Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment

Clastogenic carcinogen diethylstilbestrol (DES) results in a broad of spectrum of toxic and carcinogenic effects in humans and rodents. Female C57BL/6J wild-type mice (23-25g) and female p53 +/1- hemizygous mice were treated with DES (500 umol/kg) i .p., once daily for 4 days. Control animals wer...

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Main Author:	Salleh, Mohd Nazil
Format:	Thesis
Language:	English English
Published:	2003
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Online Access:	http://psasir.upm.edu.my/id/eprint/11274/1/FPSK_P_2003_2.pdf
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id	my-upm-ir.11274
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spelling	my-upm-ir.112742024-06-13T07:34:40Z Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment 2003-05 Salleh, Mohd Nazil Clastogenic carcinogen diethylstilbestrol (DES) results in a broad of spectrum of toxic and carcinogenic effects in humans and rodents. Female C57BL/6J wild-type mice (23-25g) and female p53 +/1- hemizygous mice were treated with DES (500 umol/kg) i .p., once daily for 4 days. Control animals were treated with the trioctanoin vehicle only. All animals were sacrificed 24 hours after the last dose, and liver, kidney and uterus were harvested and frozen at -80oC . Analysis of differential expression levels of multiple genes involved in apoptosis and cell was performed using cDNA array technology. Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) analysis was carried out in order to verify the expression of these genes. In hepatocytes, murine apoptosis arrays showed three genes: bcl-w, caspase-3 and E2Fl were transcriptionally down-regulated and eight genes: bad, bax, caspase-1, caspase-7, c-myc, p2I, p53 and Rb were up-regulated. In cell-cycle arrays thirteen genes were up-regulated: CDK6, CKK1, Cyclin C, Cyclin D2, Cyclin D3, Cyclin E, Cyclin E2, E2F1, pI6, p19, p21, p57 and Skp1. The greatest change was inp21 gene expression. There was a 3 -fold versus 10-fold increase induction for apoptosis- 2003-05 Thesis http://psasir.upm.edu.my/id/eprint/11274/ http://psasir.upm.edu.my/id/eprint/11274/1/FPSK_P_2003_2.pdf text en public doctoral Universiti Putra Malaysia Faculty of Medicine and Health Science Ismail, Patimah English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Ismail, Patimah
topic
spellingShingle	Salleh, Mohd Nazil Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment
description	Clastogenic carcinogen diethylstilbestrol (DES) results in a broad of spectrum of toxic and carcinogenic effects in humans and rodents. Female C57BL/6J wild-type mice (23-25g) and female p53 +/1- hemizygous mice were treated with DES (500 umol/kg) i .p., once daily for 4 days. Control animals were treated with the trioctanoin vehicle only. All animals were sacrificed 24 hours after the last dose, and liver, kidney and uterus were harvested and frozen at -80oC . Analysis of differential expression levels of multiple genes involved in apoptosis and cell was performed using cDNA array technology. Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) analysis was carried out in order to verify the expression of these genes. In hepatocytes, murine apoptosis arrays showed three genes: bcl-w, caspase-3 and E2Fl were transcriptionally down-regulated and eight genes: bad, bax, caspase-1, caspase-7, c-myc, p2I, p53 and Rb were up-regulated. In cell-cycle arrays thirteen genes were up-regulated: CDK6, CKK1, Cyclin C, Cyclin D2, Cyclin D3, Cyclin E, Cyclin E2, E2F1, pI6, p19, p21, p57 and Skp1. The greatest change was inp21 gene expression. There was a 3 -fold versus 10-fold increase induction for apoptosis-
format	Thesis
qualification_level	Doctorate
author	Salleh, Mohd Nazil
author_facet	Salleh, Mohd Nazil
author_sort	Salleh, Mohd Nazil
title	Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment
title_short	Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment
title_full	Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment
title_fullStr	Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment
title_full_unstemmed	Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment
title_sort	gene expression and metabonomic profilings in p53+/- knockout mice following diethylstilbestrol treatment
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Medicine and Health Science
publishDate	2003
url	http://psasir.upm.edu.my/id/eprint/11274/1/FPSK_P_2003_2.pdf
_version_	1804888635183464448

Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment

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