Genetic Polymorphisms In Sea Cucumber (Holothuroidea) Using Random Amplified Polymorphic Dna (Rapd)

Random Amplified Polymorphic DNA (RAPD) fingerprinting was used to identify DNA-based genetic polymorphism and estimate genetic variability among 13 species of sea cucumber. It offers a rapid and efficient method for generating a new series of DNA markers in sea cucumber. Seventeen single arbitra...

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Main Author: Kassim Shaari, Norazila
Format: Thesis
Language:English
English
Published: 2000
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/11210/1/FPSK_M_2000_7_A.pdf
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spelling my-upm-ir.112102011-08-08T05:09:48Z Genetic Polymorphisms In Sea Cucumber (Holothuroidea) Using Random Amplified Polymorphic Dna (Rapd) 2000-11 Kassim Shaari, Norazila Random Amplified Polymorphic DNA (RAPD) fingerprinting was used to identify DNA-based genetic polymorphism and estimate genetic variability among 13 species of sea cucumber. It offers a rapid and efficient method for generating a new series of DNA markers in sea cucumber. Seventeen single arbitrary primers (9,10 and 1 2 nucleotides) with G+C contents 50%-80% were chosen for their utility in identifying genetic polymorphism in this study. The numbers of amplification fragments were 3-7 bands per primer that ranged in size from 80 to 1750 nucleotide base pair. Bands of the amplified DNA was scored and analyzed using the RAPDistance Package and NTSYS Program. The presence or absence of bands on the gel detects a DNA polymorphism for inter- and intra-locality studies. There were 839 types of genotype profiles from 4 locations of sea cucumber with an average of 43 in Sabah, 44 in Terengganu, 47 in Perak and 49 in Kedah. For each of the primers, 3 types of genotypes (42%) are the most frequently observed followed by 38% for 2 genotypes, 1 2% for 4 genotypes and 8% for 1 genotype. 2000-11 Thesis http://psasir.upm.edu.my/id/eprint/11210/ http://psasir.upm.edu.my/id/eprint/11210/1/FPSK_M_2000_7_A.pdf application/pdf en public masters Universiti Putra Malaysia Faculty of Medicine and Health Science English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
topic


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Kassim Shaari, Norazila
Genetic Polymorphisms In Sea Cucumber (Holothuroidea) Using Random Amplified Polymorphic Dna (Rapd)
description Random Amplified Polymorphic DNA (RAPD) fingerprinting was used to identify DNA-based genetic polymorphism and estimate genetic variability among 13 species of sea cucumber. It offers a rapid and efficient method for generating a new series of DNA markers in sea cucumber. Seventeen single arbitrary primers (9,10 and 1 2 nucleotides) with G+C contents 50%-80% were chosen for their utility in identifying genetic polymorphism in this study. The numbers of amplification fragments were 3-7 bands per primer that ranged in size from 80 to 1750 nucleotide base pair. Bands of the amplified DNA was scored and analyzed using the RAPDistance Package and NTSYS Program. The presence or absence of bands on the gel detects a DNA polymorphism for inter- and intra-locality studies. There were 839 types of genotype profiles from 4 locations of sea cucumber with an average of 43 in Sabah, 44 in Terengganu, 47 in Perak and 49 in Kedah. For each of the primers, 3 types of genotypes (42%) are the most frequently observed followed by 38% for 2 genotypes, 1 2% for 4 genotypes and 8% for 1 genotype.
format Thesis
qualification_level Master's degree
author Kassim Shaari, Norazila
author_facet Kassim Shaari, Norazila
author_sort Kassim Shaari, Norazila
title Genetic Polymorphisms In Sea Cucumber (Holothuroidea) Using Random Amplified Polymorphic Dna (Rapd)
title_short Genetic Polymorphisms In Sea Cucumber (Holothuroidea) Using Random Amplified Polymorphic Dna (Rapd)
title_full Genetic Polymorphisms In Sea Cucumber (Holothuroidea) Using Random Amplified Polymorphic Dna (Rapd)
title_fullStr Genetic Polymorphisms In Sea Cucumber (Holothuroidea) Using Random Amplified Polymorphic Dna (Rapd)
title_full_unstemmed Genetic Polymorphisms In Sea Cucumber (Holothuroidea) Using Random Amplified Polymorphic Dna (Rapd)
title_sort genetic polymorphisms in sea cucumber (holothuroidea) using random amplified polymorphic dna (rapd)
granting_institution Universiti Putra Malaysia
granting_department Faculty of Medicine and Health Science
publishDate 2000
url http://psasir.upm.edu.my/id/eprint/11210/1/FPSK_M_2000_7_A.pdf
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