Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases
Enzymatic transesterification of palm olein (POo) and cod liver oil (CLO) in a solvent free system was studied using immobilized enzymes, with the aim of enriching POo with essential fatty acids/polyunsaturated fatty acids (PUF A) from CLO. The effects of enzyme loading, different ratio of POo an...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2001
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/11855/1/FSMB_2001_38.pdf |
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| Summary: | Enzymatic transesterification of palm olein (POo) and cod liver oil (CLO)
in a solvent free system was studied using immobilized enzymes, with the aim of
enriching POo with essential fatty acids/polyunsaturated fatty acids (PUF A) from
CLO. The effects of enzyme loading, different ratio of POo and CLO blends, and
the enzyme's regiospecificity on the triglyceride ( TG) profile and fatty acid
composition were evaluated. Reverse-phase high performance liquid
chromatography (HPLC) and alkaline titration evaluated the catalytic
performance of the lipase in terms of degree of transesterification and hydrolysis,
respectively. The fatty acid (FA) composition of glycerides was studied using
gas chromatography (GC). The 1,3-specific and non-specific lipases used were
Rhizomucor miehei lipase ( Lipozyme IM60) and Pseudomonas cepacia lipase,
respectively. Results obtained show that the degree of transesterification and
hydrolysis increased with an increase of R. miehei lipase concentration.The fatty acid composition based on relative concentrations of the mixtures before and
after reaction showed no significant changes. |
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