Isolation and Characterisation of Dichelobacter Nodosus from Footrot Infected Sheep in Malaysia

Twelve Dichelobacter nodosus were isolated from 12 sheep with footrot with lesion score 2. The isolates were studied and the results analysed. Diagnosis was done successfully by Gram-stain method while polymerase chain reaction (PCR) method with species specific primers, A and Ac were employed for s...

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Main Author:	Zakaria, Zunita
Format:	Thesis
Language:	English English
Published:	1998
Subjects:	Footrot in sheep - Malaysia Sheep - Infections - Malaysia
Online Access:	http://psasir.upm.edu.my/id/eprint/12179/1/FPV_1998_10_A.pdf
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id	my-upm-ir.12179
record_format	uketd_dc
spelling	my-upm-ir.121792014-10-28T02:16:20Z Isolation and Characterisation of Dichelobacter Nodosus from Footrot Infected Sheep in Malaysia 1998-03 Zakaria, Zunita Twelve Dichelobacter nodosus were isolated from 12 sheep with footrot with lesion score 2. The isolates were studied and the results analysed. Diagnosis was done successfully by Gram-stain method while polymerase chain reaction (PCR) method with species specific primers, A and Ac were employed for species confirmation. All 12 isolates reacted positively in the peR method by producing a single product of approximately 780 basepairs. All isolates, although obtained from distant locations, were from serogroup B (10 isolates were B2 serotype, 2 isolates were B1 serotype). The E-test method was used to determine the minimum inhibition concentration (MIC) values of nine antimicrobial agents against all 12 isolates. Penicillin G proved to be the most effective antibiotic with MIC90% of 0.023 ug/ml. Two standard conventional methods, the elastase and gelatin gel tests, were used in assessing the virulence of the isolates. Generally, the isolates exhibited variation in the laboratory characteristics although they had been isolated from similar lesion score. Some of the isolates which appeared to have the capability of causing virulent footrot in-vitro, failed to show clinical signs of virulent form of footrot. This was probably due to the frequent topical regimen adhered to and result of the vaccination programme by the farm management. All isolates were found not to contain plasmid by standard plasmid extracting method. This indicates that the genes coding for virulence of the isolates were not plasmidmediated. Molecular typing of the isolates was successfully carried out by pulsed field gel electrophoresis (PFGE) analysis. Significant patterns were generated by three GC-rich enzymes (ApaI, SfiI and SmaI) discriminating the isolates into eight genome types. Isolates from the same flock were also shown to possess variation in their PFGE profiles. These results demonstrate the diversity of D. nodosus strains infecting sheep in Malaysia and also indicated that the isolates were from diverse sources. Footrot in sheep - Malaysia Sheep - Infections - Malaysia 1998-03 Thesis http://psasir.upm.edu.my/id/eprint/12179/ http://psasir.upm.edu.my/id/eprint/12179/1/FPV_1998_10_A.pdf application/pdf en public masters Universiti Putra Malaysia Footrot in sheep - Malaysia Sheep - Infections - Malaysia Faculty of Veterinary Medicine English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Footrot in sheep - Malaysia Sheep - Infections - Malaysia
spellingShingle	Footrot in sheep - Malaysia Sheep - Infections - Malaysia Zakaria, Zunita Isolation and Characterisation of Dichelobacter Nodosus from Footrot Infected Sheep in Malaysia
description	Twelve Dichelobacter nodosus were isolated from 12 sheep with footrot with lesion score 2. The isolates were studied and the results analysed. Diagnosis was done successfully by Gram-stain method while polymerase chain reaction (PCR) method with species specific primers, A and Ac were employed for species confirmation. All 12 isolates reacted positively in the peR method by producing a single product of approximately 780 basepairs. All isolates, although obtained from distant locations, were from serogroup B (10 isolates were B2 serotype, 2 isolates were B1 serotype). The E-test method was used to determine the minimum inhibition concentration (MIC) values of nine antimicrobial agents against all 12 isolates. Penicillin G proved to be the most effective antibiotic with MIC90% of 0.023 ug/ml. Two standard conventional methods, the elastase and gelatin gel tests, were used in assessing the virulence of the isolates. Generally, the isolates exhibited variation in the laboratory characteristics although they had been isolated from similar lesion score. Some of the isolates which appeared to have the capability of causing virulent footrot in-vitro, failed to show clinical signs of virulent form of footrot. This was probably due to the frequent topical regimen adhered to and result of the vaccination programme by the farm management. All isolates were found not to contain plasmid by standard plasmid extracting method. This indicates that the genes coding for virulence of the isolates were not plasmidmediated. Molecular typing of the isolates was successfully carried out by pulsed field gel electrophoresis (PFGE) analysis. Significant patterns were generated by three GC-rich enzymes (ApaI, SfiI and SmaI) discriminating the isolates into eight genome types. Isolates from the same flock were also shown to possess variation in their PFGE profiles. These results demonstrate the diversity of D. nodosus strains infecting sheep in Malaysia and also indicated that the isolates were from diverse sources.
format	Thesis
qualification_level	Master's degree
author	Zakaria, Zunita
author_facet	Zakaria, Zunita
author_sort	Zakaria, Zunita
title	Isolation and Characterisation of Dichelobacter Nodosus from Footrot Infected Sheep in Malaysia
title_short	Isolation and Characterisation of Dichelobacter Nodosus from Footrot Infected Sheep in Malaysia
title_full	Isolation and Characterisation of Dichelobacter Nodosus from Footrot Infected Sheep in Malaysia
title_fullStr	Isolation and Characterisation of Dichelobacter Nodosus from Footrot Infected Sheep in Malaysia
title_full_unstemmed	Isolation and Characterisation of Dichelobacter Nodosus from Footrot Infected Sheep in Malaysia
title_sort	isolation and characterisation of dichelobacter nodosus from footrot infected sheep in malaysia
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Veterinary Medicine
publishDate	1998
url	http://psasir.upm.edu.my/id/eprint/12179/1/FPV_1998_10_A.pdf
_version_	1747811322315145216

Isolation and Characterisation of Dichelobacter Nodosus from Footrot Infected Sheep in Malaysia

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