Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes

A study on the characteristics of the Malaysian field and vaccine strains of Pasteurella multocida type B was conducted . In addition , the immunogenic potential of haemorrhagic septicaemia ( HS ) vaccines was evaluated in buffaloes . A total of 48 P. multocida isolates including the five HS va...

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Main Author:	Subramaniam, Chandrasekaran
Format:	Thesis
Language:	English English
Published:	1993
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Online Access:	http://psasir.upm.edu.my/id/eprint/12302/1/FPV_1993_1_A.pdf
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id	my-upm-ir.12302
record_format	uketd_dc
spelling	my-upm-ir.123022011-09-07T07:32:59Z Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes 1993-06 Subramaniam, Chandrasekaran A study on the characteristics of the Malaysian field and vaccine strains of Pasteurella multocida type B was conducted . In addition , the immunogenic potential of haemorrhagic septicaemia ( HS ) vaccines was evaluated in buffaloes . A total of 48 P. multocida isolates including the five HS vaccine strains were included in the study . Initial studies showed significant cross-protection in mice amongst the five vaccine strains . The 48 isolates , when subjected to biochemical tests had very little or no variability amongst them. Type-specific hyper immune sera were prepared in rabbits and buffaloes . The sera were then absorbed using antigens prepared from the 48 isolates . A series of serological tests were conducted using the pre- and post-absorbed sera and the antigen preparations . The protein profile of the five vaccine and five randomly selected field strains of this organism revealed that there appeared to be no variation amongst the field and vaccine strains . The antibody responses of buffaloes immunised with three conventional HS vaccines viz . , broth bacterin ( BB ) , alum-precipitated vaccine ( APV ) and oil adjuvant vaccine ( OAV ) and one experimental double emulsion vaccine ( DEV ) were determined by an enzyme-linked immunosorbent assay ( ELISA ) . Antibody levels were significantly higher in buffaloes immunised with the adjuvanted vaccines ( APV , OAV , DEV ) . Buffaloes immunised with BB alone were protected at 6 weeks but not at 12 weeks post-immunisation . Buffaloes immunised with APV were protected at 24 weeks but only partially at 52 weeks post-immunisation . On the other hand , buffaloes immunised with OAV or DEV were fully protected at 52 weeks but only partially at 76 weeks post-immunisation .There was also a positive relationship between the ELISA titres and protection in buffaloes . 1993-06 Thesis http://psasir.upm.edu.my/id/eprint/12302/ http://psasir.upm.edu.my/id/eprint/12302/1/FPV_1993_1_A.pdf application/pdf en public masters Universiti Pertanian Malaysia Faculty of Veterinary medicine English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic
spellingShingle	Subramaniam, Chandrasekaran Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes
description	A study on the characteristics of the Malaysian field and vaccine strains of Pasteurella multocida type B was conducted . In addition , the immunogenic potential of haemorrhagic septicaemia ( HS ) vaccines was evaluated in buffaloes . A total of 48 P. multocida isolates including the five HS vaccine strains were included in the study . Initial studies showed significant cross-protection in mice amongst the five vaccine strains . The 48 isolates , when subjected to biochemical tests had very little or no variability amongst them. Type-specific hyper immune sera were prepared in rabbits and buffaloes . The sera were then absorbed using antigens prepared from the 48 isolates . A series of serological tests were conducted using the pre- and post-absorbed sera and the antigen preparations . The protein profile of the five vaccine and five randomly selected field strains of this organism revealed that there appeared to be no variation amongst the field and vaccine strains . The antibody responses of buffaloes immunised with three conventional HS vaccines viz . , broth bacterin ( BB ) , alum-precipitated vaccine ( APV ) and oil adjuvant vaccine ( OAV ) and one experimental double emulsion vaccine ( DEV ) were determined by an enzyme-linked immunosorbent assay ( ELISA ) . Antibody levels were significantly higher in buffaloes immunised with the adjuvanted vaccines ( APV , OAV , DEV ) . Buffaloes immunised with BB alone were protected at 6 weeks but not at 12 weeks post-immunisation . Buffaloes immunised with APV were protected at 24 weeks but only partially at 52 weeks post-immunisation . On the other hand , buffaloes immunised with OAV or DEV were fully protected at 52 weeks but only partially at 76 weeks post-immunisation .There was also a positive relationship between the ELISA titres and protection in buffaloes .
format	Thesis
qualification_level	Master's degree
author	Subramaniam, Chandrasekaran
author_facet	Subramaniam, Chandrasekaran
author_sort	Subramaniam, Chandrasekaran
title	Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes
title_short	Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes
title_full	Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes
title_fullStr	Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes
title_full_unstemmed	Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes
title_sort	pasteurella multocida type b in malaysia: characterisation, immune response and protection in vaccinated buffaloes
granting_institution	Universiti Pertanian Malaysia
granting_department	Faculty of Veterinary medicine
publishDate	1993
url	http://psasir.upm.edu.my/id/eprint/12302/1/FPV_1993_1_A.pdf
_version_	1747811346293981184

Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes

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