In Vitro Production of Embryos from Abattoir-Derived Cattle Oocytes

Two studies involving some experiments were conducted to evaluate some factors affecting the in vitro production of cattle embryos from abattoir derived cattle oocytes. In the first study, more oocytes per ovary were recovered by slicing with a surgical blade (29.3 oocytes) than by aspiration with a...

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Main Author:	Sianturi, Riasari Gail
Format:	Thesis
Language:	English English
Published:	2001
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Online Access:	http://psasir.upm.edu.my/id/eprint/11741/1/FPV_2001_19_A.pdf
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id	my-upm-ir.11741
record_format	uketd_dc
spelling	my-upm-ir.117412011-09-07T00:53:48Z In Vitro Production of Embryos from Abattoir-Derived Cattle Oocytes 2001-07 Sianturi, Riasari Gail Two studies involving some experiments were conducted to evaluate some factors affecting the in vitro production of cattle embryos from abattoir derived cattle oocytes. In the first study, more oocytes per ovary were recovered by slicing with a surgical blade (29.3 oocytes) than by aspiration with a disposable syringe and needle (12.0 oocytes). Cumulus expansion rate and maturation rate were better in oocytes surrounded by cumulus cells than in denuded oocytes and fibrinated oocytes. To determine the influence of adding serum and hormones, cumulus oocyte complexes (COCs) were matured in four different maturation media and incubated for 22 h at 39°C with 5% CO2 in humidified air. The addition of hormones to the maturation medium enhanced cumulus expansion rate and maturation rate. In the absence of hormones, 20% serum level rendered better cumulus expansion than with 10% serum but had no effect on the maturation rate. In the second study, factors affecting the IVF and the developmental competence of embryos were studied. In vitro matured oocytes were inseminated with swim-up separated sperm in IVF-TALP medium. At 18 or 44 h post insemination, the presumptive embryos were freed of cumulus and transferred into two culture media (lVC): modilled synthetic oviductal fluid (mSOF) as cell-free culture system and M199 with bovine oviductal epithelial cell (BOEC) as co-culture system. At 6 hour after inseminaton, male pronucleus formation was first observed. There were no signmcant differences on the effect of serum level (10% or 20%) and hormones supplementation in the maturation medium on the cleavage rate and developmental competence of embryos. Cleavage and blastocyst rates were 71.2% and 6.2% for cumulus-intact oocytes whereas the rates were 47.2% and 1.9% for cumulus-free oocytes. Although the cleavage rate was not different, better morula and blastocyst rates were obtained from co-culture system. The results indicate that hormones enhance cumulus cells expansion and maturation rates, cumulus cells facilitate fertilization while co-culture with BOEe rendered better developmental capacity of embryos. However, the failure of morula to develop to blastocysts in vitro needs further study. 2001-07 Thesis http://psasir.upm.edu.my/id/eprint/11741/ http://psasir.upm.edu.my/id/eprint/11741/1/FPV_2001_19_A.pdf application/pdf en public masters Universiti Putra Malaysia Faculty of Veterinary Medicine English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic
spellingShingle	Sianturi, Riasari Gail In Vitro Production of Embryos from Abattoir-Derived Cattle Oocytes
description	Two studies involving some experiments were conducted to evaluate some factors affecting the in vitro production of cattle embryos from abattoir derived cattle oocytes. In the first study, more oocytes per ovary were recovered by slicing with a surgical blade (29.3 oocytes) than by aspiration with a disposable syringe and needle (12.0 oocytes). Cumulus expansion rate and maturation rate were better in oocytes surrounded by cumulus cells than in denuded oocytes and fibrinated oocytes. To determine the influence of adding serum and hormones, cumulus oocyte complexes (COCs) were matured in four different maturation media and incubated for 22 h at 39°C with 5% CO2 in humidified air. The addition of hormones to the maturation medium enhanced cumulus expansion rate and maturation rate. In the absence of hormones, 20% serum level rendered better cumulus expansion than with 10% serum but had no effect on the maturation rate. In the second study, factors affecting the IVF and the developmental competence of embryos were studied. In vitro matured oocytes were inseminated with swim-up separated sperm in IVF-TALP medium. At 18 or 44 h post insemination, the presumptive embryos were freed of cumulus and transferred into two culture media (lVC): modilled synthetic oviductal fluid (mSOF) as cell-free culture system and M199 with bovine oviductal epithelial cell (BOEC) as co-culture system. At 6 hour after inseminaton, male pronucleus formation was first observed. There were no signmcant differences on the effect of serum level (10% or 20%) and hormones supplementation in the maturation medium on the cleavage rate and developmental competence of embryos. Cleavage and blastocyst rates were 71.2% and 6.2% for cumulus-intact oocytes whereas the rates were 47.2% and 1.9% for cumulus-free oocytes. Although the cleavage rate was not different, better morula and blastocyst rates were obtained from co-culture system. The results indicate that hormones enhance cumulus cells expansion and maturation rates, cumulus cells facilitate fertilization while co-culture with BOEe rendered better developmental capacity of embryos. However, the failure of morula to develop to blastocysts in vitro needs further study.
format	Thesis
qualification_level	Master's degree
author	Sianturi, Riasari Gail
author_facet	Sianturi, Riasari Gail
author_sort	Sianturi, Riasari Gail
title	In Vitro Production of Embryos from Abattoir-Derived Cattle Oocytes
title_short	In Vitro Production of Embryos from Abattoir-Derived Cattle Oocytes
title_full	In Vitro Production of Embryos from Abattoir-Derived Cattle Oocytes
title_fullStr	In Vitro Production of Embryos from Abattoir-Derived Cattle Oocytes
title_full_unstemmed	In Vitro Production of Embryos from Abattoir-Derived Cattle Oocytes
title_sort	in vitro production of embryos from abattoir-derived cattle oocytes
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Veterinary Medicine
publishDate	2001
url	http://psasir.upm.edu.my/id/eprint/11741/1/FPV_2001_19_A.pdf
_version_	1747811255841718272

In Vitro Production of Embryos from Abattoir-Derived Cattle Oocytes

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