Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl
Cloning of alkaline protease gene from a thermophilic bacteria, Bacillus stearothermophilus Fl (BSF1) was done by PCR cloning method. Genomic DNA of BSFI was extracted and the highest concentration obtained was 0.46 ug/uL and with a purity of 2.0. Three sets of primers were used including RM5 (5&...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English English |
| Published: |
2000
|
| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/11436/1/FSAS_2000_25.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Cloning of alkaline protease gene from a thermophilic bacteria, Bacillus
stearothermophilus Fl (BSF1) was done by PCR cloning method. Genomic DNA
of BSFI was extracted and the highest concentration obtained was 0.46 ug/uL and
with a purity of 2.0. Three sets of primers were used including RM5 (5'-CA(CT)
GG(ACGT) ACC AA(CT) GTG GC(CGT) GG-3) and RM6 (5'-(ACG)GG GGT
(ACG)GC CAT GGA (CGT)CC-3', F0R900 (5-GCA TGC TAC GAT TAA ATA
TC-3) and REV900 (5'-CGG CAA TAT CAC ITA GAG TAC C-3') and FOR900
(5'-GCA TGC TAC GAT TAA ATA TC-3') and REV1 591 (5-TGC AGC AGA
AAG AAG GAA-3') which resuhed in amplification of 500-bp, 900-bp and 1 500-
bp products, respectively. Southern blotting analysis suggested that both 500-bp
and 900-bp fragment were present within the 1 500-bp fragment. E. coli TOPIOF |
|---|