Development And Isolation Of Dna Microsatellite Markers For The Characterisation And Identification Of Mystus Nemurus (C & V)

Mystus nemurus or better known as 'ikan Baung' is an indigenous species In Malaysia. The popularity of this freshwater fish among the locals had made it an economically important aquaculture candidate. Of the so many DNA marker systems available, microsatellites are particularly useful...

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Main Author:	Chan, Soon Choy
Format:	Thesis
Language:	English English
Published:	2003
Subjects:	Microsatellites (Genetics) Bagre
Online Access:	http://psasir.upm.edu.my/id/eprint/11657/1/FSAS_2003_36.pdf
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id	my-upm-ir.11657
record_format	uketd_dc
spelling	my-upm-ir.116572024-06-24T07:00:24Z Development And Isolation Of Dna Microsatellite Markers For The Characterisation And Identification Of Mystus Nemurus (C & V) 2003-10 Chan, Soon Choy Mystus nemurus or better known as 'ikan Baung' is an indigenous species In Malaysia. The popularity of this freshwater fish among the locals had made it an economically important aquaculture candidate. Of the so many DNA marker systems available, microsatellites are particularly useful for population studies and have been proven to be the most efficient due to their codominant modes of inheritance. Two methods were used to isolate microsatellites in M. nemurus, namely Random Amplified Hybridisation Microsatellites (RAHMs) and Random Amplified Microsatellites (RAMs). A total of 88 micro satellite sequences and 18 cryptic simple regions were produced. The majority (90%) of these microsatellites were detected by the RAMs method while the RAHMs method required further optimisation and modification. The isolation of microsatellites resulted in 33 primer pairs being designed. Microsatellites (Genetics) Bagre 2003-10 Thesis http://psasir.upm.edu.my/id/eprint/11657/ http://psasir.upm.edu.my/id/eprint/11657/1/FSAS_2003_36.pdf text en public masters Universiti Putra Malaysia Microsatellites (Genetics) Bagre Faculty of Environmental studies Tan, Soon Guan English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Tan, Soon Guan
topic	Microsatellites (Genetics) Bagre
spellingShingle	Microsatellites (Genetics) Bagre Chan, Soon Choy Development And Isolation Of Dna Microsatellite Markers For The Characterisation And Identification Of Mystus Nemurus (C & V)
description	Mystus nemurus or better known as 'ikan Baung' is an indigenous species In Malaysia. The popularity of this freshwater fish among the locals had made it an economically important aquaculture candidate. Of the so many DNA marker systems available, microsatellites are particularly useful for population studies and have been proven to be the most efficient due to their codominant modes of inheritance. Two methods were used to isolate microsatellites in M. nemurus, namely Random Amplified Hybridisation Microsatellites (RAHMs) and Random Amplified Microsatellites (RAMs). A total of 88 micro satellite sequences and 18 cryptic simple regions were produced. The majority (90%) of these microsatellites were detected by the RAMs method while the RAHMs method required further optimisation and modification. The isolation of microsatellites resulted in 33 primer pairs being designed.
format	Thesis
qualification_level	Master's degree
author	Chan, Soon Choy
author_facet	Chan, Soon Choy
author_sort	Chan, Soon Choy
title	Development And Isolation Of Dna Microsatellite Markers For The Characterisation And Identification Of Mystus Nemurus (C & V)
title_short	Development And Isolation Of Dna Microsatellite Markers For The Characterisation And Identification Of Mystus Nemurus (C & V)
title_full	Development And Isolation Of Dna Microsatellite Markers For The Characterisation And Identification Of Mystus Nemurus (C & V)
title_fullStr	Development And Isolation Of Dna Microsatellite Markers For The Characterisation And Identification Of Mystus Nemurus (C & V)
title_full_unstemmed	Development And Isolation Of Dna Microsatellite Markers For The Characterisation And Identification Of Mystus Nemurus (C & V)
title_sort	development and isolation of dna microsatellite markers for the characterisation and identification of mystus nemurus (c & v)
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Environmental studies
publishDate	2003
url	http://psasir.upm.edu.my/id/eprint/11657/1/FSAS_2003_36.pdf
_version_	1804888645200510976

Development And Isolation Of Dna Microsatellite Markers For The Characterisation And Identification Of Mystus Nemurus (C & V)

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