Evaluation Of Rapid Methods For Isolation And Characterization Of Salmonella Serovars Isolated From Raw Poultry And Vegetables
Cases of salmonellosis in humans have increased in recent years. Poultry, eggs, meat and dairy products are the most commonly implicated foods in salmonella food infection. The widespread increase in salmonellosis is of major health concern especially in the developing countries. The emergence of...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2003
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/11866/1/FSMB_2003_24.pdf |
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| Summary: | Cases of salmonellosis in humans have increased in recent years. Poultry, eggs, meat and
dairy products are the most commonly implicated foods in salmonella food infection. The
widespread increase in salmonellosis is of major health concern especially in the
developing countries. The emergence of S. Typbimurium DTI04 has further worsen the
problem because of its known multidrug-resistance. For the past three decades, S.
Typbimurium was the most frequently isolated serotype in Malaysia. However, in recent
studies S. Weltevreden were reported to be the most predominant serovar present in
foods. The present study examines the incidence of salmonellas in poultry and four types
of vegetables. However, the main focus of the study is to evaluate different rapid methods
for isolation and subsequently characterize the isolates obtained using various molecular
typing tools. The vegetables chosen for the study include 'selom' (Oenanthe stolonifera),
'pegaga' (Centella asiatica), 'kesum' (Polygonum minus) and 'kangkong' (Ipomoea
aquatica). Three hundred and sixty one Salmonella isolates were isolated from 157 samples of raw
poultry and the four types of vegetables. The study demonstrated that recoveries of
Salmonella were higher in poultry than in vegetables. Samples enriched in Rappaport
Vassiliadis (RV) broth and incubated at 420C gave higher recoveries compared to RV
broth at 3JOC, Mannitol Selenite Cysteine broth (SC) at 4rC and 37°C. Selective
enrichment in RV broth incubated at 42°C and subsequent plating on Hektoen Enteric
Agar (HEK) gave the highest number of Salmonella isolation from poultry and
vegetables samples. More Salmonella serotypes were isolated from samples enriched in
RV than from SC media while there was no obvious difference among Hektoen Enteric
Agar, Rambach Agar, Xylose Lysine Deoxycholate Agar and Bismuth Sulphite Agar
used for the recovery of Salmonella spp. The conventional cultural method gave highest recoveries of Salmonella followed by
enzyme-linked immunosorbent assay (ELISA) and immunomagnetic separation (lMS).
46.6% (34/73) of poultry and vegetable samples were positive for Salmonella by
conventional method, 26% (19/73) by ELISA and 1 7.8% ( 1 9/73) by IMS. Mixed growth
of diverse flora was observed on Rambach Agar, Hektoen Enteric Agar, Xylose Lysine
Deoxycholate Agar plates from IMS while growth of typical colonies of Salmonella were
observed on plates using conventional method. |
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