Effect Of Cooking Methods And Conditions On Heterocyclic Amines Content In Satay And Roasted Marinated Chicken
The objectives of this study were to determine the effect of cooking method on HAs concentration of chicken and beef satay and the effect of marinating and different cooking conditions on HAs concentration of roasted chicken. Six common HAs were investigated: 2-amino-3-methylimidazo[4,5-f]quinoli...
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Format: | Thesis |
Language: | English English |
Published: |
2009
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Subjects: | |
Online Access: | http://psasir.upm.edu.my/id/eprint/12007/1/FSTM_2009_30_A.pdf |
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Summary: | The objectives of this study were to determine the effect of cooking method on
HAs concentration of chicken and beef satay and the effect of marinating and
different cooking conditions on HAs concentration of roasted chicken. Six
common HAs were investigated: 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-
amino 3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), 2-amino-3,8-dimethylimidazo[
4,5-f]quinoxaline (MeIQx), 2-amino-3,4,8-trimethylimidazo[4,5-
f]quinoxaline (4,8-DiMeIQx), 2-amino-3,7,8-trimethylimidazo[4,5-f]quinoxaline
(7,8-DiMeIQx), 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP).
Samples were extracted using 1M NaOH and ethyl acetate and clean up on solid
phase extraction (SPE) column. The samples were then analysed using high
performance liquid chromatography (HPLC) equipped with photodiode-array
detector (DAD). Chicken and beef satay were grilled to two different degrees of
doneness (medium and well done). Three types of cooking method were applied UPLOAD to both types of satay i.e. charcoal grilled (treatment A), microwave pretreatment
prior to grilling (treatment B), and deep fried-microwave (treatment C). Both
chicken and beef satay samples which undergone microwave pretreatment prior
to grilling (treatment B) showed significantly (p<0.05) lower HAs concentration as
compared to charcoal grilled satay (treatment A). Deep fried-microwave
(treatment C) was applied to both types of satay as an alternative method to cook
satay and was proven to produce lesser HAs as compared to treatments A and B
in medium and well done cooked satay. HAs concentration were compared in
marinated and unmarinated chicken before roasting. Three roasting conditions
were applied to chicken i.e. 160°C for 120 min (treatment X), 180°C for 90 min
(treatment Y) and 200°C for 60 min (treatment Z). The study showed that
marinated chicken produced significantly (p<0.05) lower HAs concentration as
compared to unmarinated chicken in all three different cooking treatments.
Meanwhile, roasting at 160°C for 120 min (treatment X) was found to produce the
lowest HAs as compared to the other two treatments, 180°C for 90 min
(treatment Y) and 200°C for 60 min (treatment Z). Marinating the roasted chicken
with percik sauce has been shown to produce the lowest HAs concentration
when the samples were exposed to 200°C for 60 min (treatment Z) as compared
to other marinating sauces used in this study. These results revealed that
marinating chicken before roasting with various spices and herbs which contain
rich amount of antioxidants may reduce significantly the formation of mutagenic /
carcinogenic HAs in the products. |
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