Molecular cloning and expression analysis of CONSTANS-LIKE 2 in Mucuna bracteate DC. in different tissue and at low temperature
The flowering signals were perceived by angiosperms for the transition from vegetative to reproductive state at their apical tissue. The transition was triggered by environmental conditions and the internal regulation of various genes. Plants are capable of integrating environmental changes such...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2021
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/112971/1/112971.pdf |
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| Summary: | The flowering signals were perceived by angiosperms for the transition from
vegetative to reproductive state at their apical tissue. The transition was triggered
by environmental conditions and the internal regulation of various genes. Plants
are capable of integrating environmental changes such as photoperiod and
temperature into their developmental program. Mucuna bracteata is a legume
originated from North India and is planted as a cover crop in the oil palm and
rubber plantations in Malaysia. This legume is able to grow well locally but unable
to produce flowers. Non-flowering M. bracteata plants may not perceive the
flowering signals to initiate the reproductive phase due to environmental
differences. This study is the first report on the cloning of a putative flowering
gene, CONSTANS-LIKE 2 (COL2) from the leaves of M. bracteata. In this study,
the MbCOL2 sequence with the length of 1335bp was successfully identified and
cloned. MbCOL2 protein (335 amino acid residues) consisted of two BBOX
domains, a CCT domain and a VP motif. The bioinformatic analysis on the
unpublished transcriptome data showed that 13 COL protein members were
present in the M. bracteata genome which had been classified into Groups 1, 2
and 3. To understand the molecular regulation of this gene in locally grown M.
bracteata, the expression analysis of MbCOL2 in different tissues of M.
bracteata seedlings and in response to low temperature was carried out using
real time PCR. MbCOL2 expression in all selected tissues; leaf, shoot apical
meristem and stem of 2-month, 6-month and 12-month old seedlings showed
that MbCOL2 was expressed at the early stage of the plant growth. However, a
higher abundance of MbCOL2 expression was detected in the leaf tissues as
reported previously in other reference species. Meanwhile, the expression of
MbCOL2 was increased by low temperature. However, the actual molecular
mechanism of the flowering initiation of locally grown M. bracteata is still unknown. In the future, identification and expression profiling of various flowering
genes can be performed to further understand the flowering induction in M.
bracteata. |
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