The Effects of Newcastle Disease Virus (NDV) On Breast Cancer Cell Lines

Three Newcastle disease virus (NDV) isolates namely; F, Ijuk and 0l/C were tested for their anticancer properties against two breast cancer cell lines, MCF-7 and MDA-MB-231. Each virus strain was propagated in 10-day old embryonated eggs. Purification was carried out by density gradient centrifugati...

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Main Author:	Mohamad Wali, Haryati Shila
Format:	Thesis
Language:	English English
Published:	2003
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Online Access:	http://psasir.upm.edu.my/id/eprint/11679/1/FPV_2003_8_A.pdf
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spelling	my-upm-ir.116792013-05-27T07:49:40Z The Effects of Newcastle Disease Virus (NDV) On Breast Cancer Cell Lines 2003-08 Mohamad Wali, Haryati Shila Three Newcastle disease virus (NDV) isolates namely; F, Ijuk and 0l/C were tested for their anticancer properties against two breast cancer cell lines, MCF-7 and MDA-MB-231. Each virus strain was propagated in 10-day old embryonated eggs. Purification was carried out by density gradient centrifugation using sucrose. The titer of each virus strain was determined by hemagglutination (HA) test. Screening of NDV strains for anticancer properties on breast cancer cells was performed by a colorimetric cytotoxic assay using tetrazolium salt (MTT). F strain displayed cytotoxic activity on both breast cancer cells with an IC50 value of 8 HAU for MDA-MB-231 cells and 2048 HAU for MCF-7 cells. Meanwhile Ijuk showed cytotoxic activity against MDA-MB-231 cells only with an IC50 value of 8.6 HAD. Strain 01/C did not exhibit any cytotoxic activity towards both breast cancer cells. Inactivation of the virus at 100°C for 30 minutes destroyed its ability to kill the breast cancer cells. Positive control experiment involved treatment of the cells with tamoxifen, an estrogenic antagonist agent. Negative control experiment was carried out by infecting the virus onto normal mouse fibroblasts (3T3 cell). No cytotoxic activity was observed on 3T3 cells following infection at low virus titer. However, infection at higher virus titer resulted in 50% inhibition of cell growth. Infection of the virus displayed clear evidence of apoptosis which was detected as a ladder-like pattern on agarose gel electrophoresis. This was further confirmed by TEM which provided ultrastructural changes of the infected cells. The role of sialic acid receptor was also studied based on neuraminidase (NA) and sialyllactose (SLL) treatment. Treatment of the cells with NA did not destroy the ability of the virus to cause apoptosis. Meanwhile a reduction in the ability of the virus to cause apoptosis was observed in the treatment of SLL. However there was no significant difference between the SLL-treated virus (27.05%) and untreated virus (30.87%). Based on the results obtained, this study showed that NDV strains, F and ljuk have the potential to be developed as an anticancer agent. Mechanisms by which the virus infects and kills the cells need further studies. The role of sialic acid receptors in NDV-induced oncolytic effects requires further studies. 2003-08 Thesis http://psasir.upm.edu.my/id/eprint/11679/ http://psasir.upm.edu.my/id/eprint/11679/1/FPV_2003_8_A.pdf application/pdf en public masters Universiti Putra Malaysia Faculty of Veterinary Medicine English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic
spellingShingle	Mohamad Wali, Haryati Shila The Effects of Newcastle Disease Virus (NDV) On Breast Cancer Cell Lines
description	Three Newcastle disease virus (NDV) isolates namely; F, Ijuk and 0l/C were tested for their anticancer properties against two breast cancer cell lines, MCF-7 and MDA-MB-231. Each virus strain was propagated in 10-day old embryonated eggs. Purification was carried out by density gradient centrifugation using sucrose. The titer of each virus strain was determined by hemagglutination (HA) test. Screening of NDV strains for anticancer properties on breast cancer cells was performed by a colorimetric cytotoxic assay using tetrazolium salt (MTT). F strain displayed cytotoxic activity on both breast cancer cells with an IC50 value of 8 HAU for MDA-MB-231 cells and 2048 HAU for MCF-7 cells. Meanwhile Ijuk showed cytotoxic activity against MDA-MB-231 cells only with an IC50 value of 8.6 HAD. Strain 01/C did not exhibit any cytotoxic activity towards both breast cancer cells. Inactivation of the virus at 100°C for 30 minutes destroyed its ability to kill the breast cancer cells. Positive control experiment involved treatment of the cells with tamoxifen, an estrogenic antagonist agent. Negative control experiment was carried out by infecting the virus onto normal mouse fibroblasts (3T3 cell). No cytotoxic activity was observed on 3T3 cells following infection at low virus titer. However, infection at higher virus titer resulted in 50% inhibition of cell growth. Infection of the virus displayed clear evidence of apoptosis which was detected as a ladder-like pattern on agarose gel electrophoresis. This was further confirmed by TEM which provided ultrastructural changes of the infected cells. The role of sialic acid receptor was also studied based on neuraminidase (NA) and sialyllactose (SLL) treatment. Treatment of the cells with NA did not destroy the ability of the virus to cause apoptosis. Meanwhile a reduction in the ability of the virus to cause apoptosis was observed in the treatment of SLL. However there was no significant difference between the SLL-treated virus (27.05%) and untreated virus (30.87%). Based on the results obtained, this study showed that NDV strains, F and ljuk have the potential to be developed as an anticancer agent. Mechanisms by which the virus infects and kills the cells need further studies. The role of sialic acid receptors in NDV-induced oncolytic effects requires further studies.
format	Thesis
qualification_level	Master's degree
author	Mohamad Wali, Haryati Shila
author_facet	Mohamad Wali, Haryati Shila
author_sort	Mohamad Wali, Haryati Shila
title	The Effects of Newcastle Disease Virus (NDV) On Breast Cancer Cell Lines
title_short	The Effects of Newcastle Disease Virus (NDV) On Breast Cancer Cell Lines
title_full	The Effects of Newcastle Disease Virus (NDV) On Breast Cancer Cell Lines
title_fullStr	The Effects of Newcastle Disease Virus (NDV) On Breast Cancer Cell Lines
title_full_unstemmed	The Effects of Newcastle Disease Virus (NDV) On Breast Cancer Cell Lines
title_sort	effects of newcastle disease virus (ndv) on breast cancer cell lines
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Veterinary Medicine
publishDate	2003
url	http://psasir.upm.edu.my/id/eprint/11679/1/FPV_2003_8_A.pdf
_version_	1747811250453086208

The Effects of Newcastle Disease Virus (NDV) On Breast Cancer Cell Lines

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