Pineapple peel as an alternative substrate for production of bacterial nanocellulose

Bacterial nanocellulose (BNC) is a nano fibrillar polymer produced by nanocellulose producing bacteria such as Acetobacter xylinum. The diverse characteristics of BNC such as chemical purity, high mechanical strength, high flexibility, high absorbency, and the ability to create any shape or size...

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Main Author:	Lee, Adriana Connie
Format:	Thesis
Language:	English English
Published:	2022
Subjects:	Pineapple Waste products
Online Access:	http://psasir.upm.edu.my/id/eprint/112998/1/112998.pdf
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id	my-upm-ir.112998
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spelling	my-upm-ir.1129982024-10-23T09:12:50Z Pineapple peel as an alternative substrate for production of bacterial nanocellulose 2022-11 Lee, Adriana Connie Bacterial nanocellulose (BNC) is a nano fibrillar polymer produced by nanocellulose producing bacteria such as Acetobacter xylinum. The diverse characteristics of BNC such as chemical purity, high mechanical strength, high flexibility, high absorbency, and the ability to create any shape or size and others that have attracted a lot of interest. The BNC’s broad usage in food technology, paper, and electronics as well as the architecture in use, is due to its vast number of benefits. However, using commercial glucose as a carbon source in a media such as Hestrin and Schramm (HS) medium in large-scale synthesis of BNC is expensive. As a result, most researchers are looking for alternative substrates from accessible wastes to reduce the cost of BNC production. Thus, this research is aimed to investigate the synthesis of BNC by an isolated bacterial strain using an agro-industrial waste which is the pineapple peel extract. Six bacterial strains namely F8, F5, M1, M6, H7 and H11 were screened and identified for potential BNC producer. The selected bacterial strain was identified as Bacillus cereus MMS1 using 16S rRNA nucleotide sequences. Then, the production of BNC was done by B. cereus MMS1 using pineapple peel extract, while A. xylinum ATCC2376 was acted as a control. The BNC production in this study was attained at 2% (w/v) glucose concentration, 12 days of incubation period and 150 rpm agitation speed which was 5.83 g/L by A. xylinum ATCC2376 in HS medium using commercial glucose as carbon source. Meanwhile, 4.42 g/L and 2 g/L of BNC were produced by B. cereus MMS1 after 12 days of incubation with an initial concentration of 2% (w/v) using commercial glucose and pineapple peel extract, respectively. The fermentation effects of BNC production concludes that the optimum condition to produce BNC by using pineapple peel extract as an alternative substrate is 2% (w/v) carbon source concentration, 12 days of incubation period and 150 rpm agitation. This study showed that agro-industrial wastes such as the pineapple peel may be used in place of costly substrates to produce BNC. Pineapple Waste products 2022-11 Thesis http://psasir.upm.edu.my/id/eprint/112998/ http://psasir.upm.edu.my/id/eprint/112998/1/112998.pdf text en public masters Universiti Putra Malaysia Pineapple Waste products Abd Aziz, Suraini English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Abd Aziz, Suraini
topic	Pineapple Waste products
spellingShingle	Pineapple Waste products Lee, Adriana Connie Pineapple peel as an alternative substrate for production of bacterial nanocellulose
description	Bacterial nanocellulose (BNC) is a nano fibrillar polymer produced by nanocellulose producing bacteria such as Acetobacter xylinum. The diverse characteristics of BNC such as chemical purity, high mechanical strength, high flexibility, high absorbency, and the ability to create any shape or size and others that have attracted a lot of interest. The BNC’s broad usage in food technology, paper, and electronics as well as the architecture in use, is due to its vast number of benefits. However, using commercial glucose as a carbon source in a media such as Hestrin and Schramm (HS) medium in large-scale synthesis of BNC is expensive. As a result, most researchers are looking for alternative substrates from accessible wastes to reduce the cost of BNC production. Thus, this research is aimed to investigate the synthesis of BNC by an isolated bacterial strain using an agro-industrial waste which is the pineapple peel extract. Six bacterial strains namely F8, F5, M1, M6, H7 and H11 were screened and identified for potential BNC producer. The selected bacterial strain was identified as Bacillus cereus MMS1 using 16S rRNA nucleotide sequences. Then, the production of BNC was done by B. cereus MMS1 using pineapple peel extract, while A. xylinum ATCC2376 was acted as a control. The BNC production in this study was attained at 2% (w/v) glucose concentration, 12 days of incubation period and 150 rpm agitation speed which was 5.83 g/L by A. xylinum ATCC2376 in HS medium using commercial glucose as carbon source. Meanwhile, 4.42 g/L and 2 g/L of BNC were produced by B. cereus MMS1 after 12 days of incubation with an initial concentration of 2% (w/v) using commercial glucose and pineapple peel extract, respectively. The fermentation effects of BNC production concludes that the optimum condition to produce BNC by using pineapple peel extract as an alternative substrate is 2% (w/v) carbon source concentration, 12 days of incubation period and 150 rpm agitation. This study showed that agro-industrial wastes such as the pineapple peel may be used in place of costly substrates to produce BNC.
format	Thesis
qualification_level	Master's degree
author	Lee, Adriana Connie
author_facet	Lee, Adriana Connie
author_sort	Lee, Adriana Connie
title	Pineapple peel as an alternative substrate for production of bacterial nanocellulose
title_short	Pineapple peel as an alternative substrate for production of bacterial nanocellulose
title_full	Pineapple peel as an alternative substrate for production of bacterial nanocellulose
title_fullStr	Pineapple peel as an alternative substrate for production of bacterial nanocellulose
title_full_unstemmed	Pineapple peel as an alternative substrate for production of bacterial nanocellulose
title_sort	pineapple peel as an alternative substrate for production of bacterial nanocellulose
granting_institution	Universiti Putra Malaysia
publishDate	2022
url	http://psasir.upm.edu.my/id/eprint/112998/1/112998.pdf
_version_	1818586131564855296

Pineapple peel as an alternative substrate for production of bacterial nanocellulose

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