Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase

Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase

The fed-batch cultivation process for the production of the recombinant protein, superoxide dismutase (SOD) by E. coli BL21 (DE3) pLysS was carried out. The cultivation process for recombinant SOD (ESOD) production was optimized through several approaches and strategies. The first approach was to...

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Main Author: Wan Omar, Wan Siti Atikah
Format: Thesis
Language:English
English
Published: 2009
Subjects:
Cultivation
Escherichia coli
Superoxide dismutase
Online Access:http://psasir.upm.edu.my/id/eprint/12060/1/IB_2009_17_A.pdf
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spelling my-upm-ir.120602013-05-27T07:50:57Z Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase 2009-10 Wan Omar, Wan Siti Atikah The fed-batch cultivation process for the production of the recombinant protein, superoxide dismutase (SOD) by E. coli BL21 (DE3) pLysS was carried out. The cultivation process for recombinant SOD (ESOD) production was optimized through several approaches and strategies. The first approach was to optimize medium and culture conditions of the ESOD culture in shake flask via conventional and the Response Surface Methodology (RSM) methods. Laboratory scale batch cultivation of ESOD was then performed using a 2 L stirred tank bioreactor (STB) in order to further optimize the medium and culture conditions. The effects of glucose concentrations (15 and 20 g/L), agitation speeds (300 – 1000 rpm) and controlled dissolved oxygen tension (DOT) via agitation speeds (20%, 50% and 80%) on the growth performance of the recombinant E. coli strain were investigated. In the final stage, fed-batch techniques were applied to the process for the development of high cell density cultivation. The performance and kinetics of the ESOD fed-batch and batch cultivations were then evaluated and compared. Cultivation Escherichia coli Superoxide dismutase 2009-10 Thesis http://psasir.upm.edu.my/id/eprint/12060/ http://psasir.upm.edu.my/id/eprint/12060/1/IB_2009_17_A.pdf application/pdf en public masters Universiti Putra Malaysia Cultivation Escherichia coli Superoxide dismutase Institute of Bioscience English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
topic Cultivation
Escherichia coli
Superoxide dismutase
spellingShingle Cultivation
Escherichia coli
Superoxide dismutase
Wan Omar, Wan Siti Atikah
Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase
description The fed-batch cultivation process for the production of the recombinant protein, superoxide dismutase (SOD) by E. coli BL21 (DE3) pLysS was carried out. The cultivation process for recombinant SOD (ESOD) production was optimized through several approaches and strategies. The first approach was to optimize medium and culture conditions of the ESOD culture in shake flask via conventional and the Response Surface Methodology (RSM) methods. Laboratory scale batch cultivation of ESOD was then performed using a 2 L stirred tank bioreactor (STB) in order to further optimize the medium and culture conditions. The effects of glucose concentrations (15 and 20 g/L), agitation speeds (300 – 1000 rpm) and controlled dissolved oxygen tension (DOT) via agitation speeds (20%, 50% and 80%) on the growth performance of the recombinant E. coli strain were investigated. In the final stage, fed-batch techniques were applied to the process for the development of high cell density cultivation. The performance and kinetics of the ESOD fed-batch and batch cultivations were then evaluated and compared.
format Thesis
qualification_level Master's degree
author Wan Omar, Wan Siti Atikah
author_facet Wan Omar, Wan Siti Atikah
author_sort Wan Omar, Wan Siti Atikah
title Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase
title_short Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase
title_full Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase
title_fullStr Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase
title_full_unstemmed Development Of Fed-Batch Cultivation Process For Esherichia Coli Harboring Superoxide Dismutase
title_sort development of fed-batch cultivation process for esherichia coli harboring superoxide dismutase
granting_institution Universiti Putra Malaysia
granting_department Institute of Bioscience
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/12060/1/IB_2009_17_A.pdf
_version_ 1747811296659636224

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