Determination Of The Nucleotide Sequence Of Recombinant Plasmid Pbx6 Containing A Xylanase Gene From Fibrobacter Succinogenes S 85

pBX6 is a recombinant plasmid containing a 3 kb fragment of insert D NA from Fibrobacter succinogenes S 85 genomic DNA which encodes xylanase activity. The recombinant plasmid pBX6 has shown xylanase activity when grown on RBB-xylan plates supplemented with ampicillin. Restriction mapping of the...

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Main Author:	Suryadi, Yadi
Format:	Thesis
Language:	English English
Published:	2001
Subjects:	Genetic recombination - Research
Online Access:	http://psasir.upm.edu.my/id/eprint/11506/1/FSAS_2001_39.pdf
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id	my-upm-ir.11506
record_format	uketd_dc
spelling	my-upm-ir.115062024-06-20T04:19:24Z Determination Of The Nucleotide Sequence Of Recombinant Plasmid Pbx6 Containing A Xylanase Gene From Fibrobacter Succinogenes S 85 2001-10 Suryadi, Yadi pBX6 is a recombinant plasmid containing a 3 kb fragment of insert D NA from Fibrobacter succinogenes S 85 genomic DNA which encodes xylanase activity. The recombinant plasmid pBX6 has shown xylanase activity when grown on RBB-xylan plates supplemented with ampicillin. Restriction mapping of the plasmid established the presence of single sites for EcoRI, Kpnl, Apal, Saci, EcoRV and Pstf. This work was focussed on nucleotide sequence determination of recombinant plasmid pBX6 containing a xyJanase gene from F. succinogenes S 85. The primer walking strategy was performed to complete manual sequencing, following the protocol cycle sequencing of Sanger dideoxy chain termination. Custom synthesis primer was designed and constructed from 3' end of the preceding sequence on both strands. Genetic recombination - Research 2001-10 Thesis http://psasir.upm.edu.my/id/eprint/11506/ http://psasir.upm.edu.my/id/eprint/11506/1/FSAS_2001_39.pdf text en public masters Universiti Putra Malaysia Genetic recombination - Research Faculty of Environmental studies S ipat, Abdullah English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	S ipat, Abdullah
topic	Genetic recombination - Research
spellingShingle	Genetic recombination - Research Suryadi, Yadi Determination Of The Nucleotide Sequence Of Recombinant Plasmid Pbx6 Containing A Xylanase Gene From Fibrobacter Succinogenes S 85
description	pBX6 is a recombinant plasmid containing a 3 kb fragment of insert D NA from Fibrobacter succinogenes S 85 genomic DNA which encodes xylanase activity. The recombinant plasmid pBX6 has shown xylanase activity when grown on RBB-xylan plates supplemented with ampicillin. Restriction mapping of the plasmid established the presence of single sites for EcoRI, Kpnl, Apal, Saci, EcoRV and Pstf. This work was focussed on nucleotide sequence determination of recombinant plasmid pBX6 containing a xyJanase gene from F. succinogenes S 85. The primer walking strategy was performed to complete manual sequencing, following the protocol cycle sequencing of Sanger dideoxy chain termination. Custom synthesis primer was designed and constructed from 3' end of the preceding sequence on both strands.
format	Thesis
qualification_level	Master's degree
author	Suryadi, Yadi
author_facet	Suryadi, Yadi
author_sort	Suryadi, Yadi
title	Determination Of The Nucleotide Sequence Of Recombinant Plasmid Pbx6 Containing A Xylanase Gene From Fibrobacter Succinogenes S 85
title_short	Determination Of The Nucleotide Sequence Of Recombinant Plasmid Pbx6 Containing A Xylanase Gene From Fibrobacter Succinogenes S 85
title_full	Determination Of The Nucleotide Sequence Of Recombinant Plasmid Pbx6 Containing A Xylanase Gene From Fibrobacter Succinogenes S 85
title_fullStr	Determination Of The Nucleotide Sequence Of Recombinant Plasmid Pbx6 Containing A Xylanase Gene From Fibrobacter Succinogenes S 85
title_full_unstemmed	Determination Of The Nucleotide Sequence Of Recombinant Plasmid Pbx6 Containing A Xylanase Gene From Fibrobacter Succinogenes S 85
title_sort	determination of the nucleotide sequence of recombinant plasmid pbx6 containing a xylanase gene from fibrobacter succinogenes s 85
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Environmental studies
publishDate	2001
url	http://psasir.upm.edu.my/id/eprint/11506/1/FSAS_2001_39.pdf
_version_	1804888639921979392

Determination Of The Nucleotide Sequence Of Recombinant Plasmid Pbx6 Containing A Xylanase Gene From Fibrobacter Succinogenes S 85

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