Evaluation and characterization of spider venom for controlling blow fly, Chrysomya megacephala fabricius (Diptera: Calliphoridae)

Blow fly (Chrysomya megacephala) is a well-known cosmopolitan pest in most poultry farms. In Malaysia, an unpublished data of spider species Leucauge sp. and Pardosa sp. have shown insecticidal activity against the larvae of Spodoptera litura and Spodoptera exigua. However, their potential use in...

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Main Author:	Saw, Siew Fen
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Insects - Venom Blowflies
Online Access:	http://psasir.upm.edu.my/id/eprint/114008/1/114008.pdf
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spelling	my-upm-ir.1140082024-12-05T01:26:10Z Evaluation and characterization of spider venom for controlling blow fly, Chrysomya megacephala fabricius (Diptera: Calliphoridae) 2018-05 Saw, Siew Fen Blow fly (Chrysomya megacephala) is a well-known cosmopolitan pest in most poultry farms. In Malaysia, an unpublished data of spider species Leucauge sp. and Pardosa sp. have shown insecticidal activity against the larvae of Spodoptera litura and Spodoptera exigua. However, their potential use in controlling C. megacephala is unknown. Hence, the objectives of this study were to evaluate the insecticidal activity of spider venom(s) against C. megacephala as well as to characterize and evaluate the spider toxin(s) with potential insecticidal activity. The spider venoms were extracted from the field collected spiders, precipitated and fractionated by HPLC. The crude venom of Pardosa sp. did not induce mortality in maggots but the crude venom of Leucauge sp. had caused 100% mortality towards the maggots. The lethal dosage (LD50) value of both crude venom and partial purified venom of Leucauge sp. was 38.92 and 33.19 μg/g, respectively. The SDSPAGE results had revealed that protein bands of supernatant of partial purified venom contained low molecular weight proteins (less than 11 kDa). Three HPLC purified fractions namely F7, F8 and F9 showed positive response to maggots with LD50 values of 1.61 μg/g, 2.33μg/g and 2.12 μg/g, respectively. The Q-TOF mass spectrometry analysis had confirmed these fractions fall into the class of disulfiderich neurotoxins and linear cytolytic peptides (1-10 kDa), within the toxicity range of the reported Ctenitoxin from Phoneutria nigriventer on Musca domestica. The potency test had confirmed negative response of Leucauge sp. venom towards Artemia salina which is a common organism used for toxicity test. Insects - Venom Blowflies 2018-05 Thesis http://psasir.upm.edu.my/id/eprint/114008/ http://psasir.upm.edu.my/id/eprint/114008/1/114008.pdf text en public http://ethesis.upm.edu.my/id/eprint/18062 masters Universiti Putra Malaysia Insects - Venom Blowflies Lau, Wei Hong
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Lau, Wei Hong
topic	Insects - Venom Blowflies
spellingShingle	Insects - Venom Blowflies Saw, Siew Fen Evaluation and characterization of spider venom for controlling blow fly, Chrysomya megacephala fabricius (Diptera: Calliphoridae)
description	Blow fly (Chrysomya megacephala) is a well-known cosmopolitan pest in most poultry farms. In Malaysia, an unpublished data of spider species Leucauge sp. and Pardosa sp. have shown insecticidal activity against the larvae of Spodoptera litura and Spodoptera exigua. However, their potential use in controlling C. megacephala is unknown. Hence, the objectives of this study were to evaluate the insecticidal activity of spider venom(s) against C. megacephala as well as to characterize and evaluate the spider toxin(s) with potential insecticidal activity. The spider venoms were extracted from the field collected spiders, precipitated and fractionated by HPLC. The crude venom of Pardosa sp. did not induce mortality in maggots but the crude venom of Leucauge sp. had caused 100% mortality towards the maggots. The lethal dosage (LD50) value of both crude venom and partial purified venom of Leucauge sp. was 38.92 and 33.19 μg/g, respectively. The SDSPAGE results had revealed that protein bands of supernatant of partial purified venom contained low molecular weight proteins (less than 11 kDa). Three HPLC purified fractions namely F7, F8 and F9 showed positive response to maggots with LD50 values of 1.61 μg/g, 2.33μg/g and 2.12 μg/g, respectively. The Q-TOF mass spectrometry analysis had confirmed these fractions fall into the class of disulfiderich neurotoxins and linear cytolytic peptides (1-10 kDa), within the toxicity range of the reported Ctenitoxin from Phoneutria nigriventer on Musca domestica. The potency test had confirmed negative response of Leucauge sp. venom towards Artemia salina which is a common organism used for toxicity test.
format	Thesis
qualification_level	Master's degree
author	Saw, Siew Fen
author_facet	Saw, Siew Fen
author_sort	Saw, Siew Fen
title	Evaluation and characterization of spider venom for controlling blow fly, Chrysomya megacephala fabricius (Diptera: Calliphoridae)
title_short	Evaluation and characterization of spider venom for controlling blow fly, Chrysomya megacephala fabricius (Diptera: Calliphoridae)
title_full	Evaluation and characterization of spider venom for controlling blow fly, Chrysomya megacephala fabricius (Diptera: Calliphoridae)
title_fullStr	Evaluation and characterization of spider venom for controlling blow fly, Chrysomya megacephala fabricius (Diptera: Calliphoridae)
title_full_unstemmed	Evaluation and characterization of spider venom for controlling blow fly, Chrysomya megacephala fabricius (Diptera: Calliphoridae)
title_sort	evaluation and characterization of spider venom for controlling blow fly, chrysomya megacephala fabricius (diptera: calliphoridae)
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/114008/1/114008.pdf
_version_	1818586180618289152

Evaluation and characterization of spider venom for controlling blow fly, Chrysomya megacephala fabricius (Diptera: Calliphoridae)

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