Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases

Enzymatic transesterification of palm olein (POo) and cod liver oil (CLO) in a solvent free system was studied using immobilized enzymes, with the aim of enriching POo with essential fatty acids/polyunsaturated fatty acids (PUF A) from CLO. The effects of enzyme loading, different ratio of POo an...

全面介绍

Saved in:

书目详细资料
主要作者:	Chew, Pauline Xui Sim
格式:	Thesis
语言:	English English
出版:	2001
主题:	Transesterification. Immobilized enzymes. Hydrolysis.
在线阅读:	http://psasir.upm.edu.my/id/eprint/11855/1/FSMB_2001_38.pdf
标签:	添加标签没有标签, 成为第一个标记此记录!

id	my-upm-ir.11855
record_format	uketd_dc
spelling	my-upm-ir.118552024-06-26T09:01:39Z Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases 2001-07 Chew, Pauline Xui Sim Enzymatic transesterification of palm olein (POo) and cod liver oil (CLO) in a solvent free system was studied using immobilized enzymes, with the aim of enriching POo with essential fatty acids/polyunsaturated fatty acids (PUF A) from CLO. The effects of enzyme loading, different ratio of POo and CLO blends, and the enzyme's regiospecificity on the triglyceride ( TG) profile and fatty acid composition were evaluated. Reverse-phase high performance liquid chromatography (HPLC) and alkaline titration evaluated the catalytic performance of the lipase in terms of degree of transesterification and hydrolysis, respectively. The fatty acid (FA) composition of glycerides was studied using gas chromatography (GC). The 1,3-specific and non-specific lipases used were Rhizomucor miehei lipase ( Lipozyme IM60) and Pseudomonas cepacia lipase, respectively. Results obtained show that the degree of transesterification and hydrolysis increased with an increase of R. miehei lipase concentration.The fatty acid composition based on relative concentrations of the mixtures before and after reaction showed no significant changes. Transesterification. Immobilized enzymes. Hydrolysis. 2001-07 Thesis http://psasir.upm.edu.my/id/eprint/11855/ http://psasir.upm.edu.my/id/eprint/11855/1/FSMB_2001_38.pdf text en public masters Universiti Putra Malaysia Transesterification. Immobilized enzymes. Hydrolysis. Faculty of Food Science and Biotechnology Mohd. Ghazali, Hasanah English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Mohd. Ghazali, Hasanah
topic	Transesterification. Immobilized enzymes. Hydrolysis.
spellingShingle	Transesterification. Immobilized enzymes. Hydrolysis. Chew, Pauline Xui Sim Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases
description	Enzymatic transesterification of palm olein (POo) and cod liver oil (CLO) in a solvent free system was studied using immobilized enzymes, with the aim of enriching POo with essential fatty acids/polyunsaturated fatty acids (PUF A) from CLO. The effects of enzyme loading, different ratio of POo and CLO blends, and the enzyme's regiospecificity on the triglyceride ( TG) profile and fatty acid composition were evaluated. Reverse-phase high performance liquid chromatography (HPLC) and alkaline titration evaluated the catalytic performance of the lipase in terms of degree of transesterification and hydrolysis, respectively. The fatty acid (FA) composition of glycerides was studied using gas chromatography (GC). The 1,3-specific and non-specific lipases used were Rhizomucor miehei lipase ( Lipozyme IM60) and Pseudomonas cepacia lipase, respectively. Results obtained show that the degree of transesterification and hydrolysis increased with an increase of R. miehei lipase concentration.The fatty acid composition based on relative concentrations of the mixtures before and after reaction showed no significant changes.
format	Thesis
qualification_level	Master's degree
author	Chew, Pauline Xui Sim
author_facet	Chew, Pauline Xui Sim
author_sort	Chew, Pauline Xui Sim
title	Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases
title_short	Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases
title_full	Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases
title_fullStr	Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases
title_full_unstemmed	Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases
title_sort	enzymatic transesterification of palm olein and cod liver oil by immobilized rhizomucor miehei and pseudomonas cepacia lipases
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Food Science and Biotechnology
publishDate	2001
url	http://psasir.upm.edu.my/id/eprint/11855/1/FSMB_2001_38.pdf
_version_	1804888660075610112

Enzymatic Transesterification Of Palm Olein And Cod Liver Oil By Immobilized Rhizomucor Miehei And Pseudomonas Cepacia Lipases

相似书籍