w/v) KIN for stem; 2.0 mgL-l (w/v) of BAP for leaf]. Shoot induction from the shoot tip explant was not successful. However, among the three explants, the leaf explant gave the highest mean number of s hoots at 46.5 ( seed and stem at 9.3 and 8 . 9 respectively). Mean shoot height h ow ever, was 0.48 em for leaf, while it was h igher for shoots generated from stem and seed explants (0.7 em and 0.8 em respectively). The shoots rooted successfully o n ful l strength M S medium w ithout IBA w i th high percentage of root formation (87%) and number of roots ( 3 . 8 ) (seven months after culture). The p l an tlets were then successfully potted and acclimatized o n medium containing soil + sand+ organic matter+ vermiculite m i x ture (2:2: 1: 1) which gave the h ighest n umber of l eaves (6.5), plant height (3.5cm) and percentage of plant survival ( 8 0%). As for callus induction, i nduction from seed and leaves w as 90% on medium with 0.5 mgL-' (w/v) NAA, whi le i nduction from shoot tip and stem was 90% and 83% respectively on medium with 0.2 mgL-' ( w/v) KIN. Our study has successfully developed a plant regeneration s ystem from e asil y avai lable stem and green juvenile leaf explants, as compared to the seasonall y available and limited seed and you n g leaf expl ants that are generall y reported by other researches. Furthermore, callus induction was successfu l from various mangosteen explants for future uti lization in the development of a plant regeneration protocol from callus, as callus also has the potential of producing improved varieties via various genetic modification techniques today.
format Thesis
qualification_level Master's degree
author Sirchi, Mohammad Hossein Torabi
author_facet Sirchi, Mohammad Hossein Torabi
author_sort Sirchi, Mohammad Hossein Torabi
title Effects of Selected Plant Growth Regulators on Regeneration of Mangosteen (Garcinia Mangostana L.) Using Tissue Culture Technique
title_short Effects of Selected Plant Growth Regulators on Regeneration of Mangosteen (Garcinia Mangostana L.) Using Tissue Culture Technique
title_full Effects of Selected Plant Growth Regulators on Regeneration of Mangosteen (Garcinia Mangostana L.) Using Tissue Culture Technique
title_fullStr Effects of Selected Plant Growth Regulators on Regeneration of Mangosteen (Garcinia Mangostana L.) Using Tissue Culture Technique
title_full_unstemmed Effects of Selected Plant Growth Regulators on Regeneration of Mangosteen (Garcinia Mangostana L.) Using Tissue Culture Technique
title_sort effects of selected plant growth regulators on regeneration of mangosteen (garcinia mangostana l.) using tissue culture technique
granting_institution Universiti Putra Malaysia
granting_department Faculty of Agriculture
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/10698/1/FP_2009_6.pdf
_version_ 1804888595619643392

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