Detection Of Triglyceride Based On Microwave Dielectric Properties Of Triglyceride Enzymatic Reaction

Triglyceride is identified to have an important function in dairy products since it can cause milk rancidity and affect cheese flavor. In this study, the detection of triglyceride was done through the measurement of dielectric properties of triglyceride enzymatic reaction in the frequencies rangi...

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Main Author:	Hamid, Hasnidar
Format:	Thesis
Language:	English English
Published:	2010
Subjects:	Triglycerides - Analysis Dielectrics Microwaves
Online Access:	http://psasir.upm.edu.my/id/eprint/12361/1/FS_2010_18A.pdf
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id	my-upm-ir.12361
record_format	uketd_dc
spelling	my-upm-ir.123612013-05-27T07:51:51Z Detection Of Triglyceride Based On Microwave Dielectric Properties Of Triglyceride Enzymatic Reaction 2010-07 Hamid, Hasnidar Triglyceride is identified to have an important function in dairy products since it can cause milk rancidity and affect cheese flavor. In this study, the detection of triglyceride was done through the measurement of dielectric properties of triglyceride enzymatic reaction in the frequencies ranging from 200 MHz to 20 GHz by using Network Analyzer. This measurement is suitable to investigate the changes in dielectric properties of triglyceride and furthermore the glycerol and fatty acids formed from this reaction. The dielectric properties measured are dielectric constant, ε’ and dielectric loss, ε”. These dielectric properties are important in carrying out the investigation of molecules interaction of triglyceride as a substrate and lipase as a catalyst in the enzymatic reaction while irradiated with the electromagnetic field. This interaction can detect the presence of ionic conductivity at low frequency and also dipole orientation at high frequency of microwaves frequency range. From the interaction of triglyceride with the presence of lipase as a catalyst, the expected products obtained are fatty acids and glycerol. The glycerol and fatty acids formed from the reaction that can be seen as a cloudy upper layer liquid and a clear lower layer which can be observed after a stable condition at about 4 minutes after the reaction. By analyzing the dielectric properties of upper layer with the dielectric properties of the enzyme, a good relationship can be obtained between these values to the triglyceride concentration. For better sensitivity of the measurement, the chosen frequencies of the measurement are about 1 GHz, 2.6 GHz, 10 GHz and 15 GHz and the preferable mixing ratio between substrate and enzyme is 1:1. This condition is applied to the triglyceride and milks samples of concentration from 10000 ppm to 50000 ppm. The reading of dielectric properties was found to increase with the increasing concentration of the triglyceride and milk samples. Triglycerides - Analysis Dielectrics Microwaves 2010-07 Thesis http://psasir.upm.edu.my/id/eprint/12361/ http://psasir.upm.edu.my/id/eprint/12361/1/FS_2010_18A.pdf application/pdf en public masters Universiti Putra Malaysia Triglycerides - Analysis Dielectrics Microwaves Faculty Of Science English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Triglycerides - Analysis Dielectrics Microwaves
spellingShingle	Triglycerides - Analysis Dielectrics Microwaves Hamid, Hasnidar Detection Of Triglyceride Based On Microwave Dielectric Properties Of Triglyceride Enzymatic Reaction
description	Triglyceride is identified to have an important function in dairy products since it can cause milk rancidity and affect cheese flavor. In this study, the detection of triglyceride was done through the measurement of dielectric properties of triglyceride enzymatic reaction in the frequencies ranging from 200 MHz to 20 GHz by using Network Analyzer. This measurement is suitable to investigate the changes in dielectric properties of triglyceride and furthermore the glycerol and fatty acids formed from this reaction. The dielectric properties measured are dielectric constant, ε’ and dielectric loss, ε”. These dielectric properties are important in carrying out the investigation of molecules interaction of triglyceride as a substrate and lipase as a catalyst in the enzymatic reaction while irradiated with the electromagnetic field. This interaction can detect the presence of ionic conductivity at low frequency and also dipole orientation at high frequency of microwaves frequency range. From the interaction of triglyceride with the presence of lipase as a catalyst, the expected products obtained are fatty acids and glycerol. The glycerol and fatty acids formed from the reaction that can be seen as a cloudy upper layer liquid and a clear lower layer which can be observed after a stable condition at about 4 minutes after the reaction. By analyzing the dielectric properties of upper layer with the dielectric properties of the enzyme, a good relationship can be obtained between these values to the triglyceride concentration. For better sensitivity of the measurement, the chosen frequencies of the measurement are about 1 GHz, 2.6 GHz, 10 GHz and 15 GHz and the preferable mixing ratio between substrate and enzyme is 1:1. This condition is applied to the triglyceride and milks samples of concentration from 10000 ppm to 50000 ppm. The reading of dielectric properties was found to increase with the increasing concentration of the triglyceride and milk samples.
format	Thesis
qualification_level	Master's degree
author	Hamid, Hasnidar
author_facet	Hamid, Hasnidar
author_sort	Hamid, Hasnidar
title	Detection Of Triglyceride Based On Microwave Dielectric Properties Of Triglyceride Enzymatic Reaction
title_short	Detection Of Triglyceride Based On Microwave Dielectric Properties Of Triglyceride Enzymatic Reaction
title_full	Detection Of Triglyceride Based On Microwave Dielectric Properties Of Triglyceride Enzymatic Reaction
title_fullStr	Detection Of Triglyceride Based On Microwave Dielectric Properties Of Triglyceride Enzymatic Reaction
title_full_unstemmed	Detection Of Triglyceride Based On Microwave Dielectric Properties Of Triglyceride Enzymatic Reaction
title_sort	detection of triglyceride based on microwave dielectric properties of triglyceride enzymatic reaction
granting_institution	Universiti Putra Malaysia
granting_department	Faculty Of Science
publishDate	2010
url	http://psasir.upm.edu.my/id/eprint/12361/1/FS_2010_18A.pdf
_version_	1747811358056906752

Detection Of Triglyceride Based On Microwave Dielectric Properties Of Triglyceride Enzymatic Reaction

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