Evaluation of systemic acquired resistance inducer and Phatogenesis Related-gene expression profiling in dieback infected-papaya

Erwinia mallotivora was identified as a pathogen of papaya dieback disease and has become one of the most destructive to the papaya industry. Pathogenesis-related (PR) genes are considered plant defense genes, where they can prevent or reduce the pathogen and insect attacks. Accumulation of PR genes...

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Main Author:	Samsuddin, Normahfuzah Husna
Format:	Thesis
Language:	English English
Published:	2021
Subjects:	Papaya - Diseases and pests Systemic insecticides Gene expression
Online Access:	http://psasir.upm.edu.my/id/eprint/112977/1/112977.pdf
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spelling	my-upm-ir.1129772024-10-23T08:09:48Z Evaluation of systemic acquired resistance inducer and Phatogenesis Related-gene expression profiling in dieback infected-papaya 2021-10 Samsuddin, Normahfuzah Husna Erwinia mallotivora was identified as a pathogen of papaya dieback disease and has become one of the most destructive to the papaya industry. Pathogenesis-related (PR) genes are considered plant defense genes, where they can prevent or reduce the pathogen and insect attacks. Accumulation of PR genes is closely related to developing systemic acquired resistance (SAR) in plants. PR expression can be induced by signaling compounds such as ethylene, salicylic acid (SA), jasmonic acid (JA), mechanical wounding and microbial infection. In this study, salicylic acid (SA) was used as a chemical inducer and their effectiveness in inducing SAR in papaya for enhanced disease resistance to papaya dieback was evaluated. Carica papaya was grown in a glasshouse with Randomized Complete Block Design (RCBD) arrangement. SA treatment with three concentrations (1 mM, 3 mM, 5 mM) and observation of papaya seedling physiology was done to study the effect of SA on plant growth. E. mallotivora inoculations were applied to the papaya seedlings after 1 month of SA applications, and disease assessment was evaluated weekly. The application of SA as a chemical inducer to papaya seedlings showed no negatively effect on Eksotika I seedlings growth. For disease assessment, significant differences were recorded between the control and the treatments. Among 10 PR genes analyzed, only Osmotin, CPBI_13 and CPBI_17were down-regulated for all SA-treatments. The rest (PR1 genes, CPBI_3, CPBI_4 and peroxidase) can be a potential SAR marker in the future. The induced expression of PR genes correlates with the activation of defence responses where they serve as excellent molecular markers of the papaya plant’s defence. From this study, it was shown that salicylic acid is effective in inducing SAR in papaya. The results of this study can improve our understanding of the SAR mechanism and reveal PR genes marker for further research. Papaya - Diseases and pests Systemic insecticides Gene expression 2021-10 Thesis http://psasir.upm.edu.my/id/eprint/112977/ http://psasir.upm.edu.my/id/eprint/112977/1/112977.pdf text en public masters Universiti Putra Malaysia Papaya - Diseases and pests Systemic insecticides Gene expression Shaharuddin, Noor Azmi English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Shaharuddin, Noor Azmi
topic	Papaya - Diseases and pests Systemic insecticides Gene expression
spellingShingle	Papaya - Diseases and pests Systemic insecticides Gene expression Samsuddin, Normahfuzah Husna Evaluation of systemic acquired resistance inducer and Phatogenesis Related-gene expression profiling in dieback infected-papaya
description	Erwinia mallotivora was identified as a pathogen of papaya dieback disease and has become one of the most destructive to the papaya industry. Pathogenesis-related (PR) genes are considered plant defense genes, where they can prevent or reduce the pathogen and insect attacks. Accumulation of PR genes is closely related to developing systemic acquired resistance (SAR) in plants. PR expression can be induced by signaling compounds such as ethylene, salicylic acid (SA), jasmonic acid (JA), mechanical wounding and microbial infection. In this study, salicylic acid (SA) was used as a chemical inducer and their effectiveness in inducing SAR in papaya for enhanced disease resistance to papaya dieback was evaluated. Carica papaya was grown in a glasshouse with Randomized Complete Block Design (RCBD) arrangement. SA treatment with three concentrations (1 mM, 3 mM, 5 mM) and observation of papaya seedling physiology was done to study the effect of SA on plant growth. E. mallotivora inoculations were applied to the papaya seedlings after 1 month of SA applications, and disease assessment was evaluated weekly. The application of SA as a chemical inducer to papaya seedlings showed no negatively effect on Eksotika I seedlings growth. For disease assessment, significant differences were recorded between the control and the treatments. Among 10 PR genes analyzed, only Osmotin, CPBI_13 and CPBI_17were down-regulated for all SA-treatments. The rest (PR1 genes, CPBI_3, CPBI_4 and peroxidase) can be a potential SAR marker in the future. The induced expression of PR genes correlates with the activation of defence responses where they serve as excellent molecular markers of the papaya plant’s defence. From this study, it was shown that salicylic acid is effective in inducing SAR in papaya. The results of this study can improve our understanding of the SAR mechanism and reveal PR genes marker for further research.
format	Thesis
qualification_level	Master's degree
author	Samsuddin, Normahfuzah Husna
author_facet	Samsuddin, Normahfuzah Husna
author_sort	Samsuddin, Normahfuzah Husna
title	Evaluation of systemic acquired resistance inducer and Phatogenesis Related-gene expression profiling in dieback infected-papaya
title_short	Evaluation of systemic acquired resistance inducer and Phatogenesis Related-gene expression profiling in dieback infected-papaya
title_full	Evaluation of systemic acquired resistance inducer and Phatogenesis Related-gene expression profiling in dieback infected-papaya
title_fullStr	Evaluation of systemic acquired resistance inducer and Phatogenesis Related-gene expression profiling in dieback infected-papaya
title_full_unstemmed	Evaluation of systemic acquired resistance inducer and Phatogenesis Related-gene expression profiling in dieback infected-papaya
title_sort	evaluation of systemic acquired resistance inducer and phatogenesis related-gene expression profiling in dieback infected-papaya
granting_institution	Universiti Putra Malaysia
publishDate	2021
url	http://psasir.upm.edu.my/id/eprint/112977/1/112977.pdf
_version_	1818586130796249088

Evaluation of systemic acquired resistance inducer and Phatogenesis Related-gene expression profiling in dieback infected-papaya

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