A Study On A Velogenic Viscerotropic Newscastle Disease Virus In Vitro And In Vivo
The velogenic viscerotropic Newcastle disease virus or the Asiatic strain has been considered the most virulent strain of Newcastle disease virus. It is commonly found in Southeast Asia and it has been known to cause 100\ mortality in susceptible flocks. In spite of this, very little research ha...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
1985
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/12264/1/FPV_1985_1_.pdf |
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| Summary: | The velogenic viscerotropic Newcastle disease virus or the
Asiatic strain has been considered the most virulent strain of
Newcastle disease virus. It is commonly found in Southeast Asia
and it has been known to cause 100\ mortality in susceptible
flocks. In spite of this, very little research has been conducted
on it as many countries prohibit the handling of this strain of
virus. In view of this, a project has been undertaken at Universiti
Pertanian Malaysia to study the biological properties, cytopathogenicity
and morphogenesis of a locally isolated velogenic
viscerotropic Newcastle disease virus and to determine its
effects on the trachea of nonvaccinated and vaccinated chickens.
The in vitro study has shown that this virus has a mean
death time of 66 hours, and an intracerebral pathogenicity index
of 1.90 . Polykaryocytosis is the principal form of cytopathic
effect it produces in chick embryo fibroblasts and cells infected
by it haemadsorp red blood cells. This virus plaques in cell
culture. Negatively stained virus particles have diameters ranging from 100 to 600 nanometers. Electron microscopy demonstrated
that the virus replicates in the cytoplasm of infected
cells and aggregates of nucleocapsids are found in the cytoplasm.
The virus matures at the cell membrane and is released by
budding. |
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