Oncolytic Effect Of Newcastle Disease Virus On The Mcf-7 And Mda-Mb-231 Breast Cancer Cell Lines

The study was conducted to find a new anti-cancer agent for the treatment of breast cancer. The oncolytic effect of Newcastle disease virus (NDV) on MCF-7 and MDAMB- 231 breast cancer cell lines was investigated using various methods. The V 4- UPM and AF2240 strains of NDV were propagated in the...

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Main Author:	Meyyappan, Narayani
Format:	Thesis
Language:	English English
Published:	2003
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Online Access:	http://psasir.upm.edu.my/id/eprint/11262/1/FPSK_M_2003_16_.pdf
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id	my-upm-ir.11262
record_format	uketd_dc
spelling	my-upm-ir.112622024-06-13T03:01:08Z Oncolytic Effect Of Newcastle Disease Virus On The Mcf-7 And Mda-Mb-231 Breast Cancer Cell Lines 2003-08 Meyyappan, Narayani The study was conducted to find a new anti-cancer agent for the treatment of breast cancer. The oncolytic effect of Newcastle disease virus (NDV) on MCF-7 and MDAMB- 231 breast cancer cell lines was investigated using various methods. The V 4- UPM and AF2240 strains of NDV were propagated in the allantoic fluid of 11-daysold embryonated eggs. The allantoic fluid was harvested, purified and stored at - 20°C. The haemagglutination (HA) test was conducted on the purified viruses to ' determine the HA titre of the V4-UPM and AF2240 strains, which were 16 384 and 128 HA units consecutively. The microculture tetrazo1ium (MTT) assay was carried out to detect the cytotoxic effect of the NDV strains on breast cancer cell lines using monolayer and co-culture techniques to determine the IC50 value. The IC50 values for the MCF-7 treated with V4-UPM strain were 4096 and 128 HA units for the monolayer and co-culture, respectively, while, the MDA-MB-231 had an IC50 value of 96 HA units for both techniques. When treated with AF2240 strain, MCF-7 showed IC50 value of 96 and 64 HA units for the monolayer and co-culture methods, respectively. Whereas, the IC50 value for MDA-MB-231 cells was 4 HA units for both techniques. Further studies were done to observe the ultrastructural changes in the treated cells and viewed under an energy filter transmission electron microscope 2003-08 Thesis http://psasir.upm.edu.my/id/eprint/11262/ http://psasir.upm.edu.my/id/eprint/11262/1/FPSK_M_2003_16_.pdf text en public masters Universiti Putra Malaysia Faculty of Medicine and Health Science Othman, Fauziah English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Othman, Fauziah
topic
spellingShingle	Meyyappan, Narayani Oncolytic Effect Of Newcastle Disease Virus On The Mcf-7 And Mda-Mb-231 Breast Cancer Cell Lines
description	The study was conducted to find a new anti-cancer agent for the treatment of breast cancer. The oncolytic effect of Newcastle disease virus (NDV) on MCF-7 and MDAMB- 231 breast cancer cell lines was investigated using various methods. The V 4- UPM and AF2240 strains of NDV were propagated in the allantoic fluid of 11-daysold embryonated eggs. The allantoic fluid was harvested, purified and stored at - 20°C. The haemagglutination (HA) test was conducted on the purified viruses to ' determine the HA titre of the V4-UPM and AF2240 strains, which were 16 384 and 128 HA units consecutively. The microculture tetrazo1ium (MTT) assay was carried out to detect the cytotoxic effect of the NDV strains on breast cancer cell lines using monolayer and co-culture techniques to determine the IC50 value. The IC50 values for the MCF-7 treated with V4-UPM strain were 4096 and 128 HA units for the monolayer and co-culture, respectively, while, the MDA-MB-231 had an IC50 value of 96 HA units for both techniques. When treated with AF2240 strain, MCF-7 showed IC50 value of 96 and 64 HA units for the monolayer and co-culture methods, respectively. Whereas, the IC50 value for MDA-MB-231 cells was 4 HA units for both techniques. Further studies were done to observe the ultrastructural changes in the treated cells and viewed under an energy filter transmission electron microscope
format	Thesis
qualification_level	Master's degree
author	Meyyappan, Narayani
author_facet	Meyyappan, Narayani
author_sort	Meyyappan, Narayani
title	Oncolytic Effect Of Newcastle Disease Virus On The Mcf-7 And Mda-Mb-231 Breast Cancer Cell Lines
title_short	Oncolytic Effect Of Newcastle Disease Virus On The Mcf-7 And Mda-Mb-231 Breast Cancer Cell Lines
title_full	Oncolytic Effect Of Newcastle Disease Virus On The Mcf-7 And Mda-Mb-231 Breast Cancer Cell Lines
title_fullStr	Oncolytic Effect Of Newcastle Disease Virus On The Mcf-7 And Mda-Mb-231 Breast Cancer Cell Lines
title_full_unstemmed	Oncolytic Effect Of Newcastle Disease Virus On The Mcf-7 And Mda-Mb-231 Breast Cancer Cell Lines
title_sort	oncolytic effect of newcastle disease virus on the mcf-7 and mda-mb-231 breast cancer cell lines
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Medicine and Health Science
publishDate	2003
url	http://psasir.upm.edu.my/id/eprint/11262/1/FPSK_M_2003_16_.pdf
_version_	1804888633664077824

Oncolytic Effect Of Newcastle Disease Virus On The Mcf-7 And Mda-Mb-231 Breast Cancer Cell Lines

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