Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers

Retrotransposons are the most common class of eukaryotic transposable elements that make up over 50% of nuclear DNA found in many plants with large and complex genomes. The long terminal repeat (LTR) retrotransposons have a high degree of autonomy and encode at least five distinct protein compone...

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Main Author:	Teo, Chee How
Format:	Thesis
Language:	English English
Published:	2002
Subjects:	Bananas - Malaysia Food technology
Online Access:	http://psasir.upm.edu.my/id/eprint/11859/1/FSMB_2002_8_A.pdf
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id	my-upm-ir.11859
record_format	uketd_dc
spelling	my-upm-ir.118592011-07-19T06:54:54Z Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers 2002-04 Teo, Chee How Retrotransposons are the most common class of eukaryotic transposable elements that make up over 50% of nuclear DNA found in many plants with large and complex genomes. The long terminal repeat (LTR) retrotransposons have a high degree of autonomy and encode at least five distinct protein components, which are required for their movement in the genome. We have exploited the repetitive, dispersed nature of many LTR-containing retrotransposon families for the visualization of genomic polymorphism (genome constitution) and the visualization of varieties polymorphism (varieties classification). The IRAP-PCR (Inter- Retrotransposon Amplified Polymorphism-Polymerase Chain Reaction) markers are generated by the proximity of two LTRs or reverse transcriptase CRT) genes using outward-facing primers annealing to LTR target sequence. This method could distinguish between banana genome constitution (A and B genome) and varieties. This method can track new insertion of retrotransposon in banana tissue culture materials. The evolution of Malaysian bananas can also be tracked using this phylogenetic analysis. Since the method is PCR-based, it may allow the detection and isolation of the active, newly inserted retrotransposon copies easily, by analyzing the subsequent generations within an otherwise genetically homogeneous material. This method is applicable to other plants with dispersed families of LTR retrotransposons where either the LTR or nearby internal sequences are known. Bananas - Malaysia Food technology 2002-04 Thesis http://psasir.upm.edu.my/id/eprint/11859/ http://psasir.upm.edu.my/id/eprint/11859/1/FSMB_2002_8_A.pdf application/pdf en public masters Universiti Putra Malaysia Bananas - Malaysia Food technology Faculty of Food Science and Biotechnology English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Bananas - Malaysia Food technology
spellingShingle	Bananas - Malaysia Food technology Teo, Chee How Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers
description	Retrotransposons are the most common class of eukaryotic transposable elements that make up over 50% of nuclear DNA found in many plants with large and complex genomes. The long terminal repeat (LTR) retrotransposons have a high degree of autonomy and encode at least five distinct protein components, which are required for their movement in the genome. We have exploited the repetitive, dispersed nature of many LTR-containing retrotransposon families for the visualization of genomic polymorphism (genome constitution) and the visualization of varieties polymorphism (varieties classification). The IRAP-PCR (Inter- Retrotransposon Amplified Polymorphism-Polymerase Chain Reaction) markers are generated by the proximity of two LTRs or reverse transcriptase CRT) genes using outward-facing primers annealing to LTR target sequence. This method could distinguish between banana genome constitution (A and B genome) and varieties. This method can track new insertion of retrotransposon in banana tissue culture materials. The evolution of Malaysian bananas can also be tracked using this phylogenetic analysis. Since the method is PCR-based, it may allow the detection and isolation of the active, newly inserted retrotransposon copies easily, by analyzing the subsequent generations within an otherwise genetically homogeneous material. This method is applicable to other plants with dispersed families of LTR retrotransposons where either the LTR or nearby internal sequences are known.
format	Thesis
qualification_level	Master's degree
author	Teo, Chee How
author_facet	Teo, Chee How
author_sort	Teo, Chee How
title	Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers
title_short	Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers
title_full	Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers
title_fullStr	Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers
title_full_unstemmed	Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers
title_sort	classification of the malaysian banana varieties using retrotransposon markers
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Food Science and Biotechnology
publishDate	2002
url	http://psasir.upm.edu.my/id/eprint/11859/1/FSMB_2002_8_A.pdf
_version_	1747811274686726144

Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers

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