Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan
Siamese fighting fish, Betta splendens Regan mortalities in Thailand are usually associated with mycobacteriosis. The present study was undertaken to isolate and characterise mycobacteria from Siamese fighting fish, examine their sensitivity to selected antibiotics and to test the efficacy of k...
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1994
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my-upm-ir.117922011-09-09T01:26:23Z Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan 1994-02 Pungkachonboon, Temdoung Siamese fighting fish, Betta splendens Regan mortalities in Thailand are usually associated with mycobacteriosis. The present study was undertaken to isolate and characterise mycobacteria from Siamese fighting fish, examine their sensitivity to selected antibiotics and to test the efficacy of kanamycin sulphate as a chemotherapeutic agent against mycobacteria. Histopathological studies on the Siamese fighting fish, experimentally and naturally infected with mycobacteria, were also conducted.Ten acid-fast, rapidly growing, photochromogenic strains of mycobacteria were isolated from apparently healthy fish and fish showing gross lesions of mycobacteriosis. Prevalence of mycobacteria in farm reared Siamese fighting fish ranged from 0-8%. Primary isolation of mycobacteria were successful on Ogawa egg medium and Lowenstein Jensen medium. The isolates grew within 5-7 days at 28°C on Ogawa egg medium and showed a temperature preference of 15- 17 °C. No growth was observed at 42°C. Optimum incubation temperature was 30°C. They were considered as mesophyllic forms. The organism produced mycolic acid which melted between 50.5-7 0°C. On the basis of their biochemical and physiological properties and the percentage guanine plus cytosine, the pathogen was confirmed as Mycobacterium sp. and was analogous to " M . piscicida " with similarity of 94% , but differed from the reference strains M. chelonae sub sp. chelonae , M . fortuitum , M . marinum , M . parafortuitum and M . vaccae by many characteristics. An antibiotic sensitivity test was conducted using seventeen antimicrobial agents against ten mycobacteria strains isolated from Siamese fighting fish. Kanamycin at 30 ug was the most effective antibiotic against mycobacteria. MIC and MBC values of kanamycin sulphate were 6 .25-12.50 and 12.5-25.0 ppm, respectively. However, treatment of mycobacteriosis with kanamycin, in vivo , was unsuccessful. Siamese fighting fish - Diseases - Research Mycobacterial diseases in animals 1994-02 Thesis http://psasir.upm.edu.my/id/eprint/11792/ http://psasir.upm.edu.my/id/eprint/11792/1/FPSS_1994_1_A.pdf application/pdf en public phd doctoral Universiti Pertanian Malaysia Siamese fighting fish - Diseases - Research Mycobacterial diseases in animals Faculty of Fisheries English |
| institution |
Universiti Putra Malaysia |
| collection |
PSAS Institutional Repository |
| language |
English English |
| topic |
Siamese fighting fish - Diseases - Research Mycobacterial diseases in animals |
| spellingShingle |
Siamese fighting fish - Diseases - Research Mycobacterial diseases in animals Pungkachonboon, Temdoung Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan |
| description |
Siamese fighting fish, Betta splendens Regan
mortalities in Thailand are usually associated with
mycobacteriosis. The present study was undertaken to
isolate and characterise mycobacteria from Siamese fighting
fish, examine their sensitivity to selected antibiotics and
to test the efficacy of kanamycin sulphate as a
chemotherapeutic agent against mycobacteria.
Histopathological studies on the Siamese fighting fish,
experimentally and naturally infected with mycobacteria,
were also conducted.Ten acid-fast, rapidly growing, photochromogenic
strains of mycobacteria were isolated from apparently
healthy fish and fish showing gross lesions of
mycobacteriosis. Prevalence of mycobacteria in farm reared Siamese fighting fish ranged from 0-8%. Primary isolation
of mycobacteria were successful on Ogawa egg medium and
Lowenstein Jensen medium. The isolates grew within 5-7
days at 28°C on Ogawa egg medium and showed a temperature
preference of 15- 17 °C. No growth was observed at 42°C.
Optimum incubation temperature was 30°C. They were
considered as mesophyllic forms. The organism produced
mycolic acid which melted between 50.5-7 0°C. On the basis
of their biochemical and physiological properties and the
percentage guanine plus cytosine, the pathogen was
confirmed as Mycobacterium sp. and was analogous to " M .
piscicida " with similarity of 94% , but differed from the
reference strains M. chelonae sub sp. chelonae , M .
fortuitum , M . marinum , M . parafortuitum and M . vaccae by
many characteristics. An antibiotic sensitivity test was conducted using
seventeen antimicrobial agents against ten mycobacteria
strains isolated from Siamese fighting fish. Kanamycin at
30 ug was the most effective antibiotic against
mycobacteria. MIC and MBC values of kanamycin sulphate
were 6 .25-12.50 and 12.5-25.0 ppm, respectively. However,
treatment of mycobacteriosis with kanamycin, in vivo , was
unsuccessful. |
| format |
Thesis |
| qualification_name |
Doctor of Philosophy (PhD.) |
| qualification_level |
Doctorate |
| author |
Pungkachonboon, Temdoung |
| author_facet |
Pungkachonboon, Temdoung |
| author_sort |
Pungkachonboon, Temdoung |
| title |
Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan
|
| title_short |
Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan
|
| title_full |
Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan
|
| title_fullStr |
Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan
|
| title_full_unstemmed |
Studies On Mycobacteriosis In Siamese Fighting Fish Betta Splendens Regan
|
| title_sort |
studies on mycobacteriosis in siamese fighting fish betta splendens regan |
| granting_institution |
Universiti Pertanian Malaysia |
| granting_department |
Faculty of Fisheries |
| publishDate |
1994 |
| url |
http://psasir.upm.edu.my/id/eprint/11792/1/FPSS_1994_1_A.pdf |
| _version_ |
1747811263895830528 |