Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate

The performance of direct fermentation of sago starch to ethanol by three strains of genetically modified Saccharomyces cerevisiae, YKU 107 (expressing a.-amylase), YKU 131 (expressing glucoamylase) and YKU 132 (expressing a.-amylase and glucoamylase), was investigated. In fermentation using 2 L...

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Main Author:	Ang, Dek Chang
Format:	Thesis
Language:	English English
Published:	2001
Subjects:	Saccharomyces cerevisiae. Transgenic organisms. Fermentation.
Online Access:	http://psasir.upm.edu.my/id/eprint/11856/1/FSMB_2001_39.pdf
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id	my-upm-ir.11856
record_format	uketd_dc
spelling	my-upm-ir.118562024-06-26T09:04:30Z Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate 2001-06 Ang, Dek Chang The performance of direct fermentation of sago starch to ethanol by three strains of genetically modified Saccharomyces cerevisiae, YKU 107 (expressing a.-amylase), YKU 131 (expressing glucoamylase) and YKU 132 (expressing a.-amylase and glucoamylase), was investigated. In fermentation using 2 L fermenter, we found that strains YKU 107, YKU 131 and YKU 132 was capable to hydrolyse 83.45%,67.45% and 71.9% of sago starch, respectively. However, only strain YKU 131 has the ability to produce significant amount of ethanol (2.16 gL-1) from sago starch. The superiority of strain YKU 131 as compared to strain YKU 107 and YKU 132 was found to be correlated with its ability to secrete glucoamylase. S. cerevisiae YKU 131 was selected for further studies. To investigate the performance of S. cerevlszae YKU 131 in ethanol production using various types of starch, initial starch concentration was fixed at 20 gL-1 Among different types of starch investigated, com starch was the most susceptible to the glucoamylase, followed by sago, cassava, potato and rice starch. Saccharomyces cerevisiae. Transgenic organisms. Fermentation. 2001-06 Thesis http://psasir.upm.edu.my/id/eprint/11856/ http://psasir.upm.edu.my/id/eprint/11856/1/FSMB_2001_39.pdf text en public masters Universiti Putra Malaysia Saccharomyces cerevisiae. Transgenic organisms. Fermentation. Faculty of Food Science and Biotechnology Abd. Aziz, Suraini English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Abd. Aziz, Suraini
topic	Saccharomyces cerevisiae. Transgenic organisms. Fermentation.
spellingShingle	Saccharomyces cerevisiae. Transgenic organisms. Fermentation. Ang, Dek Chang Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate
description	The performance of direct fermentation of sago starch to ethanol by three strains of genetically modified Saccharomyces cerevisiae, YKU 107 (expressing a.-amylase), YKU 131 (expressing glucoamylase) and YKU 132 (expressing a.-amylase and glucoamylase), was investigated. In fermentation using 2 L fermenter, we found that strains YKU 107, YKU 131 and YKU 132 was capable to hydrolyse 83.45%,67.45% and 71.9% of sago starch, respectively. However, only strain YKU 131 has the ability to produce significant amount of ethanol (2.16 gL-1) from sago starch. The superiority of strain YKU 131 as compared to strain YKU 107 and YKU 132 was found to be correlated with its ability to secrete glucoamylase. S. cerevisiae YKU 131 was selected for further studies. To investigate the performance of S. cerevlszae YKU 131 in ethanol production using various types of starch, initial starch concentration was fixed at 20 gL-1 Among different types of starch investigated, com starch was the most susceptible to the glucoamylase, followed by sago, cassava, potato and rice starch.
format	Thesis
qualification_level	Master's degree
author	Ang, Dek Chang
author_facet	Ang, Dek Chang
author_sort	Ang, Dek Chang
title	Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate
title_short	Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate
title_full	Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate
title_fullStr	Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate
title_full_unstemmed	Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate
title_sort	production of ethanol by genetically modified saccharomyces cerevisiae using sago starch as substrate
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Food Science and Biotechnology
publishDate	2001
url	http://psasir.upm.edu.my/id/eprint/11856/1/FSMB_2001_39.pdf
_version_	1804888660326219776

Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate

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