Cytogenetic Studies On The Water Buffalo (Bubalus Bubalis)
Chromosome analysis was undertaken on Murrah,swamp , F1 (Murrah x swamp ) and backcross (F1 female x Murrah male) buffaloes (Bubalus bubalis) using a modified leucocyte culture method combined with Giemsa (G) constitutive heterochromatin (C) and nucleolus organiser region (NOR) banding procedure...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1984
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/12263/1/FPV_1984_3_.pdf |
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| Summary: | Chromosome analysis was undertaken on Murrah,swamp , F1
(Murrah x swamp ) and backcross (F1 female x Murrah male) buffaloes
(Bubalus bubalis) using a modified leucocyte culture method
combined with Giemsa (G) constitutive heterochromatin (C) and
nucleolus organiser region (NOR) banding procedures . A combination
of 80% medium RPMI 1640 , 20% calf serum , pokeweed mitogen and buffy
coat gave many good quality metaphases . The Murrah , swamp and F1
buffaloes had chromosome complement s of 2n = 50 , 2n = 48 and
2n = 49 respectively . The Murrah karyotype had 5 pairs of submetacentric
chromosome s and 20 pairs of acrocentric chromosomes while
the swamp karyotype had 1 pair of large metacentric chromosomes , 4 submetacentric pairs and 19 pairs of acrocentric chromosomes. /F1
hybrid had an intermediate karyotype. Nine backcross animals had a
2n = 49 complement while 3 backcross animals had a 2n = 50 complement. |
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