Sequencing, Cloning And Expression Of The Newcastle Disease Virus Fusion Protein Gene Of Strain Af2240
Newcastle disease is a highly contagious viral disease of poultry and has greater than 95 % morbidity and mortality. The etiological agent, Newcastle disease virus (NOV) , is a member of the family Paramyxoviridae and the genus Rubulavirus. Immune responses to the fusion protein (F) antigen of NO...
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1999
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my-upm-ir.113972012-08-14T05:03:07Z Sequencing, Cloning And Expression Of The Newcastle Disease Virus Fusion Protein Gene Of Strain Af2240 1999-08 Mohammed, Omeima Salih Newcastle disease is a highly contagious viral disease of poultry and has greater than 95 % morbidity and mortality. The etiological agent, Newcastle disease virus (NOV) , is a member of the family Paramyxoviridae and the genus Rubulavirus. Immune responses to the fusion protein (F) antigen of NOV were demonstrated to play an important role in the prevention of infection. Accordingly, the F gene of a Malaysian heat-resistant NOV velogenic-viscerotropic strain A F2240 was amplified and cloned into pCR™2 . 1 vector. Its sequence was determined by sequencing of the cloned F gene RT-PCR products. It contains 1791 nucleotides and encodes a large ORF of 553 amino acids. The deduced amino acid sequences of the Fo precursor protein has a pair of dibasic residues with an intervening glutamine (Q) at the proteolytic site, three hydrophobic regions and six asparginine glycosylation sites. The cleavage-activation site as well as part of the fusion-inducing sequence were compared among a series of virulent and avirulent NOV strains using multiple Newcastle disease virus. 1999-08 Thesis http://psasir.upm.edu.my/id/eprint/11397/ http://psasir.upm.edu.my/id/eprint/11397/1/FSAS_1999_43_A.pdf application/pdf en public phd doctoral Universiti Putra Malaysia Newcastle disease virus. Faculty of Environmental studies English |
| institution |
Universiti Putra Malaysia |
| collection |
PSAS Institutional Repository |
| language |
English English |
| topic |
Newcastle disease virus. |
| spellingShingle |
Newcastle disease virus. Mohammed, Omeima Salih Sequencing, Cloning And Expression Of The Newcastle Disease Virus Fusion Protein Gene Of Strain Af2240 |
| description |
Newcastle disease is a highly contagious viral disease of poultry and has
greater than 95 % morbidity and mortality. The etiological agent, Newcastle disease
virus (NOV) , is a member of the family Paramyxoviridae and the genus Rubulavirus.
Immune responses to the fusion protein (F) antigen of NOV were demonstrated to
play an important role in the prevention of infection. Accordingly, the F gene of a
Malaysian heat-resistant NOV velogenic-viscerotropic strain A F2240 was amplified
and cloned into pCR™2 . 1 vector. Its sequence was determined by sequencing of
the cloned F gene RT-PCR products. It contains 1791 nucleotides and encodes a
large ORF of 553 amino acids. The deduced amino acid sequences of the Fo
precursor protein has a pair of dibasic residues with an intervening glutamine (Q) at
the proteolytic site, three hydrophobic regions and six asparginine glycosylation
sites. The cleavage-activation site as well as part of the fusion-inducing sequence
were compared among a series of virulent and avirulent NOV strains using multiple |
| format |
Thesis |
| qualification_name |
Doctor of Philosophy (PhD.) |
| qualification_level |
Doctorate |
| author |
Mohammed, Omeima Salih |
| author_facet |
Mohammed, Omeima Salih |
| author_sort |
Mohammed, Omeima Salih |
| title |
Sequencing, Cloning And Expression Of The Newcastle Disease Virus Fusion Protein Gene Of Strain Af2240
|
| title_short |
Sequencing, Cloning And Expression Of The Newcastle Disease Virus Fusion Protein Gene Of Strain Af2240
|
| title_full |
Sequencing, Cloning And Expression Of The Newcastle Disease Virus Fusion Protein Gene Of Strain Af2240
|
| title_fullStr |
Sequencing, Cloning And Expression Of The Newcastle Disease Virus Fusion Protein Gene Of Strain Af2240
|
| title_full_unstemmed |
Sequencing, Cloning And Expression Of The Newcastle Disease Virus Fusion Protein Gene Of Strain Af2240
|
| title_sort |
sequencing, cloning and expression of the newcastle disease virus fusion protein gene of strain af2240 |
| granting_institution |
Universiti Putra Malaysia |
| granting_department |
Faculty of Environmental studies |
| publishDate |
1999 |
| url |
http://psasir.upm.edu.my/id/eprint/11397/1/FSAS_1999_43_A.pdf |
| _version_ |
1747811239222837248 |