Infectious Bovine Rhinotracheitis Virus of Cattle and Buffaloes

Infectious bovine rhinotracheitis virus infection in cattle has been reported throughout the world. The study of infectious bovine rhinotracheitis in buffalo was very limited. There was no report on infectious bovine rhinotracheitis in cattle and buffalo in Malaysia. A serological study on the prev...

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Main Author:	Saw, Plei Saw
Format:	Thesis
Language:	English English
Published:	1983
Subjects:	Infectious bovine rhinotracheitis
Online Access:	http://psasir.upm.edu.my/id/eprint/11471/1/FPV_1983_4_A.pdf
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id	my-upm-ir.11471
record_format	uketd_dc
spelling	my-upm-ir.114712011-06-21T04:26:18Z Infectious Bovine Rhinotracheitis Virus of Cattle and Buffaloes 1983-06 Saw, Plei Saw Infectious bovine rhinotracheitis virus infection in cattle has been reported throughout the world. The study of infectious bovine rhinotracheitis in buffalo was very limited. There was no report on infectious bovine rhinotracheitis in cattle and buffalo in Malaysia. A serological study on the prevalence of infectious bovine rhinotracheitis in cattle and buffalo in 10 states of Peninsular Malaysia showed that 52.52 per cent of the cattle and 65.07 per cent of the buffaloes had neutralizing antibodies to infectious bovine rhinotracheitis virus. Neutralizing antibodies were detected in 532 out of the 1013 serum samples from cattle and in 298 out of the 458 serum samples from buffaloes. The titre of the serum neutralizing antibodies ranged from 1:4 to 1:256. Four cattle and four buffaloes with neutralizing antibodies to infectious bovine rhinotracheitis virus were treated with dexamethazone at 0.1mg. per kilogram of body weight for 7 consecutive days. Treatment of these animals with dexamethazone resulted in shedding of virus. Viruses were isolated from nasal cavities and vaginas of the cattle and buffaloes in bovine embryonic kidney cells. The isolates were identified as infectious bovine rhinotracheitis virus by virus neutralizationtest. The viruses isolated from nasal cavity and vaginal mucosa of buffaloes were designated UPM BB 1 and UPM BB 2 respectively while the viruses isolated from nasal cavity and vaginal mucos a of cattle were designated UPM Be 1 and UPM Be 2 respectively. The 4 virus isolates had infective titres ranging from 10^6.8 to 10^7.8 Pfu per mL. The four infectious bovine rhinotracheitis virus isolates were then subjected to 3 cycles of plaque purification. The cloned infectious bovine rhino tracheitis viruses were further purified by rate zonal ultracentrifugat ion in 10 to 40 percent potassium tartrate. Examination of purified viruses under the electron microscope revealed virus morphology similar to that of herpes virus. The four purified virus preparations were also identified as infectious bovine rhinotracheitis virus by immune electron microscopy. Infectious bovine rhinotracheitis 1983-06 Thesis http://psasir.upm.edu.my/id/eprint/11471/ http://psasir.upm.edu.my/id/eprint/11471/1/FPV_1983_4_A.pdf application/pdf en public phd doctoral Universiti Pertanian Malaysia Infectious bovine rhinotracheitis Faculty of Veterinary Medicine English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Infectious bovine rhinotracheitis
spellingShingle	Infectious bovine rhinotracheitis Saw, Plei Saw Infectious Bovine Rhinotracheitis Virus of Cattle and Buffaloes
description	Infectious bovine rhinotracheitis virus infection in cattle has been reported throughout the world. The study of infectious bovine rhinotracheitis in buffalo was very limited. There was no report on infectious bovine rhinotracheitis in cattle and buffalo in Malaysia. A serological study on the prevalence of infectious bovine rhinotracheitis in cattle and buffalo in 10 states of Peninsular Malaysia showed that 52.52 per cent of the cattle and 65.07 per cent of the buffaloes had neutralizing antibodies to infectious bovine rhinotracheitis virus. Neutralizing antibodies were detected in 532 out of the 1013 serum samples from cattle and in 298 out of the 458 serum samples from buffaloes. The titre of the serum neutralizing antibodies ranged from 1:4 to 1:256. Four cattle and four buffaloes with neutralizing antibodies to infectious bovine rhinotracheitis virus were treated with dexamethazone at 0.1mg. per kilogram of body weight for 7 consecutive days. Treatment of these animals with dexamethazone resulted in shedding of virus. Viruses were isolated from nasal cavities and vaginas of the cattle and buffaloes in bovine embryonic kidney cells. The isolates were identified as infectious bovine rhinotracheitis virus by virus neutralizationtest. The viruses isolated from nasal cavity and vaginal mucosa of buffaloes were designated UPM BB 1 and UPM BB 2 respectively while the viruses isolated from nasal cavity and vaginal mucos a of cattle were designated UPM Be 1 and UPM Be 2 respectively. The 4 virus isolates had infective titres ranging from 10^6.8 to 10^7.8 Pfu per mL. The four infectious bovine rhinotracheitis virus isolates were then subjected to 3 cycles of plaque purification. The cloned infectious bovine rhino tracheitis viruses were further purified by rate zonal ultracentrifugat ion in 10 to 40 percent potassium tartrate. Examination of purified viruses under the electron microscope revealed virus morphology similar to that of herpes virus. The four purified virus preparations were also identified as infectious bovine rhinotracheitis virus by immune electron microscopy.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Saw, Plei Saw
author_facet	Saw, Plei Saw
author_sort	Saw, Plei Saw
title	Infectious Bovine Rhinotracheitis Virus of Cattle and Buffaloes
title_short	Infectious Bovine Rhinotracheitis Virus of Cattle and Buffaloes
title_full	Infectious Bovine Rhinotracheitis Virus of Cattle and Buffaloes
title_fullStr	Infectious Bovine Rhinotracheitis Virus of Cattle and Buffaloes
title_full_unstemmed	Infectious Bovine Rhinotracheitis Virus of Cattle and Buffaloes
title_sort	infectious bovine rhinotracheitis virus of cattle and buffaloes
granting_institution	Universiti Pertanian Malaysia
granting_department	Faculty of Veterinary Medicine
publishDate	1983
url	http://psasir.upm.edu.my/id/eprint/11471/1/FPV_1983_4_A.pdf
_version_	1747811242643292160

Infectious Bovine Rhinotracheitis Virus of Cattle and Buffaloes

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