In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats

Pneumonic pasteurellosis is an important respiratory disease of sheep and goats throughout the world. It is mainly caused by Pasteurella haemolytica even though Pasteurella multocida has occasionally been associated with the same disease. In vitro challenge of lung tissues with Pasteurella haemolyt...

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Main Author: Mat Amin, Maswati
Format: Thesis
Language:English
English
Published: 1998
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Online Access:http://psasir.upm.edu.my/id/eprint/12187/1/FPV_1998_5_A.pdf
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spelling my-upm-ir.121872011-09-07T02:15:17Z In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats 1998-07 Mat Amin, Maswati Pneumonic pasteurellosis is an important respiratory disease of sheep and goats throughout the world. It is mainly caused by Pasteurella haemolytica even though Pasteurella multocida has occasionally been associated with the same disease. In vitro challenge of lung tissues with Pasteurella haemolytica A2 revealed an early colonization of the bacteria onto the lung tissue as early as 1 hour post-challenge. The severity of colonization increased with time of challenge and reached a maximum rate at 6 hours post-challenge . Similar in vitro challenge on the lung tissues derived from goats that were exposed earlier to intranasal sprays of formalin-killed Pasteurella haemolytica A2, however, revealed a less severe colonization by 6 hours postchallenge. Following intratracheal challenge of goats with 108/ml colony forming units of Pasteurella haemolytica A2, 20% of the goats succumbed to peracute infection in which they died within 12 hours post-challenge. Examinations of the lungs revealed classical toxaemic lesions consisted of severe pulmonary oedema, pulmonary congestion and haemorrhage, and thrombosis with a few neutrophils in the alveoli. The lesions were remarkably similar to those peracute infections caused by Pasteurella multocida types A and D. The only difference was the absence of Pasteurella haemolytica A2 organisms in the heart blood samples compared to the infections by Pasteurella multocida types A and D. Goats that survived the peracute episode developed pneumonic lesion. Phagocytic activity by the bronchoalveolar macrophages was obvious by 4 days postchallenge and by day 7 post-challenge, the goats which were unable to phagocytose most of the bacterial cells succumbed to severe pneumonia in which the bacteria proliferated and overloaded the lungs leading to the invasion of the bacteria into the pneumocytes and spreading the infection further. Goats with efficient phagocytosis were able to reduce the number of bacterial cells in the lungs leading to failure of bacterial establishment in the lungs. Infections by Pasteurella haemolytica A2 isolated from nasal mucosa produced an insignificantly (p>0.05) less extensive lung lesions compared to infections by Pasteurella haemolytica A2 isolated from pneumonic lungs. The pulmonary responses and pattern of lesion developrrient, however, remained similar for both isolates. Goats - Diseases Sheep - Diseases 1998-07 Thesis http://psasir.upm.edu.my/id/eprint/12187/ http://psasir.upm.edu.my/id/eprint/12187/1/FPV_1998_5_A.pdf application/pdf en public masters Universiti Putra Malaysia Goats - Diseases Sheep - Diseases Faculty of Veterinary Medicine English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
topic Goats - Diseases
Sheep - Diseases

spellingShingle Goats - Diseases
Sheep - Diseases

Mat Amin, Maswati
In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats
description Pneumonic pasteurellosis is an important respiratory disease of sheep and goats throughout the world. It is mainly caused by Pasteurella haemolytica even though Pasteurella multocida has occasionally been associated with the same disease. In vitro challenge of lung tissues with Pasteurella haemolytica A2 revealed an early colonization of the bacteria onto the lung tissue as early as 1 hour post-challenge. The severity of colonization increased with time of challenge and reached a maximum rate at 6 hours post-challenge . Similar in vitro challenge on the lung tissues derived from goats that were exposed earlier to intranasal sprays of formalin-killed Pasteurella haemolytica A2, however, revealed a less severe colonization by 6 hours postchallenge. Following intratracheal challenge of goats with 108/ml colony forming units of Pasteurella haemolytica A2, 20% of the goats succumbed to peracute infection in which they died within 12 hours post-challenge. Examinations of the lungs revealed classical toxaemic lesions consisted of severe pulmonary oedema, pulmonary congestion and haemorrhage, and thrombosis with a few neutrophils in the alveoli. The lesions were remarkably similar to those peracute infections caused by Pasteurella multocida types A and D. The only difference was the absence of Pasteurella haemolytica A2 organisms in the heart blood samples compared to the infections by Pasteurella multocida types A and D. Goats that survived the peracute episode developed pneumonic lesion. Phagocytic activity by the bronchoalveolar macrophages was obvious by 4 days postchallenge and by day 7 post-challenge, the goats which were unable to phagocytose most of the bacterial cells succumbed to severe pneumonia in which the bacteria proliferated and overloaded the lungs leading to the invasion of the bacteria into the pneumocytes and spreading the infection further. Goats with efficient phagocytosis were able to reduce the number of bacterial cells in the lungs leading to failure of bacterial establishment in the lungs. Infections by Pasteurella haemolytica A2 isolated from nasal mucosa produced an insignificantly (p>0.05) less extensive lung lesions compared to infections by Pasteurella haemolytica A2 isolated from pneumonic lungs. The pulmonary responses and pattern of lesion developrrient, however, remained similar for both isolates.
format Thesis
qualification_level Master's degree
author Mat Amin, Maswati
author_facet Mat Amin, Maswati
author_sort Mat Amin, Maswati
title In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats
title_short In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats
title_full In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats
title_fullStr In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats
title_full_unstemmed In Vitro and In Vivo Establishment of Pasteurella Haemolytica A2 in the Lungs of Goats
title_sort in vitro and in vivo establishment of pasteurella haemolytica a2 in the lungs of goats
granting_institution Universiti Putra Malaysia
granting_department Faculty of Veterinary Medicine
publishDate 1998
url http://psasir.upm.edu.my/id/eprint/12187/1/FPV_1998_5_A.pdf
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