Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl

Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl

Cloning of alkaline protease gene from a thermophilic bacteria, Bacillus stearothermophilus Fl (BSF1) was done by PCR cloning method. Genomic DNA of BSFI was extracted and the highest concentration obtained was 0.46 ug/uL and with a purity of 2.0. Three sets of primers were used including RM5 (5&...

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Main Author: Ab.Hamid, Suhaili
Format: Thesis
Language:English
English
Published: 2000
Subjects:
Molecular cloning
Online Access:http://psasir.upm.edu.my/id/eprint/11436/1/FSAS_2000_25.pdf
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spelling my-upm-ir.114362024-06-19T07:26:35Z Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl 2000-06 Ab.Hamid, Suhaili Cloning of alkaline protease gene from a thermophilic bacteria, Bacillus stearothermophilus Fl (BSF1) was done by PCR cloning method. Genomic DNA of BSFI was extracted and the highest concentration obtained was 0.46 ug/uL and with a purity of 2.0. Three sets of primers were used including RM5 (5'-CA(CT) GG(ACGT) ACC AA(CT) GTG GC(CGT) GG-3) and RM6 (5'-(ACG)GG GGT (ACG)GC CAT GGA (CGT)CC-3', F0R900 (5-GCA TGC TAC GAT TAA ATA TC-3) and REV900 (5'-CGG CAA TAT CAC ITA GAG TAC C-3') and FOR900 (5'-GCA TGC TAC GAT TAA ATA TC-3') and REV1 591 (5-TGC AGC AGA AAG AAG GAA-3') which resuhed in amplification of 500-bp, 900-bp and 1 500- bp products, respectively. Southern blotting analysis suggested that both 500-bp and 900-bp fragment were present within the 1 500-bp fragment. E. coli TOPIOF Molecular cloning 2000-06 Thesis http://psasir.upm.edu.my/id/eprint/11436/ http://psasir.upm.edu.my/id/eprint/11436/1/FSAS_2000_25.pdf text en public masters Universiti Putra Malaysia Molecular cloning Faculty of Environmental studies Ab. Razak, Nyonya English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
advisor Ab. Razak, Nyonya
topic Molecular cloning
spellingShingle Molecular cloning


Ab.Hamid, Suhaili
Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl
description Cloning of alkaline protease gene from a thermophilic bacteria, Bacillus stearothermophilus Fl (BSF1) was done by PCR cloning method. Genomic DNA of BSFI was extracted and the highest concentration obtained was 0.46 ug/uL and with a purity of 2.0. Three sets of primers were used including RM5 (5'-CA(CT) GG(ACGT) ACC AA(CT) GTG GC(CGT) GG-3) and RM6 (5'-(ACG)GG GGT (ACG)GC CAT GGA (CGT)CC-3', F0R900 (5-GCA TGC TAC GAT TAA ATA TC-3) and REV900 (5'-CGG CAA TAT CAC ITA GAG TAC C-3') and FOR900 (5'-GCA TGC TAC GAT TAA ATA TC-3') and REV1 591 (5-TGC AGC AGA AAG AAG GAA-3') which resuhed in amplification of 500-bp, 900-bp and 1 500- bp products, respectively. Southern blotting analysis suggested that both 500-bp and 900-bp fragment were present within the 1 500-bp fragment. E. coli TOPIOF
format Thesis
qualification_level Master's degree
author Ab.Hamid, Suhaili
author_facet Ab.Hamid, Suhaili
author_sort Ab.Hamid, Suhaili
title Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl
title_short Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl
title_full Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl
title_fullStr Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl
title_full_unstemmed Cloning And Expression Of Alkaline Protease Gene From Bacillus Stearothermophilus Strain Fl
title_sort cloning and expression of alkaline protease gene from bacillus stearothermophilus strain fl
granting_institution Universiti Putra Malaysia
granting_department Faculty of Environmental studies
publishDate 2000
url http://psasir.upm.edu.my/id/eprint/11436/1/FSAS_2000_25.pdf
_version_ 1804888638421467136

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