Extraction Of y-Oryzanol And Tocopherols From Rice (Oryza Sativa) Bran

The objective of this study was to extract y-oryzanol and tocopherols from rice bran. Different extraction times and sequential extractions utilizing different solvents on y- oryzanol and tocopherols levels from crude rice bran oil (CRBO) were studied. Rice bran was extracted using hexane with fi...

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Main Author:	Ismail, Rosnizam
Format:	Thesis
Language:	English English
Published:	2008
Subjects:	Extraction (Chemistry) Rice bran
Online Access:	http://psasir.upm.edu.my/id/eprint/12001/1/FSTM_2008_19_A.pdf
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id	my-upm-ir.12001
record_format	uketd_dc
spelling	my-upm-ir.120012013-05-27T07:50:43Z Extraction Of y-Oryzanol And Tocopherols From Rice (Oryza Sativa) Bran 2008-07 Ismail, Rosnizam The objective of this study was to extract y-oryzanol and tocopherols from rice bran. Different extraction times and sequential extractions utilizing different solvents on y- oryzanol and tocopherols levels from crude rice bran oil (CRBO) were studied. Rice bran was extracted using hexane with five different extraction times, while sequential extractions was carried out utilizing hexane, hexane/dichloromethane, dichloromethane and dichloromethane/methanol. Extraction of y-oryzanol and tocopherols was further studied using precipitation and liquid-liquid separation from unsaponified fraction of CRBO. Precipitation was carried out by diluting the sample in hexane overnight at -20˚C. Liquid-liquid separations were carried out using hexanemethanol or hexane-acetonitrile. Solid and liquid portions formed from precipitation were further separated by liquid-liquid separations. The final stage of the extraction process was a combination of precipitation and single-stage chromatographic separation. Results showed that percentage of extracted oil was not significantly different with the different extraction times used. However, 20 min extraction resulted in the highest y-oryzanol level (p<0.05). On the other hand, 30 min extraction gave the highest tocopherols level (p<0.05). Sequential extractions resulted in higher y- oryzanol and tocopherols levels than that of hexane extraction alone (p<0.05). After saponification of CRBO extraction (with hexane, hexane/dichoromethane and dichloromethane), the unsaponified fraction was found to contain 1.4% y-oryzanol and 0.02% tocopherols. In liquid-liquid separations on unsaponified fraction of CRBO study, the process was able to concentrate 2.1% y-oryzanol in methanol and 1.8% in acetonitrile, respectively. On the other hand, 0.03% tocopherols was concentrated in methanol and 0.04% in acetonitrile, respectively. In precipitation study, 4.04% y- oryzanol was concentrated in solid portion. In column chromatography study, combining precipitation and single stage silica chromatography was able to concentrate 10.99% y-oryzanol and 0.02% tocopherols. This work proposed an alternative method for y-oryzanol and tocopherols extraction from rice bran. Extraction (Chemistry) Rice bran 2008-07 Thesis http://psasir.upm.edu.my/id/eprint/12001/ http://psasir.upm.edu.my/id/eprint/12001/1/FSTM_2008_19_A.pdf application/pdf en public masters Universiti Putra Malaysia Extraction (Chemistry) Rice bran Faculty of Food Science and Technology English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Extraction (Chemistry) Rice bran
spellingShingle	Extraction (Chemistry) Rice bran Ismail, Rosnizam Extraction Of y-Oryzanol And Tocopherols From Rice (Oryza Sativa) Bran
description	The objective of this study was to extract y-oryzanol and tocopherols from rice bran. Different extraction times and sequential extractions utilizing different solvents on y- oryzanol and tocopherols levels from crude rice bran oil (CRBO) were studied. Rice bran was extracted using hexane with five different extraction times, while sequential extractions was carried out utilizing hexane, hexane/dichloromethane, dichloromethane and dichloromethane/methanol. Extraction of y-oryzanol and tocopherols was further studied using precipitation and liquid-liquid separation from unsaponified fraction of CRBO. Precipitation was carried out by diluting the sample in hexane overnight at -20˚C. Liquid-liquid separations were carried out using hexanemethanol or hexane-acetonitrile. Solid and liquid portions formed from precipitation were further separated by liquid-liquid separations. The final stage of the extraction process was a combination of precipitation and single-stage chromatographic separation. Results showed that percentage of extracted oil was not significantly different with the different extraction times used. However, 20 min extraction resulted in the highest y-oryzanol level (p<0.05). On the other hand, 30 min extraction gave the highest tocopherols level (p<0.05). Sequential extractions resulted in higher y- oryzanol and tocopherols levels than that of hexane extraction alone (p<0.05). After saponification of CRBO extraction (with hexane, hexane/dichoromethane and dichloromethane), the unsaponified fraction was found to contain 1.4% y-oryzanol and 0.02% tocopherols. In liquid-liquid separations on unsaponified fraction of CRBO study, the process was able to concentrate 2.1% y-oryzanol in methanol and 1.8% in acetonitrile, respectively. On the other hand, 0.03% tocopherols was concentrated in methanol and 0.04% in acetonitrile, respectively. In precipitation study, 4.04% y- oryzanol was concentrated in solid portion. In column chromatography study, combining precipitation and single stage silica chromatography was able to concentrate 10.99% y-oryzanol and 0.02% tocopherols. This work proposed an alternative method for y-oryzanol and tocopherols extraction from rice bran.
format	Thesis
qualification_level	Master's degree
author	Ismail, Rosnizam
author_facet	Ismail, Rosnizam
author_sort	Ismail, Rosnizam
title	Extraction Of y-Oryzanol And Tocopherols From Rice (Oryza Sativa) Bran
title_short	Extraction Of y-Oryzanol And Tocopherols From Rice (Oryza Sativa) Bran
title_full	Extraction Of y-Oryzanol And Tocopherols From Rice (Oryza Sativa) Bran
title_fullStr	Extraction Of y-Oryzanol And Tocopherols From Rice (Oryza Sativa) Bran
title_full_unstemmed	Extraction Of y-Oryzanol And Tocopherols From Rice (Oryza Sativa) Bran
title_sort	extraction of y-oryzanol and tocopherols from rice (oryza sativa) bran
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Food Science and Technology
publishDate	2008
url	http://psasir.upm.edu.my/id/eprint/12001/1/FSTM_2008_19_A.pdf
_version_	1747811291014103040

Extraction Of y-Oryzanol And Tocopherols From Rice (Oryza Sativa) Bran

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