Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases

Enzymatic transesterification of palm olein (POo) and cod liver oil (CLO) in a solvent free system was studied using immobilized enzymes, with the aim of enriching POo with essential fatty acids/polyunsaturated fatty acids (PUF A) from CLO. The effects of enzyme loading, different ratio of POo an...

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Main Author:	Chew , Pauline Xui Sim
Format:	Thesis
Language:	English English
Published:	2001
Subjects:	Transesterification. Immobilized enzymes. Hydrolysis.
Online Access:	http://psasir.upm.edu.my/id/eprint/11855/1/FSMB_2001_38_A.pdf
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id	my-upm-ir.11855
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spelling	my-upm-ir.118552012-07-04T04:51:50Z Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases 2001-07 Chew , Pauline Xui Sim Enzymatic transesterification of palm olein (POo) and cod liver oil (CLO) in a solvent free system was studied using immobilized enzymes, with the aim of enriching POo with essential fatty acids/polyunsaturated fatty acids (PUF A) from CLO. The effects of enzyme loading, different ratio of POo and CLO blends, and the enzyme's regiospecificity on the triglyceride ( TG) profile and fatty acid composition were evaluated. Reverse-phase high performance liquid chromatography (HPLC) and alkaline titration evaluated the catalytic performance of the lipase in terms of degree of transesterification and hydrolysis, respectively. The fatty acid (FA) composition of glycerides was studied using gas chromatography (GC). The 1,3-specific and non-specific lipases used were Rhizomucor miehei lipase ( Lipozyme IM60) and Pseudomonas cepacia lipase, respectively. Results obtained show that the degree of transesterification and hydrolysis increased with an increase of R. miehei lipase concentration.The fatty acid composition based on relative concentrations of the mixtures before and after reaction showed no significant changes. Transesterification. Immobilized enzymes. Hydrolysis. 2001-07 Thesis http://psasir.upm.edu.my/id/eprint/11855/ http://psasir.upm.edu.my/id/eprint/11855/1/FSMB_2001_38_A.pdf application/pdf en public masters Universiti Putra Malaysia Transesterification. Immobilized enzymes. Hydrolysis. Faculty of Food Science and Biotechnology English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Transesterification. Immobilized enzymes. Hydrolysis.
spellingShingle	Transesterification. Immobilized enzymes. Hydrolysis. Chew , Pauline Xui Sim Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases
description	Enzymatic transesterification of palm olein (POo) and cod liver oil (CLO) in a solvent free system was studied using immobilized enzymes, with the aim of enriching POo with essential fatty acids/polyunsaturated fatty acids (PUF A) from CLO. The effects of enzyme loading, different ratio of POo and CLO blends, and the enzyme's regiospecificity on the triglyceride ( TG) profile and fatty acid composition were evaluated. Reverse-phase high performance liquid chromatography (HPLC) and alkaline titration evaluated the catalytic performance of the lipase in terms of degree of transesterification and hydrolysis, respectively. The fatty acid (FA) composition of glycerides was studied using gas chromatography (GC). The 1,3-specific and non-specific lipases used were Rhizomucor miehei lipase ( Lipozyme IM60) and Pseudomonas cepacia lipase, respectively. Results obtained show that the degree of transesterification and hydrolysis increased with an increase of R. miehei lipase concentration.The fatty acid composition based on relative concentrations of the mixtures before and after reaction showed no significant changes.
format	Thesis
qualification_level	Master's degree
author	Chew , Pauline Xui Sim
author_facet	Chew , Pauline Xui Sim
author_sort	Chew , Pauline Xui Sim
title	Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases
title_short	Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases
title_full	Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases
title_fullStr	Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases
title_full_unstemmed	Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases
title_sort	enzymatic transesterification of palm olein and cod liver oil by immobilized rhizomucor miehei and pseudomonas cepacia lipases
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Food Science and Biotechnology
publishDate	2001
url	http://psasir.upm.edu.my/id/eprint/11855/1/FSMB_2001_38_A.pdf
_version_	1747811273703161856

Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases

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