Bioremediation of canola cooking oil using cold-adapted Antarctic bacterial community from soils

Hydrocarbons can cause pollution to Antarctic terrestrial and aquatic ecosystems, both through accidental release and the discharge of waste cooking oil in greywater. Such pollutants can persist for long periods in cold environments. In this study, using mixed native Antarctic bacterial communities,...

Full description

Saved in:

Bibliographic Details
Main Author:	Mohd Zahri, Khadijah Nabilah
Format:	Thesis
Language:	English English
Published:	2023
Subjects:	Canola oil - Biodegradation Bioremediation Bacteria
Online Access:	http://psasir.upm.edu.my/id/eprint/113036/1/113036.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.113036
record_format	uketd_dc
spelling	my-upm-ir.1130362024-10-24T07:39:35Z Bioremediation of canola cooking oil using cold-adapted Antarctic bacterial community from soils 2023-02 Mohd Zahri, Khadijah Nabilah Hydrocarbons can cause pollution to Antarctic terrestrial and aquatic ecosystems, both through accidental release and the discharge of waste cooking oil in greywater. Such pollutants can persist for long periods in cold environments. In this study, using mixed native Antarctic bacterial communities, several environmental factors influencing biodegradation of waste canola oil (WCO) and canola oil (CO) were optimised using established one-factor-at-a-time (OFAT) and response surface methodology (RSM) approaches. Secondary mathematical equations were chosen for kinetic analyses and the effect of co-contaminant heavy metals on WCO biodegradation was discussed. The toxicity of different heavy metals in 1 ppm of concentration to the WCO-degrading bacteria was evaluated and further analysed using half-maximal inhibition concentration (IC50) and effective concentration (EC50) tests. Next, biosurfactant production was optimised using RSM after preliminary screening processes. The bacterial community was then investigated through metagenomics analysis and so with lipase-producing bacteria was identified using Sanger sequencing. As for the result, an Antarctic soil bacterial community (reference BS14) was confirmed to biodegrade canola oil. Using OFAT, the most effective microbial community examined was able to degrade 94.42% and 86.83% (from an initial concentration of 0.5% (v/v)) of WCO and CO, respectively, within 7 days. Using RSM, 94.99% and 79.77% degradation of WCO and CO was achieved in 6 days. Mathematical modelling demonstrated that the best-fitting model was the Haldane model for both WCO and CO degradation. Kinetic parameters including the maximum degradation rate (μmax) were obtained, which were 0.365 and 0.307 min−1 for WCO and CO degradation, respectively. As for heavy metal evaluation, the IC50 values of Ag and Hg for WCO degradation were 0.47 and 0.54 ppm, respectively. Meanwhile, Cr, As and Pb were well-tolerated and induced bacterial growth and WCO degradation, resulting in the EC50 values of 3.00, 23.80, and 28.98 ppm, respectively. Next, all preliminary screenings for biosurfactants were positive, where biosurfactant concentrations produced up to 13.44 and 14.06 mg/mL in the presence of WCO and CO, respectively, after optimisation. The bacterial community in non-treated media were originated from Proteobacteria and Firmicutes families. High proportions of bacterial families in WCO and CO treated media were Pseudomonadaceae (98.27%), followed by Carnobacteriaceae (1.70%). Among all the bacterial strains identified in the metagenomic analysis, it was confirmed that a group of Pseudomonas and Carnobacterium strains were responsible for biodegrading WCO and CO. Hence, this study offers a novel insight into the potential of the BS14 Antarctic bacterial community for canola oil bioremediation in Antarctica due to its ability to degrade WCO and CO effectively and produce biosurfactant at the same time. Plus, the BS14 community was able to tolerate heavy metals while biodegrading WCO in low-temperature conditions, which is a crucial aspect in biodegrading oil due to the co-contamination of oil and heavy metals that can occur simultaneously, and at the same time it can be applied in heavy metal-contaminated areas. Canola oil - Biodegradation Bioremediation Bacteria 2023-02 Thesis http://psasir.upm.edu.my/id/eprint/113036/ http://psasir.upm.edu.my/id/eprint/113036/1/113036.pdf text en public doctoral Universiti Putra Malaysia Canola oil - Biodegradation Bioremediation Bacteria Ahmad, Siti Aqlima English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Ahmad, Siti Aqlima
