Pathogenicity Of Pasteurella Multocida Serotypes A:3, D:1 And D:3 In Rabbits

Eighty-two isolates of P. multocida were recovered from healthy and diseased rabbits in a rabbitry at Universiti Putra Malaysia. S ixty three isolates were obtained from adult rabbits and 1 9 isolates were from juvenile rabbits. Bacterial identification by biochemical tests, capsular and somatic...

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Main Author:	Al-Haddawi, Muthafar Hussain
Format:	Thesis
Language:	English English
Published:	1999
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Online Access:	http://psasir.upm.edu.my/id/eprint/11062/1/FPV_1999_9_A.pdf
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id	my-upm-ir.11062
record_format	uketd_dc
spelling	my-upm-ir.110622013-07-18T05:29:00Z Pathogenicity Of Pasteurella Multocida Serotypes A:3, D:1 And D:3 In Rabbits 1999-08 Al-Haddawi, Muthafar Hussain Eighty-two isolates of P. multocida were recovered from healthy and diseased rabbits in a rabbitry at Universiti Putra Malaysia. S ixty three isolates were obtained from adult rabbits and 1 9 isolates were from juvenile rabbits. Bacterial identification by biochemical tests, capsular and somatic serotyping and sensitivity to the antimicrobials were conducted. Capsular typing revealed that the major capsular type was type A (89%), while type D was less frequent ( 1 1 %). Somatic serotyping of 46 isolates showed that serotypes 1 and 3 were more frequent among the capsular types. Most isolates were sensitive to several common antimicrobials but some were resistant to neomycin, penicillin, cloxacillin and tetracycline. Six of 40 isolates of P. multocida from healthy ( 1 8 isolates) and diseased (22 isolates) rabbits were found to harbour plasmids. There was no correlation between presence of plasmids and serotype, resistance to antimicrobial agents and from the site which the bacteria were cultured. Random amplification polymorphic DNA (RAPD) was applied to subtype 40 P. multocida isolates using two single primers to test their abilities to generate individual fingerprints. A wide heterogeneity within the isolates was observed. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of the 40 isolates revealed over 40 protein bands with high molecular weight bands indicating striking homogeneity among the isolates. Twenty-two different patterns were observed. 1999-08 Thesis http://psasir.upm.edu.my/id/eprint/11062/ http://psasir.upm.edu.my/id/eprint/11062/1/FPV_1999_9_A.pdf application/pdf en public phd doctoral Universiti Putra Malaysia Faculty of Veterinary Medicine English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic
spellingShingle	Al-Haddawi, Muthafar Hussain Pathogenicity Of Pasteurella Multocida Serotypes A:3, D:1 And D:3 In Rabbits
description	Eighty-two isolates of P. multocida were recovered from healthy and diseased rabbits in a rabbitry at Universiti Putra Malaysia. S ixty three isolates were obtained from adult rabbits and 1 9 isolates were from juvenile rabbits. Bacterial identification by biochemical tests, capsular and somatic serotyping and sensitivity to the antimicrobials were conducted. Capsular typing revealed that the major capsular type was type A (89%), while type D was less frequent ( 1 1 %). Somatic serotyping of 46 isolates showed that serotypes 1 and 3 were more frequent among the capsular types. Most isolates were sensitive to several common antimicrobials but some were resistant to neomycin, penicillin, cloxacillin and tetracycline. Six of 40 isolates of P. multocida from healthy ( 1 8 isolates) and diseased (22 isolates) rabbits were found to harbour plasmids. There was no correlation between presence of plasmids and serotype, resistance to antimicrobial agents and from the site which the bacteria were cultured. Random amplification polymorphic DNA (RAPD) was applied to subtype 40 P. multocida isolates using two single primers to test their abilities to generate individual fingerprints. A wide heterogeneity within the isolates was observed. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of the 40 isolates revealed over 40 protein bands with high molecular weight bands indicating striking homogeneity among the isolates. Twenty-two different patterns were observed.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Al-Haddawi, Muthafar Hussain
author_facet	Al-Haddawi, Muthafar Hussain
author_sort	Al-Haddawi, Muthafar Hussain
title	Pathogenicity Of Pasteurella Multocida Serotypes A:3, D:1 And D:3 In Rabbits
title_short	Pathogenicity Of Pasteurella Multocida Serotypes A:3, D:1 And D:3 In Rabbits
title_full	Pathogenicity Of Pasteurella Multocida Serotypes A:3, D:1 And D:3 In Rabbits
title_fullStr	Pathogenicity Of Pasteurella Multocida Serotypes A:3, D:1 And D:3 In Rabbits
title_full_unstemmed	Pathogenicity Of Pasteurella Multocida Serotypes A:3, D:1 And D:3 In Rabbits
title_sort	pathogenicity of pasteurella multocida serotypes a:3, d:1 and d:3 in rabbits
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Veterinary Medicine
publishDate	1999
url	http://psasir.upm.edu.my/id/eprint/11062/1/FPV_1999_9_A.pdf
_version_	1747811198719492096

Pathogenicity Of Pasteurella Multocida Serotypes A:3, D:1 And D:3 In Rabbits

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