Detection and Identification of Jaagsiekte Retrovirus Associated With Sheep Pulmonary Adenomatosis

Sheep pulmonary adenomatosis (SPA) or jaagsiekte is a neoplastic disease of sheep, caused by jaagsiekte retrovirus (JSRV) infection in the lungs. In Malaysia, the disease was first observed in 1993, involving mainly the purebred imported sheep and their crossbreeds, and a long time after the first i...

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Main Author:	Ze, Zhu
Format:	Thesis
Language:	English English
Published:	2002
Subjects:
Online Access:	http://psasir.upm.edu.my/id/eprint/11732/1/FPV_2002_5.pdf
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id	my-upm-ir.11732
record_format	uketd_dc
spelling	my-upm-ir.117322024-06-25T07:29:16Z Detection and Identification of Jaagsiekte Retrovirus Associated With Sheep Pulmonary Adenomatosis 2002-01 Ze, Zhu Sheep pulmonary adenomatosis (SPA) or jaagsiekte is a neoplastic disease of sheep, caused by jaagsiekte retrovirus (JSRV) infection in the lungs. In Malaysia, the disease was first observed in 1993, involving mainly the purebred imported sheep and their crossbreeds, and a long time after the first importation of sheep from Australia into Malaysia in 1987. The JSRV was found to transform the alveolar type II cells in the lungs of sheep to become neoplastic cuboidal cells arranged in acinar or papillary patterns. The neoplastic cells were found to cover the entire alveolar wall with numerous macrophages filling the affected alveoli particularly the alveoli surrounding the neoplastic area. The affected lung tissue and lung fluid were successfully processed to partially purify the materials before the JSRV was successfully detected under the electron microscopy from several local SPA-affected sheep, which showed clinical signs typical of sheep pulmonary adenomatosis. Following the detection of JSRV under the electron microscopy, the virus was later confirmed as the JSRV by using the reverse-transcription polymerase chain reaction (RT-PCR) technique at the viral nucleic acid level. The study. therefore, confirmed that JSRV is present in Malaysia and thus, should be considered in the diagnosis of sheep diseases and in controlling the spread of contagious diseases such as sheep pulmonary adenomatosis. Following the confirmation of the jaagsiekte retrovirus in Malaysia, a RTPCR diagnostic method was developed based on the U3 gene of the retrovirus. The method was able to identify the presence of retrovirus in the peripheral blood of sheep with and without clinical signs of sheep pulmonary adenomatosis. Thus, the technique can be used to identifY subclinically infected animals for culling. 2002-01 Thesis http://psasir.upm.edu.my/id/eprint/11732/ http://psasir.upm.edu.my/id/eprint/11732/1/FPV_2002_5.pdf text en public doctoral Universiti Putra Malaysia Faculty of Veterinary Medicine Saad, Mohd Zamri English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Saad, Mohd Zamri
topic
spellingShingle	Ze, Zhu Detection and Identification of Jaagsiekte Retrovirus Associated With Sheep Pulmonary Adenomatosis
description	Sheep pulmonary adenomatosis (SPA) or jaagsiekte is a neoplastic disease of sheep, caused by jaagsiekte retrovirus (JSRV) infection in the lungs. In Malaysia, the disease was first observed in 1993, involving mainly the purebred imported sheep and their crossbreeds, and a long time after the first importation of sheep from Australia into Malaysia in 1987. The JSRV was found to transform the alveolar type II cells in the lungs of sheep to become neoplastic cuboidal cells arranged in acinar or papillary patterns. The neoplastic cells were found to cover the entire alveolar wall with numerous macrophages filling the affected alveoli particularly the alveoli surrounding the neoplastic area. The affected lung tissue and lung fluid were successfully processed to partially purify the materials before the JSRV was successfully detected under the electron microscopy from several local SPA-affected sheep, which showed clinical signs typical of sheep pulmonary adenomatosis. Following the detection of JSRV under the electron microscopy, the virus was later confirmed as the JSRV by using the reverse-transcription polymerase chain reaction (RT-PCR) technique at the viral nucleic acid level. The study. therefore, confirmed that JSRV is present in Malaysia and thus, should be considered in the diagnosis of sheep diseases and in controlling the spread of contagious diseases such as sheep pulmonary adenomatosis. Following the confirmation of the jaagsiekte retrovirus in Malaysia, a RTPCR diagnostic method was developed based on the U3 gene of the retrovirus. The method was able to identify the presence of retrovirus in the peripheral blood of sheep with and without clinical signs of sheep pulmonary adenomatosis. Thus, the technique can be used to identifY subclinically infected animals for culling.
format	Thesis
qualification_level	Doctorate
author	Ze, Zhu
author_facet	Ze, Zhu
author_sort	Ze, Zhu
title	Detection and Identification of Jaagsiekte Retrovirus Associated With Sheep Pulmonary Adenomatosis
title_short	Detection and Identification of Jaagsiekte Retrovirus Associated With Sheep Pulmonary Adenomatosis
title_full	Detection and Identification of Jaagsiekte Retrovirus Associated With Sheep Pulmonary Adenomatosis
title_fullStr	Detection and Identification of Jaagsiekte Retrovirus Associated With Sheep Pulmonary Adenomatosis
title_full_unstemmed	Detection and Identification of Jaagsiekte Retrovirus Associated With Sheep Pulmonary Adenomatosis
title_sort	detection and identification of jaagsiekte retrovirus associated with sheep pulmonary adenomatosis
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Veterinary Medicine
publishDate	2002
url	http://psasir.upm.edu.my/id/eprint/11732/1/FPV_2002_5.pdf
_version_	1804888650990747648

Detection and Identification of Jaagsiekte Retrovirus Associated With Sheep Pulmonary Adenomatosis

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