topic	Canola oil - Biodegradation Bioremediation Bacteria
spellingShingle	Canola oil - Biodegradation Bioremediation Bacteria Mohd Zahri, Khadijah Nabilah Bioremediation of canola cooking oil using cold-adapted Antarctic bacterial community from soils
description	Hydrocarbons can cause pollution to Antarctic terrestrial and aquatic ecosystems, both through accidental release and the discharge of waste cooking oil in greywater. Such pollutants can persist for long periods in cold environments. In this study, using mixed native Antarctic bacterial communities, several environmental factors influencing biodegradation of waste canola oil (WCO) and canola oil (CO) were optimised using established one-factor-at-a-time (OFAT) and response surface methodology (RSM) approaches. Secondary mathematical equations were chosen for kinetic analyses and the effect of co-contaminant heavy metals on WCO biodegradation was discussed. The toxicity of different heavy metals in 1 ppm of concentration to the WCO-degrading bacteria was evaluated and further analysed using half-maximal inhibition concentration (IC50) and effective concentration (EC50) tests. Next, biosurfactant production was optimised using RSM after preliminary screening processes. The bacterial community was then investigated through metagenomics analysis and so with lipase-producing bacteria was identified using Sanger sequencing. As for the result, an Antarctic soil bacterial community (reference BS14) was confirmed to biodegrade canola oil. Using OFAT, the most effective microbial community examined was able to degrade 94.42% and 86.83% (from an initial concentration of 0.5% (v/v)) of WCO and CO, respectively, within 7 days. Using RSM, 94.99% and 79.77% degradation of WCO and CO was achieved in 6 days. Mathematical modelling demonstrated that the best-fitting model was the Haldane model for both WCO and CO degradation. Kinetic parameters including the maximum degradation rate (μmax) were obtained, which were 0.365 and 0.307 min−1 for WCO and CO degradation, respectively. As for heavy metal evaluation, the IC50 values of Ag and Hg for WCO degradation were 0.47 and 0.54 ppm, respectively. Meanwhile, Cr, As and Pb were well-tolerated and induced bacterial growth and WCO degradation, resulting in the EC50 values of 3.00, 23.80, and 28.98 ppm, respectively. Next, all preliminary screenings for biosurfactants were positive, where biosurfactant concentrations produced up to 13.44 and 14.06 mg/mL in the presence of WCO and CO, respectively, after optimisation. The bacterial community in non-treated media were originated from Proteobacteria and Firmicutes families. High proportions of bacterial families in WCO and CO treated media were Pseudomonadaceae (98.27%), followed by Carnobacteriaceae (1.70%). Among all the bacterial strains identified in the metagenomic analysis, it was confirmed that a group of Pseudomonas and Carnobacterium strains were responsible for biodegrading WCO and CO. Hence, this study offers a novel insight into the potential of the BS14 Antarctic bacterial community for canola oil bioremediation in Antarctica due to its ability to degrade WCO and CO effectively and produce biosurfactant at the same time. Plus, the BS14 community was able to tolerate heavy metals while biodegrading WCO in low-temperature conditions, which is a crucial aspect in biodegrading oil due to the co-contamination of oil and heavy metals that can occur simultaneously, and at the same time it can be applied in heavy metal-contaminated areas.
format	Thesis
qualification_level	Doctorate
author	Mohd Zahri, Khadijah Nabilah
author_facet	Mohd Zahri, Khadijah Nabilah
author_sort	Mohd Zahri, Khadijah Nabilah
title	Bioremediation of canola cooking oil using cold-adapted Antarctic bacterial community from soils
title_short	Bioremediation of canola cooking oil using cold-adapted Antarctic bacterial community from soils
title_full	Bioremediation of canola cooking oil using cold-adapted Antarctic bacterial community from soils
title_fullStr	Bioremediation of canola cooking oil using cold-adapted Antarctic bacterial community from soils
title_full_unstemmed	Bioremediation of canola cooking oil using cold-adapted Antarctic bacterial community from soils
title_sort	bioremediation of canola cooking oil using cold-adapted antarctic bacterial community from soils
granting_institution	Universiti Putra Malaysia
publishDate	2023
url	http://psasir.upm.edu.my/id/eprint/113036/1/113036.pdf
_version_	1818586133951414272

Bioremediation of canola cooking oil using cold-adapted Antarctic bacterial community from soils

Similar Items