Antimicrobial Resistance Of Escherichia Coli Isolates In Pig Farm Workers, Normal And Diarrhoeic Pigs
The enumeration of coliforms in faeces can be per formed by both the spread plate method or Most Probable Number ( MPN ) techniques , although MPN total coliform counts are significantly higher ( P<0.05 ) . In so lid agar media enumeration , MacConkey , Desoxycholate , Eosin-Methylene Blue an...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1991
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/12291/1/FPV_1991_1_A.pdf |
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| Summary: | The enumeration of coliforms in faeces can be per formed by
both the spread plate method or Most Probable Number ( MPN )
techniques , although MPN total coliform counts are significantly
higher ( P<0.05 ) . In so lid agar media enumeration , MacConkey ,
Desoxycholate , Eosin-Methylene Blue and ENDO agars are suitable
media for the enumeration of coliforms . The correlation
coefficients of counts among media and technique is high . The mean
colony forming unit ( CFU ) counts per gram for coliform in faeces
varied from 2 . 6-6 . 4 X 107 and 1 . 8-2 . 7 X 108 for the pigs and form
workers tested respectively .
Random identification of 291 colonies on MacConkey agar on the
basis of frequency of occurrence of numbers of lactose and nonlactose fermenters yielded 94 . 2 % Escherichia coli (E.coli).The
other genera isolated includes Enterobacter , Klebsiella ,
Edwarsiella , Citrobacter and Serratia . About 8.2 % and 29.6 % of the
E. coli isolates were non-lactose fermenters on MacConkey agar and
did not show the characteristic green metallic sheen on Eosin-
Methylene Blue agar respectively. |
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