Effect Of Copper On The Non-Specific Immune Response Of Puntius Gonionotus Challenged With Aeromonas Hydrophila

This study is focused on toxicity of copper and its effects on the immune response of P. gonionotus. The median lethal concentration of copper (LC50) on P. gonionotus (56.9±4.5 g) at 12, 24, 48, 72 and 120 hours was estimated as 2.17, 0.91, 0.57, 0. 53, 0.42 mg/L respectively. Fish were intraper...

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Main Author:	Jayawardena, P.A.H.L.
Format:	Thesis
Language:	English English
Published:	1996
Subjects:	Universiti Pertanian Malaysia - Dissertations Fishes - Infections Aeromonas hydrophila
Online Access:	http://psasir.upm.edu.my/id/eprint/11820/1/FPSS_1996_3_A.pdf
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id	my-upm-ir.11820
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spelling	my-upm-ir.118202011-09-08T07:51:15Z Effect Of Copper On The Non-Specific Immune Response Of Puntius Gonionotus Challenged With Aeromonas Hydrophila 1996-12 Jayawardena, P.A.H.L. This study is focused on toxicity of copper and its effects on the immune response of P. gonionotus. The median lethal concentration of copper (LC50) on P. gonionotus (56.9±4.5 g) at 12, 24, 48, 72 and 120 hours was estimated as 2.17, 0.91, 0.57, 0. 53, 0.42 mg/L respectively. Fish were intraperitonealy injected with three different doses (4.5 x 105, 4.5 X 107 and 4.5 x 109 CFU/mL) of formalin killed A. hydrophila (0.1 mL) and the immune responses were monitored using haematological and serological assays over a period of 30 days post-injection. The assays used were total WBC counts, lysozyme, NBT, total protein, total immunoglobulin and haematocrit levels. The immune responses with the highest magnitude were observed among fish injected with 4.5 x 109 and the lowest among 4.5 x 105 CFU/mL. The responses were observed to be both time and dose dependent.To find the effect of copper on the immune response, fish were exposed to different sublethal concentrations of copper, determined at 10%, 20%, and 30% of 96 hour LC50 for 71 days. After 56 days of initial exposure, fish were challenged with 0.1 mL of 4.5 x 105 CFU/mL formalin killed A. hydrophila. The immune responses were monitored using the same immunological assays mentioned above withdrawing non-lethal blood samples at 14, 28, 56, 61, 66 and 71 days of exposure to copper. Though a depletion of protein and immunoglobulin titres in plasma were observed at the initial exposure to copper, it did not influence the increased titres demonstrated against the bacterial challenge in any of the treatments. In contrast, the WBC counts increased during the initial exposure to copper. WBC counts, lysozyme and NBT assays showed suppressed immune responses after challenging with bacteria compared to the control group. But the responses observed at the lowest concentration (0.05 mg/L) of exposure to copper, were similar to that of the control in most of the cases. This suggests the absence of immunosuppressive effects at 0.05 mg/L of copper. Universiti Pertanian Malaysia - Dissertations Fishes - Infections Aeromonas hydrophila 1996-12 Thesis http://psasir.upm.edu.my/id/eprint/11820/ http://psasir.upm.edu.my/id/eprint/11820/1/FPSS_1996_3_A.pdf application/pdf en public masters Universiti Pertanian Malaysia Universiti Pertanian Malaysia - Dissertations Fishes - Infections Aeromonas hydrophila Faculty of Fisheries English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Universiti Pertanian Malaysia - Dissertations Fishes - Infections Aeromonas hydrophila
spellingShingle	Universiti Pertanian Malaysia - Dissertations Fishes - Infections Aeromonas hydrophila Jayawardena, P.A.H.L. Effect Of Copper On The Non-Specific Immune Response Of Puntius Gonionotus Challenged With Aeromonas Hydrophila
description	This study is focused on toxicity of copper and its effects on the immune response of P. gonionotus. The median lethal concentration of copper (LC50) on P. gonionotus (56.9±4.5 g) at 12, 24, 48, 72 and 120 hours was estimated as 2.17, 0.91, 0.57, 0. 53, 0.42 mg/L respectively. Fish were intraperitonealy injected with three different doses (4.5 x 105, 4.5 X 107 and 4.5 x 109 CFU/mL) of formalin killed A. hydrophila (0.1 mL) and the immune responses were monitored using haematological and serological assays over a period of 30 days post-injection. The assays used were total WBC counts, lysozyme, NBT, total protein, total immunoglobulin and haematocrit levels. The immune responses with the highest magnitude were observed among fish injected with 4.5 x 109 and the lowest among 4.5 x 105 CFU/mL. The responses were observed to be both time and dose dependent.To find the effect of copper on the immune response, fish were exposed to different sublethal concentrations of copper, determined at 10%, 20%, and 30% of 96 hour LC50 for 71 days. After 56 days of initial exposure, fish were challenged with 0.1 mL of 4.5 x 105 CFU/mL formalin killed A. hydrophila. The immune responses were monitored using the same immunological assays mentioned above withdrawing non-lethal blood samples at 14, 28, 56, 61, 66 and 71 days of exposure to copper. Though a depletion of protein and immunoglobulin titres in plasma were observed at the initial exposure to copper, it did not influence the increased titres demonstrated against the bacterial challenge in any of the treatments. In contrast, the WBC counts increased during the initial exposure to copper. WBC counts, lysozyme and NBT assays showed suppressed immune responses after challenging with bacteria compared to the control group. But the responses observed at the lowest concentration (0.05 mg/L) of exposure to copper, were similar to that of the control in most of the cases. This suggests the absence of immunosuppressive effects at 0.05 mg/L of copper.
format	Thesis
qualification_level	Master's degree
author	Jayawardena, P.A.H.L.
author_facet	Jayawardena, P.A.H.L.
author_sort	Jayawardena, P.A.H.L.
title	Effect Of Copper On The Non-Specific Immune Response Of Puntius Gonionotus Challenged With Aeromonas Hydrophila
title_short	Effect Of Copper On The Non-Specific Immune Response Of Puntius Gonionotus Challenged With Aeromonas Hydrophila
title_full	Effect Of Copper On The Non-Specific Immune Response Of Puntius Gonionotus Challenged With Aeromonas Hydrophila
title_fullStr	Effect Of Copper On The Non-Specific Immune Response Of Puntius Gonionotus Challenged With Aeromonas Hydrophila
title_full_unstemmed	Effect Of Copper On The Non-Specific Immune Response Of Puntius Gonionotus Challenged With Aeromonas Hydrophila
title_sort	effect of copper on the non-specific immune response of puntius gonionotus challenged with aeromonas hydrophila
granting_institution	Universiti Pertanian Malaysia
granting_department	Faculty of Fisheries
publishDate	1996
url	http://psasir.upm.edu.my/id/eprint/11820/1/FPSS_1996_3_A.pdf
_version_	1747811269279219712

Effect Of Copper On The Non-Specific Immune Response Of Puntius Gonionotus Challenged With Aeromonas Hydrophila

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