Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus

Twenty isolates of Vibrio cholerae and four isolates of Vibrio alginolyticus used in this study were identified using the conventional biochemical tests. All of the isolates were screened for the zonula occludens toxin gene (zot) by the Polymerase Chain Reaction (PCR) technique. The 1083 bp fragm...

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Main Author:	Neela, Vasanthakumari
Format:	Thesis
Language:	English English
Published:	2000
Subjects:	Vibrio cholerae Vibrionaceae Gram negative bacteria
Online Access:	http://psasir.upm.edu.my/id/eprint/11209/1/FPSK_M_2000_6_A.pdf
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id	my-upm-ir.11209
record_format	uketd_dc
spelling	my-upm-ir.112092011-08-16T02:19:44Z Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus 2000-05 Neela, Vasanthakumari Twenty isolates of Vibrio cholerae and four isolates of Vibrio alginolyticus used in this study were identified using the conventional biochemical tests. All of the isolates were screened for the zonula occludens toxin gene (zot) by the Polymerase Chain Reaction (PCR) technique. The 1083 bp fragment of the zot gene was successfully amplified in all the tested isolates. Asymmetry PCR was used as a preliminary detection method and later confirmed through the gene probe method to test for the presence of the zot gene. Consequently, the zot gene was successfully cloned in pCR 2.1 TOPO vector and sequenced, in which the sequences were 99% homologous to the gene bank sequences. Vibrio cholerae Vibrionaceae Gram negative bacteria 2000-05 Thesis http://psasir.upm.edu.my/id/eprint/11209/ http://psasir.upm.edu.my/id/eprint/11209/1/FPSK_M_2000_6_A.pdf application/pdf en public masters Universiti Putra Malaysia Vibrio cholerae Vibrionaceae Gram negative bacteria Faculty of Medicine and Health Science English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Vibrio cholerae Vibrionaceae Gram negative bacteria
spellingShingle	Vibrio cholerae Vibrionaceae Gram negative bacteria Neela, Vasanthakumari Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus
description	Twenty isolates of Vibrio cholerae and four isolates of Vibrio alginolyticus used in this study were identified using the conventional biochemical tests. All of the isolates were screened for the zonula occludens toxin gene (zot) by the Polymerase Chain Reaction (PCR) technique. The 1083 bp fragment of the zot gene was successfully amplified in all the tested isolates. Asymmetry PCR was used as a preliminary detection method and later confirmed through the gene probe method to test for the presence of the zot gene. Consequently, the zot gene was successfully cloned in pCR 2.1 TOPO vector and sequenced, in which the sequences were 99% homologous to the gene bank sequences.
format	Thesis
qualification_level	Master's degree
author	Neela, Vasanthakumari
author_facet	Neela, Vasanthakumari
author_sort	Neela, Vasanthakumari
title	Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus
title_short	Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus
title_full	Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus
title_fullStr	Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus
title_full_unstemmed	Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus
title_sort	molecular and immunological characteristics of vibrio cholerae and vibrio alginolyticus
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Medicine and Health Science
publishDate	2000
url	http://psasir.upm.edu.my/id/eprint/11209/1/FPSK_M_2000_6_A.pdf
_version_	1747811222399483904

Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus

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