Characterisation And Pathogenicity Studies Of Malaysian Isolates Of Avian Pox Virus

Avian pox is one of the important viral diseases in avian species of which, fowl pox is the most common disease and is usually controlled by vaccination. However, in recent years, outbreaks of the disease in vaccinated flocks have been reported. Therefore, it is important to study the local isola...

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Main Author:	Sivasothy, Arumugam
Format:	Thesis
Language:	English English
Published:	1997
Subjects:	Fowl pox - Viruses - Malaysi Poultry - Virus diseases - Malaysia Poxvirus diseases - Malaysia
Online Access:	http://psasir.upm.edu.my/id/eprint/12326/1/FPV_1997_5_A.pdf
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id	my-upm-ir.12326
record_format	uketd_dc
spelling	my-upm-ir.123262011-07-13T01:26:15Z Characterisation And Pathogenicity Studies Of Malaysian Isolates Of Avian Pox Virus 1997-04 Sivasothy, Arumugam Avian pox is one of the important viral diseases in avian species of which, fowl pox is the most common disease and is usually controlled by vaccination. However, in recent years, outbreaks of the disease in vaccinated flocks have been reported. Therefore, it is important to study the local isolates as well as vaccine strains in order to determine the cause of the outbreaks. This study was conducted to isolate, identify and characterise the field strains of avipox virus, which caused outbreaks among poultry in Malaysia. The isolates were also compared with the vaccine strain (TC-B I2) by pathogenicity studies in chickens. Initial characterisation of isolates involved morphology by electron microscopy, changes in embryonated chicken eggs, inclusion body formation, immunoperoxidase test and cytopathogenicity in cell culture.Five avipox viruses were isolated from chicken, pigeon and turkey. Negative contrast electron microscopy showed the unique morphology of the isolates and did not show an y differentiation among the isolates in respect to their origin. The isolates were titrated in embryonated eggs and the isolates from pigeon and turkey gave lower titre in comparison to other isolates. On chorioallantoic membrane (CAM), mother pock lesions were produced by the isolates from pigeon and turkey in comparison to the isolates from chicken which produced scattered pock lesions. Fowl pox - Viruses - Malaysi Poultry - Virus diseases - Malaysia Poxvirus diseases - Malaysia 1997-04 Thesis http://psasir.upm.edu.my/id/eprint/12326/ http://psasir.upm.edu.my/id/eprint/12326/1/FPV_1997_5_A.pdf application/pdf en public masters Universiti Putra Malaysia Fowl pox - Viruses - Malaysi Poultry - Virus diseases - Malaysia Poxvirus diseases - Malaysia Faculty of Veterinary medicine English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Fowl pox - Viruses - Malaysi Poultry - Virus diseases - Malaysia Poxvirus diseases - Malaysia
spellingShingle	Fowl pox - Viruses - Malaysi Poultry - Virus diseases - Malaysia Poxvirus diseases - Malaysia Sivasothy, Arumugam Characterisation And Pathogenicity Studies Of Malaysian Isolates Of Avian Pox Virus
description	Avian pox is one of the important viral diseases in avian species of which, fowl pox is the most common disease and is usually controlled by vaccination. However, in recent years, outbreaks of the disease in vaccinated flocks have been reported. Therefore, it is important to study the local isolates as well as vaccine strains in order to determine the cause of the outbreaks. This study was conducted to isolate, identify and characterise the field strains of avipox virus, which caused outbreaks among poultry in Malaysia. The isolates were also compared with the vaccine strain (TC-B I2) by pathogenicity studies in chickens. Initial characterisation of isolates involved morphology by electron microscopy, changes in embryonated chicken eggs, inclusion body formation, immunoperoxidase test and cytopathogenicity in cell culture.Five avipox viruses were isolated from chicken, pigeon and turkey. Negative contrast electron microscopy showed the unique morphology of the isolates and did not show an y differentiation among the isolates in respect to their origin. The isolates were titrated in embryonated eggs and the isolates from pigeon and turkey gave lower titre in comparison to other isolates. On chorioallantoic membrane (CAM), mother pock lesions were produced by the isolates from pigeon and turkey in comparison to the isolates from chicken which produced scattered pock lesions.
format	Thesis
qualification_level	Master's degree
author	Sivasothy, Arumugam
author_facet	Sivasothy, Arumugam
author_sort	Sivasothy, Arumugam
title	Characterisation And Pathogenicity Studies Of Malaysian Isolates Of Avian Pox Virus
title_short	Characterisation And Pathogenicity Studies Of Malaysian Isolates Of Avian Pox Virus
title_full	Characterisation And Pathogenicity Studies Of Malaysian Isolates Of Avian Pox Virus
title_fullStr	Characterisation And Pathogenicity Studies Of Malaysian Isolates Of Avian Pox Virus
title_full_unstemmed	Characterisation And Pathogenicity Studies Of Malaysian Isolates Of Avian Pox Virus
title_sort	characterisation and pathogenicity studies of malaysian isolates of avian pox virus
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Veterinary medicine
publishDate	1997
url	http://psasir.upm.edu.my/id/eprint/12326/1/FPV_1997_5_A.pdf
_version_	1747811352157618176

Characterisation And Pathogenicity Studies Of Malaysian Isolates Of Avian Pox Virus

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