Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus
Twenty isolates of Vibrio cholerae and four isolates of Vibrio alginolyticus used in this study were identified using the conventional biochemical tests. All of the isolates were screened for the zonula occludens toxin gene (zot) by the Polymerase Chain Reaction (PCR) technique. The 1083 bp fragm...
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2000
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my-upm-ir.112092024-06-12T04:38:58Z Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus 2000-05 Neela, Vasanthakumari Twenty isolates of Vibrio cholerae and four isolates of Vibrio alginolyticus used in this study were identified using the conventional biochemical tests. All of the isolates were screened for the zonula occludens toxin gene (zot) by the Polymerase Chain Reaction (PCR) technique. The 1083 bp fragment of the zot gene was successfully amplified in all the tested isolates. Asymmetry PCR was used as a preliminary detection method and later confirmed through the gene probe method to test for the presence of the zot gene. Consequently, the zot gene was successfully cloned in pCR 2.1 TOPO vector and sequenced, in which the sequences were 99% homologous to the gene bank sequences. Vibrio cholerae Vibrionaceae Gram negative bacteria 2000-05 Thesis http://psasir.upm.edu.my/id/eprint/11209/ http://psasir.upm.edu.my/id/eprint/11209/1/FPSK_M_2000_6.pdf text en public masters Universiti Putra Malaysia Vibrio cholerae Vibrionaceae Gram negative bacteria Faculty of Medicine and Health Science Nor Shamsudin, Mariana English |
institution |
Universiti Putra Malaysia |
collection |
PSAS Institutional Repository |
language |
English English |
advisor |
Nor Shamsudin, Mariana |
topic |
Vibrio cholerae Vibrionaceae Gram negative bacteria |
spellingShingle |
Vibrio cholerae Vibrionaceae Gram negative bacteria Neela, Vasanthakumari Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus |
description |
Twenty isolates of Vibrio cholerae and four isolates of Vibrio alginolyticus used
in this study were identified using the conventional biochemical tests. All of the isolates
were screened for the zonula occludens toxin gene (zot) by the Polymerase Chain
Reaction (PCR) technique. The 1083 bp fragment of the zot gene was successfully
amplified in all the tested isolates. Asymmetry PCR was used as a preliminary detection
method and later confirmed through the gene probe method to test for the presence of the
zot gene. Consequently, the zot gene was successfully cloned in pCR 2.1 TOPO vector
and sequenced, in which the sequences were 99% homologous to the gene bank
sequences. |
format |
Thesis |
qualification_level |
Master's degree |
author |
Neela, Vasanthakumari |
author_facet |
Neela, Vasanthakumari |
author_sort |
Neela, Vasanthakumari |
title |
Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus |
title_short |
Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus |
title_full |
Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus |
title_fullStr |
Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus |
title_full_unstemmed |
Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus |
title_sort |
molecular and immunological characteristics of vibrio cholerae and vibrio alginolyticus |
granting_institution |
Universiti Putra Malaysia |
granting_department |
Faculty of Medicine and Health Science |
publishDate |
2000 |
url |
http://psasir.upm.edu.my/id/eprint/11209/1/FPSK_M_2000_6.pdf |
_version_ |
1804888624607526912 |