Genetic Polymorphisms In Sea Cucumber (Holothuroidea) Using Random Amplified Polymorphic Dna (Rapd)
Random Amplified Polymorphic DNA (RAPD) fingerprinting was used to identify DNA-based genetic polymorphism and estimate genetic variability among 13 species of sea cucumber. It offers a rapid and efficient method for generating a new series of DNA markers in sea cucumber. Seventeen single arbitra...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
2000
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/11210/1/FPSK_M_2000_7.pdf |
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| Summary: | Random Amplified Polymorphic DNA (RAPD) fingerprinting was used to identify
DNA-based genetic polymorphism and estimate genetic variability among 13
species of sea cucumber. It offers a rapid and efficient method for generating a new
series of DNA markers in sea cucumber. Seventeen single arbitrary primers (9,10
and 1 2 nucleotides) with G+C contents 50%-80% were chosen for their utility in
identifying genetic polymorphism in this study. The numbers of amplification
fragments were 3-7 bands per primer that ranged in size from 80 to 1750 nucleotide
base pair. Bands of the amplified DNA was scored and analyzed using the
RAPDistance Package and NTSYS Program. The presence or absence of bands on
the gel detects a DNA polymorphism for inter- and intra-locality studies. There
were 839 types of genotype profiles from 4 locations of sea cucumber with an
average of 43 in Sabah, 44 in Terengganu, 47 in Perak and 49 in Kedah. For each of
the primers, 3 types of genotypes (42%) are the most frequently observed followed
by 38% for 2 genotypes, 1 2% for 4 genotypes and 8% for 1 genotype. |
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