Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers

A series of experiments were conducted to isolate and characterize suitable intestinal microbes as direct-fed microbials and to investigate their effects on broiler. and the mechanisms associated with their utilization. The major types of bacteria found in the duodenum and jeju-ileum of chicken...

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Main Author:	Jin, Lizhi
Format:	Thesis
Language:	English English
Published:	1996
Subjects:	Universiti Pertanian Malaysia - Dissertations Bacterial diseases in poultry Poultry - Infections
Online Access:	http://psasir.upm.edu.my/id/eprint/12319/1/FPV_1996_1_A.pdf
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id	my-upm-ir.12319
record_format	uketd_dc
spelling	my-upm-ir.123192011-09-12T01:24:16Z Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers 1996-05 Jin, Lizhi A series of experiments were conducted to isolate and characterize suitable intestinal microbes as direct-fed microbials and to investigate their effects on broiler. and the mechanisms associated with their utilization. The major types of bacteria found in the duodenum and jeju-ileum of chicken were Lactobacillus, Streptococcus and E. coli. In the cecum, ten groups of bacteria, namely, Streptococcus, Staphylococcus, Lactobacillus, E. coli, anaerobic coccus, Eubacterium, Propiombacterium, Clostridium, Fusobacterium and Bacteroides were isolated and identified. The main Lactobacillus species in chicken intestine were L. acidophilus, L. fermentum and L. brevis In vitro adhesion study demonstrated that 1 2 out of the 46 intestinal Lactobacillus isolates showed moderate to good ability to adhere to the ileal epithelial cell of chicken. Temperature (between 4°C and 42°C) did not have an effect on the attachment of the Lactobacillus isolates to the ileal epithelial cell. Incubation (contact) time of 30 min was found to be insufficient for the attachment of bacterial to the ileal epithelial cell, but contact time extended beyond 1 h did not improve the ability of the bacteria to adhere to the ileal epithelial cell. The pH values of suspending buffer for the Lactobacillus strains, ranging from 4 to 7, did not have any significant effect on the attachment of the bacteria to the ileal epithelial cell, but pH 8 reduced significantly (p<0.05) the attachment of bacteria. The growth of the 12 adherent Lactobacillus isolates was inhibited only slightly by the inclusion of 0.3% chicken bile in the culture medium, and they all showed a tolerance (in varying degrees) to low pH values ( 1.0 or 2.0). Universiti Pertanian Malaysia - Dissertations Bacterial diseases in poultry Poultry - Infections 1996-05 Thesis http://psasir.upm.edu.my/id/eprint/12319/ http://psasir.upm.edu.my/id/eprint/12319/1/FPV_1996_1_A.pdf application/pdf en public phd doctoral Universiti Pertanian Malaysia Universiti Pertanian Malaysia - Dissertations Bacterial diseases in poultry Poultry - Infections Faculty of Veterinary medicine English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Universiti Pertanian Malaysia - Dissertations Bacterial diseases in poultry Poultry - Infections
spellingShingle	Universiti Pertanian Malaysia - Dissertations Bacterial diseases in poultry Poultry - Infections Jin, Lizhi Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers
description	A series of experiments were conducted to isolate and characterize suitable intestinal microbes as direct-fed microbials and to investigate their effects on broiler. and the mechanisms associated with their utilization. The major types of bacteria found in the duodenum and jeju-ileum of chicken were Lactobacillus, Streptococcus and E. coli. In the cecum, ten groups of bacteria, namely, Streptococcus, Staphylococcus, Lactobacillus, E. coli, anaerobic coccus, Eubacterium, Propiombacterium, Clostridium, Fusobacterium and Bacteroides were isolated and identified. The main Lactobacillus species in chicken intestine were L. acidophilus, L. fermentum and L. brevis In vitro adhesion study demonstrated that 1 2 out of the 46 intestinal Lactobacillus isolates showed moderate to good ability to adhere to the ileal epithelial cell of chicken. Temperature (between 4°C and 42°C) did not have an effect on the attachment of the Lactobacillus isolates to the ileal epithelial cell. Incubation (contact) time of 30 min was found to be insufficient for the attachment of bacterial to the ileal epithelial cell, but contact time extended beyond 1 h did not improve the ability of the bacteria to adhere to the ileal epithelial cell. The pH values of suspending buffer for the Lactobacillus strains, ranging from 4 to 7, did not have any significant effect on the attachment of the bacteria to the ileal epithelial cell, but pH 8 reduced significantly (p<0.05) the attachment of bacteria. The growth of the 12 adherent Lactobacillus isolates was inhibited only slightly by the inclusion of 0.3% chicken bile in the culture medium, and they all showed a tolerance (in varying degrees) to low pH values ( 1.0 or 2.0).
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Jin, Lizhi
author_facet	Jin, Lizhi
author_sort	Jin, Lizhi
title	Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers
title_short	Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers
title_full	Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers
title_fullStr	Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers
title_full_unstemmed	Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers
title_sort	studies on the mechanisms and utilization of probiotics (direct-fed microbials) in broilers
granting_institution	Universiti Pertanian Malaysia
granting_department	Faculty of Veterinary medicine
publishDate	1996
url	http://psasir.upm.edu.my/id/eprint/12319/1/FPV_1996_1_A.pdf
_version_	1747811350445293568

Studies On The Mechanisms And Utilization Of Probiotics (Direct-Fed Microbials) In Broilers

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