Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan

Siamese fighting fish, Betta splendens Regan mortalities in Thailand are usually associated with mycobacteriosis. The present study was undertaken to isolate and characterise mycobacteria from Siamese fighting fish, examine their sensitivity to selected antibiotics and to test the efficacy of k...

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Main Author:	Pungkachonboon, Temdoung
Format:	Thesis
Language:	English English
Published:	1994
Subjects:	Siamese fighting fish - Diseases - Research Mycobacterial diseases in animals
Online Access:	http://psasir.upm.edu.my/id/eprint/11792/1/FPSS_1994_1_A.pdf
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id	my-upm-ir.11792
record_format	uketd_dc
spelling	my-upm-ir.117922011-09-09T01:26:23Z Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan 1994-02 Pungkachonboon, Temdoung Siamese fighting fish, Betta splendens Regan mortalities in Thailand are usually associated with mycobacteriosis. The present study was undertaken to isolate and characterise mycobacteria from Siamese fighting fish, examine their sensitivity to selected antibiotics and to test the efficacy of kanamycin sulphate as a chemotherapeutic agent against mycobacteria. Histopathological studies on the Siamese fighting fish, experimentally and naturally infected with mycobacteria, were also conducted.Ten acid-fast, rapidly growing, photochromogenic strains of mycobacteria were isolated from apparently healthy fish and fish showing gross lesions of mycobacteriosis. Prevalence of mycobacteria in farm reared Siamese fighting fish ranged from 0-8%. Primary isolation of mycobacteria were successful on Ogawa egg medium and Lowenstein Jensen medium. The isolates grew within 5-7 days at 28°C on Ogawa egg medium and showed a temperature preference of 15- 17 °C. No growth was observed at 42°C. Optimum incubation temperature was 30°C. They were considered as mesophyllic forms. The organism produced mycolic acid which melted between 50.5-7 0°C. On the basis of their biochemical and physiological properties and the percentage guanine plus cytosine, the pathogen was confirmed as Mycobacterium sp. and was analogous to " M . piscicida " with similarity of 94% , but differed from the reference strains M. chelonae sub sp. chelonae , M . fortuitum , M . marinum , M . parafortuitum and M . vaccae by many characteristics. An antibiotic sensitivity test was conducted using seventeen antimicrobial agents against ten mycobacteria strains isolated from Siamese fighting fish. Kanamycin at 30 ug was the most effective antibiotic against mycobacteria. MIC and MBC values of kanamycin sulphate were 6 .25-12.50 and 12.5-25.0 ppm, respectively. However, treatment of mycobacteriosis with kanamycin, in vivo , was unsuccessful. Siamese fighting fish - Diseases - Research Mycobacterial diseases in animals 1994-02 Thesis http://psasir.upm.edu.my/id/eprint/11792/ http://psasir.upm.edu.my/id/eprint/11792/1/FPSS_1994_1_A.pdf application/pdf en public phd doctoral Universiti Pertanian Malaysia Siamese fighting fish - Diseases - Research Mycobacterial diseases in animals Faculty of Fisheries English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Siamese fighting fish - Diseases - Research Mycobacterial diseases in animals
spellingShingle	Siamese fighting fish - Diseases - Research Mycobacterial diseases in animals Pungkachonboon, Temdoung Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan
description	Siamese fighting fish, Betta splendens Regan mortalities in Thailand are usually associated with mycobacteriosis. The present study was undertaken to isolate and characterise mycobacteria from Siamese fighting fish, examine their sensitivity to selected antibiotics and to test the efficacy of kanamycin sulphate as a chemotherapeutic agent against mycobacteria. Histopathological studies on the Siamese fighting fish, experimentally and naturally infected with mycobacteria, were also conducted.Ten acid-fast, rapidly growing, photochromogenic strains of mycobacteria were isolated from apparently healthy fish and fish showing gross lesions of mycobacteriosis. Prevalence of mycobacteria in farm reared Siamese fighting fish ranged from 0-8%. Primary isolation of mycobacteria were successful on Ogawa egg medium and Lowenstein Jensen medium. The isolates grew within 5-7 days at 28°C on Ogawa egg medium and showed a temperature preference of 15- 17 °C. No growth was observed at 42°C. Optimum incubation temperature was 30°C. They were considered as mesophyllic forms. The organism produced mycolic acid which melted between 50.5-7 0°C. On the basis of their biochemical and physiological properties and the percentage guanine plus cytosine, the pathogen was confirmed as Mycobacterium sp. and was analogous to " M . piscicida " with similarity of 94% , but differed from the reference strains M. chelonae sub sp. chelonae , M . fortuitum , M . marinum , M . parafortuitum and M . vaccae by many characteristics. An antibiotic sensitivity test was conducted using seventeen antimicrobial agents against ten mycobacteria strains isolated from Siamese fighting fish. Kanamycin at 30 ug was the most effective antibiotic against mycobacteria. MIC and MBC values of kanamycin sulphate were 6 .25-12.50 and 12.5-25.0 ppm, respectively. However, treatment of mycobacteriosis with kanamycin, in vivo , was unsuccessful.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Pungkachonboon, Temdoung
author_facet	Pungkachonboon, Temdoung
author_sort	Pungkachonboon, Temdoung
title	Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan
title_short	Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan
title_full	Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan
title_fullStr	Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan
title_full_unstemmed	Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan
title_sort	studies on mycobacteriosis in siamese fighting fish betta splendens regan
granting_institution	Universiti Pertanian Malaysia
granting_department	Faculty of Fisheries
publishDate	1994
url	http://psasir.upm.edu.my/id/eprint/11792/1/FPSS_1994_1_A.pdf
_version_	1747811263895830528

Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan

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