Determination Of The Nucleotide Sequence Of Recombinant Plasmid Pbx6 Containing A Xylanase Gene From Fibrobacter Succinogenes S 85
pBX6 is a recombinant plasmid containing a 3 kb fragment of insert D NA from Fibrobacter succinogenes S 85 genomic DNA which encodes xylanase activity. The recombinant plasmid pBX6 has shown xylanase activity when grown on RBB-xylan plates supplemented with ampicillin. Restriction mapping of the...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English English |
| Published: |
2001
|
| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/11506/1/FSAS_2001_39.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | pBX6 is a recombinant plasmid containing a 3 kb fragment of insert D NA
from Fibrobacter succinogenes S 85 genomic DNA which encodes
xylanase activity. The recombinant plasmid pBX6 has shown xylanase
activity when grown on RBB-xylan plates supplemented with ampicillin.
Restriction mapping of the plasmid established the presence of single sites
for EcoRI, Kpnl, Apal, Saci, EcoRV and Pstf. This work was focussed on
nucleotide sequence determination of recombinant plasmid pBX6
containing a xyJanase gene from F. succinogenes S 85. The primer
walking strategy was performed to complete manual sequencing, following
the protocol cycle sequencing of Sanger dideoxy chain termination. Custom
synthesis primer was designed and constructed from 3' end of the
preceding sequence on both strands. |
|---|