Chemical Constituents And Bioactivities Of Clausena Excavata
Clausena excavata (Rutaceae) which is locally known as “Pokok Kemantu”(ghostly tree) or “Pokok Cemamar” (diarrhoea tree) is one of the Malaysian species of ulam with high anti-oxidant properties. This plant is used in folk medicine for treating ailments like colic, cough, wounds, rhinitis, and st...
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| 格式: | Thesis |
| 語言: | English English |
| 出版: |
2009
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| 主題: | |
| 在線閱讀: | http://psasir.upm.edu.my/id/eprint/11968/1/FS_2009_36_A.pdf |
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| 總結: | Clausena excavata (Rutaceae) which is locally known as “Pokok Kemantu”(ghostly
tree) or “Pokok Cemamar” (diarrhoea tree) is one of the Malaysian species of ulam with
high anti-oxidant properties. This plant is used in folk medicine for treating ailments like
colic, cough, wounds, rhinitis, and stomach trouble, fever and as a tonic. Detail
investigations on the leaves, stem bark and roots of Clausena excavata (Rutaceae)
resulted in the isolation of nine compounds. These compounds are from several groups
of secondary metabolites such as limonoids, carbazole alkaloids, coumarins, and
triterpenes. The structures of these compounds were elucidated using spectroscopic
techniques such as NMR, GCMS, and IR and also by comparison with previously
reported data.The stem bark of Clausena excavata which was collected from Jabi and Pendang, Kedah
in December 2006 , yielded a new limonoid, clausenolide 1-methylether (133). This was
obtained from the methanol extract together with a known limonoid, clausenarin (8).
Besides that, two carbazole alkaloids, clausine–K (43) and 3-formyl-2,7-
dimethoxycarbazole (126) were isolated from the crude chloroform extract and two
triterpenes, stigmasterol (131) and β-sitosterol (132) were isolated from the hexane
extract. Similar work on the roots led to the isolation of three known coumarins which
were identified as xanthyletin (64) and dentatin (130), together with one carbazole
alkaloid, nordentatin (54).
The crude extracts and pure compounds of Clausena excavata were subjected to
cytotoxic activity test. The activities were expressed in terms of fifty percent inhibition
concentration (IC50) against HL60 cancer cell line (Human promyelocytic leukemia),
MCF-7 cancer cell line (Human breast cancer), HT-29 cancer cell line (Human colon
cancer), and HeLa cancer cell line. It was found that the crude hexane, chloroform, and
acetone extracts exhibited very strong activities against HL-60, and MCF-7 cell lines
with IC50 values < 6μg/mL. However, the new limonoid, clausenolide 1-methylether
(133) and clausenarin (8) which were isolated from the methanol extract of stem bark
showed no significant cytotoxicity against all the cancer cell lines with IC50 value
>30μg/mL as compared to another 2 isolated compounds, xanthyletin (64) and dentatin
(130) . |
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