Characterisation And Pathogenicity Studies Of Malaysian Isolates Of Avian Pox Virus
Avian pox is one of the important viral diseases in avian species of which, fowl pox is the most common disease and is usually controlled by vaccination. However, in recent years, outbreaks of the disease in vaccinated flocks have been reported. Therefore, it is important to study the local isola...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
1997
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/12326/1/FPV_1997_5_A.pdf |
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| Summary: | Avian pox is one of the important viral diseases in avian species of which,
fowl pox is the most common disease and is usually controlled by vaccination.
However, in recent years, outbreaks of the disease in vaccinated flocks have been
reported. Therefore, it is important to study the local isolates as well as vaccine
strains in order to determine the cause of the outbreaks.
This study was conducted to isolate, identify and characterise the field
strains of avipox virus, which caused outbreaks among poultry in Malaysia. The
isolates were also compared with the vaccine strain (TC-B I2) by pathogenicity
studies in chickens. Initial characterisation of isolates involved morphology by
electron microscopy, changes in embryonated chicken eggs, inclusion body
formation, immunoperoxidase test and cytopathogenicity in cell culture.Five avipox viruses were isolated from chicken, pigeon and turkey.
Negative contrast electron microscopy showed the unique morphology of the
isolates and did not show an y differentiation among the isolates in respect to their
origin. The isolates were titrated in embryonated eggs and the isolates from pigeon
and turkey gave lower titre in comparison to other isolates. On chorioallantoic
membrane (CAM), mother pock lesions were produced by the isolates from pigeon
and turkey in comparison to the isolates from chicken which produced scattered
pock lesions. |
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